99mTc Bağlı Yeni Bir Peptid: Ratlarda 99m Tc β-Kazomorphin 6 Molekülünün Biyodistrübisyon ve Görüntüleme Çalışması

Abstract

Citation of ThisTc β -casomorphin 6, biodistribution and imaging studies on rats. Kafkas Univ Vet Fak Derg, 23, 15-22, 2017. DOI: 10.9775/kvfd.2016 AbstractPeptide radiopharmaceuticals have an increasing significance in nuclear medicine practice. β-casomorphin is a digestive peptide with 6 amino acids (Tyr-Pro-Phe-Pro-Gly-Pro). N terminal amino acid chain mainly tyr-pro-phe-pro structured exogen opioid peptid type beta cosomorphin are μ-receptor agonistic activity with priority. Animal studies show that β-casomorphins can act as opioid receptor agonists. The aim of this study was to label β-casomorphin with 99m Tc and to examine its usefulness as an opioid receptor binding radiopharmaceutical in Albino Wistar rats and cancer cells. β-casomorphin was labeled with 99m Tc radionuclide using bifunctional chelating agent. Quality control studies were done by Instant Thin layer chromatography (ITLC) and High performance liquid chromatography (HPLC) methods. Binding efficiency of the compound was more than 99%. It was observed as stable for at least 3 h at room temperature. Lipophilicity was also performed for labeled molecule. Imaging studies for 99m Tc labeled molecule was done in rats by using gamma camera. For biodistribution studies; 99m Tc labeled molecule was injected to the rats from tail vein and radioactivity per gr weight of each organ was measured as count per second (cps). Receptor specificity was evaluated in imaging and biodistribution studies in experimental animals. Cell labeling studies were also performed on breast and ovarien cancer cells. In terms of evaluating the biodistribution of 99m Tc-β-casomorphin molecule in rats, uterus and ovary displayed high involvement. It was also confirmed by cell labeling studies. If the radiopharmaceutical is radiolabeled with therapeutic radionuclides it would be useful for therapy for uterus, ovary and breast tumors. Keywords Tc β-Kazomorfin 6 Molekülünün Biyodağılım ve Görüntüleme Çalışması ÖzetPeptit radyofarmasötikleri nükleer tıp pratiğinde giderek artan bir öneme sahiptir. β-kazomorfin 6 amino asit (Tyr-Pro-Phe-Pro-GIy-Pro) ile bir sindirim peptitidir. Tyr-Pro-Phe-Pro aminoasit zinciri ile başlayan ekzojen opioid peptid türevi β-kazomorfinler öncelikle μ-resptör agonistik aktiviteye sahiptir. Hayvan çalışmaları β-kazomorfin türevlerinin bir opioid reseptör agonisti olduğunu göstermiştir. Bu çalışmanın amacı, β-kazomorfin'i 99m Tc ile işaretleyerek opioid reseptörler radyofarmasötiği olarak kullanabilirliğini Albino Wistar ratlarda ve kanser hücrelerinde incelemektir. β-kazomorphin bifonksiyonel molekül kullanılarak 99m Tc radyonüklidi ile işaretlendi. Kalite kontrol çalışmaları, ince tabaka kromatografisi (ITLC) ve yüksek performanslı sıvı kromatografisi (HPLC) yöntemleri ile yapıldı. Bileşiğin işaretlenme verimi %99'dan fazla idi. Oda sıcaklığında, en az 3 saat boyunca stabil kaldığı tespit edildi. İşaretli molekülün lipofilite çalışması da gerçekleştirildi. Tc ile işaretli molekülün görüntüleme çalışmaları sıçanlarda gamma kamera kullanılarak gerçekleşti...

99mTc-Trimethoprim’in Radyosentezi ve Biyodağılımı: Deney Hayvanları Kullanılarak Bakteriyel Enfeksiyon Görüntüleme Için Yeni Bir Radyoişaretli Antibiyotik

“…In the stomach the activity initially was quite low but within 120 min it reached 208.3±11.8%. The disappearance of the Tc-TMH complex in the Staphylococcus aureus (SA) infected muscle of the model rat [31][32][33] .…”

“…For this reason, there is a need to develop alternative strategies. Imaging of inflammations with radiopharmaceuticals is the most challenging technique due to its specificity at infection sites [28,33] . When preparing the radiopharmaceuticals, various radionuclides (Ga-67, Tc-99m, In-111, F-18, etc.)…”