97. Biomolecules for the improvement of cryopreservation of human endothelial cells

Sun, Hongyan; Hofmann, Nicola; Glasmacher, Birgit

doi:10.1016/j.cryobiol.2009.10.111

Cited by 18 publications

(26 citation statements)

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“…ectoine or glycine betaine [2]. Cryobiologists have been using CSs like dimethyl sulfoxide (DMSO) and glycerol [3,10] for decades.…”

Section: Introductionmentioning

confidence: 99%

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Compatible solutes: Thermodynamic properties relevant for effective protection against osmotic stress

Held

Sadowski

2016

Fluid Phase Equilibria

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“…ectoine or glycine betaine [2]. Cryobiologists have been using CSs like dimethyl sulfoxide (DMSO) and glycerol [3,10] for decades.…”

Section: Introductionmentioning

confidence: 99%

“…CSs are used in nature against stresses due to heat [2], freezing [3], and denaturants [4], against salt-induced osmotic stress [2,5], against proteolysis [6] and other stresses (e.g. [2,7,8]), and they are also used to promote biological processes (e.g.…”

Section: Introductionmentioning

confidence: 99%

Compatible solutes: Thermodynamic properties relevant for effective protection against osmotic stress

Held

Sadowski

2016

Fluid Phase Equilibria

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“…24 Samples were frozen in a programmable freezer CM2000 (Carburos Metalicos, Spain). The chamber was first cooled to 1°C and held for 10 min, allowing the samples to equilibrate with the chamber temperature.…”

Section: Cryopreservationmentioning

confidence: 99%

“…A previous study from our group showed that supplementation of freezing media with ectoine and L-proline improved cell survival. 24 One aim of the current study was to investigate if thermal activation could protect human pulmonary microvascular endothelial cells from the damaging effects of cryopreservation. Endothelial cells are of great interest in transplantation and cell-based therapy and are often cryopreserved before they are cultured onto scaffolds prior to therapeutic applications.…”

Section: Introductionmentioning

confidence: 99%

Thermal Pretreatment Improves Viability of Cryopreserved Human Endothelial Cells

Hofmann

Sun

Chatterjee

et al. 2015

Biopreservation and Biobanking

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A high survival rate of cryopreserved cells requires optimal cooling and thawing rates in the presence of a cryoprotective agent (CPA) or a combination of CPAs in adequate concentrations. One of the most widely used CPAs, dimethyl sulfoxide (Me2SO), however is toxic at high concentrations and has detrimental effects on cellular functions. Additional processing steps are necessary to remove the CPA after thawing, which make the process expensive and time consuming. Therefore it is of great interest to develop new cryoprotective strategies to replace the currently used CPAs or to reduce their concentration. The aim of this study was to investigate if thermal activation of human pulmonary microvascular endothelial cells (HPMEC ST-1.6R), prior to cryopreservation, could improve their post-thaw viability since the resulting heat shock protein expression acts as an intrinsic cellular protection mechanism. The results of this study suggest that both heat and cold shock pretreatments improve cryopreservation outcome of the HPMEC ST-1.6R cells. By re-cultivating cells after heat shock treatment before cryopreservation, a significant increase in cellular membrane integrity and adherence capacity could be achieved. However a combination of thermal activation and cryopreservation with alternative CPAs such as ectoine and L-proline could not further enhance the cell viability. The results of this study showed that pretreatment of endothelial cells with thermal activation could be used to reduce the Me2SO concentration required in order to preserve cell viability after cryopreservation.

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“…Their 10 % foetal bovine serum medium containing a combination of hydroxyl ethyl starch, ectoine, and co-enzyme Q10 yielded the highest, 93 % viability of mononuclear cells. Sun et al (46) reported that both ectoine and hydroxyectoine protected human endothelial cell line HPMEC-ST1.6R or mesenchymal stem cells and suggested that they may be used in cryobiology as an alternative to the toxic dimethyl sulphoxide (DMSO). One study on rats (47) showed that adding hydroxyectoine to the histidine-tryptophan-ketoglutarate solution reduces the cold ischaemic preservation injury of livers donated after cardiac death and used for transplantation.…”

Section: Cryoprotectionmentioning

confidence: 99%

Ectoine as a promising protective agent in humans and animals

Bownik

Stępniewska

2016

Archives of Industrial Hygiene and Toxicology

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Ectoine is a compatible water molecule-binding solute (osmoprotectant) produced by several bacterial species in response to osmotic stress and unfavourable environmental conditions. This amino acid derivative can accumulate inside cells at high concentrations without interfering with natural processes and can protect the cell against radiation or osmotic stress. This brief review presents the current state of knowledge about the effects of ectoine on animals and focuses on its practical use for enzyme stabilisation, human skin protection, anti-inflammatory treatment, inhibitory effects in neurodegenerative diseases, and other therapeutic potential in human or veterinary medicine.

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97. Biomolecules for the improvement of cryopreservation of human endothelial cells

Cited by 18 publications

References 0 publications

Compatible solutes: Thermodynamic properties relevant for effective protection against osmotic stress

Compatible solutes: Thermodynamic properties relevant for effective protection against osmotic stress

Thermal Pretreatment Improves Viability of Cryopreserved Human Endothelial Cells

Ectoine as a promising protective agent in humans and animals

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