[91] Phosphorylase b and a isozyme I from rabbit heart

Enzymatic Synthesis of Galβ1 → 3 GlcNAc Derivatives Utilising a Phosphorylase fromBifidobacterium bifidum20082

“…The activating group at the anomeric centre of the donor is either a nucleoside diphosphate in the case of the Leloir transferases or a simple sugar-1-phosphate in the case of the non-Leloir glycosyltransferases. [8][9][10] Examples of enzymes from the non-Leloir glycosyl transferase class are phosphorylases, [11][12][13][14][15][16][17] cyclodextrin glycosyltransferases, [18][19][20] and lipid A synthetase. 21 This report describes syntheses employing a non-Leloir enzyme, the b-1AE3-galactosyl-N-acetylhexosamine phosphorylase from Bifidobacterium bifidum DSM 20082 to give the following Galp-b-(1AE3) derivatives: b-D-…”