90-Day Oral Toxicity Assessment of <i>Tropaeolum majus</i> L. in Rodents and Lagomorphs

Araújo, Valdinei de Oliveira; Andreotti, Carlos Eduardo Linhares; Reis, Michelle de Paula; Lima, Daniely Alves de; Pauli, Karoline Bach; Nunes, Bruna; Gomes, Caroline de Barros; Germano, Ricardo de Melo; Cardozo, Euclides Lara; Gasparotto, Arquimedes; Lourenço, Emerson Luiz Botelho

doi:10.1089/jmf.2017.0128

Cited by 3 publications

(2 citation statements)

References 18 publications

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“…Approximately 5 mL of blood was collected (by the decapitation method) into tubules with a clotting agent and a polymer gel for serum separation The biochemical parameters analyzed were: urea, creatinine, total protein, albumin, globulins, aspartate amino transferase (AST), alanine amino transferase (ALT), gamma glutamyl transpeptidase (GGT), alkaline phosphatase (AP), total bilirubin (TB), thyroid stimulating hormone (TSH), free T3, lipid profile (HDL-cholesterol, LDL-cholesterol, VLDL-cholesterol, total cholesterol (TC), and triglycerides (TG)), progesterone, and estradiol. Analyses were performed on an automated biochemical analyzer (Selectra E, Vital Scientific, The Netherlands) [28].…”

Section: Biochemical Analysismentioning

confidence: 99%

“…The samples were properly homogenized and stored under refrigeration (about 4 • C) until processing. The total number of red blood cells (mm 3 ), hemoglobin (g/dL), hematocrit (%), mean corpuscular volume (MCV, fL), mean corpuscular hemoglobin (MCM, pG), mean corpuscular hemoglobin concentration (HCCM, g/dL), platelets (mm 3 ), monocytes (mm 3 ), segmented (mm 3 ), lymphocytes (mm 3 ), and eosinophils (mm 3 ) was measured in an automated hematology analyzer (Abbott Cell Dyn 3500, Abbott Diagnostics, Irving, TX, USA) [28].…”

Section: Hematological Investigationmentioning

confidence: 99%

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Safety Investigations of Two Formulations for Vaginal Use Obtained from Eugenia uniflora L. Leaves in Female Rats

et al. 2022

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Medicinal plants have great prominence in research into the development of new medicines. Eugenia uniflora L. (Myrtaceae) is an edible and medicinal plant with economic value in the northeast region of Brazil. Several preparations from E. uniflora leaves and its fruits are employed as a source of nutrients and bioactive compounds. In this study we evaluated the preclinical toxicology of crude extract and vaginal gel obtained from the leaves of E. uniflora (5%, 10%, and 15%) aiming to provide safety for its use in the treatment of vulvovaginitis. Both formulations were applied to the vaginal cavity for 14 days. Detailed observations of the vaginal region, including pruritus, swelling, irritation, burning, pain, and vaginal secretion, as well as the estrous cycle were evaluated. On the fifth day, blood samples were obtained from the supraorbital plexus for biochemical and hematological analyses. The animals were subsequently euthanized. All animals underwent necropsy and macroscopic examination of the vaginal mucosa and reproductive system. A histological examination was also performed. No clinically significant changes were detected during the entire experimental period. All biochemical, hematological, or histopathological parameters were within the normal range for the species. The data obtained allow us to suggest that the E. uniflora vaginal formulations are safe in this experimental model.

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Section: Biochemical Analysismentioning

confidence: 99%

Section: Hematological Investigationmentioning

confidence: 99%

Safety Investigations of Two Formulations for Vaginal Use Obtained from Eugenia uniflora L. Leaves in Female Rats

et al. 2022

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Sub-chronic oral toxicity study of the alkaloid rich fraction from Luffa cylindrica fruit in Sprague-Dawley rats

Singh S,

Vellapandian

2024

Toxicology Reports

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Preventive effect of total flavonoids of Trollius altaicus on a chronic obstructive pulmonary disease rat model based on the TLR4/NF-κB pathway

Li¹,

Zhao²,

Shao³

et al. 2022

Ann Transl Med

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Background: Chronic obstructive pulmonary disease (COPD) is diagnosed based on the clinical symptoms, risk factors, and pulmonary function tests. Exposure to cigarette smoke (CS), microbial infection stimulates monocytes and macrophages to rapidly synthesise and release inflammatory factors. A previous study of Trollius altaicus (TAF) revealed that it had significant anti-inflammatory and anti-oxidation effects on a pneumonia disease. Based on recent studies of the inflammatory pathway of toll-like receptor 4 (TLR4)/ nuclear factor kappa B (NF-κB), we will explore the influence of TAF on COPD. Methods: Wistar rats were randomly divided into blank control group, Model Group, low-dose TAF + model group, middle-dose TAF + model group, high-dose TAF + model group, positive control + model group. Except for the blank control group, COPD inflammation models were established in all groups by CS poisoning and LPS. Prior to the daily poisoning, 125 mg/kg, 250 mg/kg, and 500 mg/kg of TAF were administered by gavage in the low-, middle-, and high-dose groups, respectively, and dexamethasone solution (1 mg/kg, once daily) was administered continuously in the positive control group on the last 5 days of modelling. General signs, lung function indices, lung imaging results, complete blood count, lung histopathological changes, inflammatory factors in the alveolar lavage fluid, relative expressions of TLR4, IκB kinase α (IKKα), p65, as well as IL-1β proteins and their mRNA relative expressions were measured and compared between each group. Results: Compared with the blank control group, TAF effectively reduced pulmonary parenchymal oedema (wet-to-dry ratio) and respiratory secretions. It also significantly delayed lung function injury. Lung X-ray imaging and haematoxylin and eosin staining. Blood routine examination results showed that TAF effectively inhibited the increase of white blood cell, lymphocyte, and eosinophil counts, decreased the release of inflammatory cytokines [IL-1β, IL-6, IL-8, TNF-α, and transforming growth factor-1β (TGF-1β)] and promoted the release of IL-10. It also inhibited the relative expressions of TLR4, IKKα, p65, IL-1β proteins, as well as IL-1 receptor-associated kinase (IRAK-1), IKKɑ, p65, and IL-1β mRNA. Conclusions: Early intervention of TAF can reduce the occurrence of COPD, reduce the development of inflammation via TLR/NF-κB pathway, and provide reference for further study of the medicinal value of TAF.

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90-Day Oral Toxicity Assessment of Tropaeolum majus L. in Rodents and Lagomorphs

Cited by 3 publications

References 18 publications

Safety Investigations of Two Formulations for Vaginal Use Obtained from Eugenia uniflora L. Leaves in Female Rats

Safety Investigations of Two Formulations for Vaginal Use Obtained from Eugenia uniflora L. Leaves in Female Rats

Sub-chronic oral toxicity study of the alkaloid rich fraction from Luffa cylindrica fruit in Sprague-Dawley rats

Preventive effect of total flavonoids of Trollius altaicus on a chronic obstructive pulmonary disease rat model based on the TLR4/NF-κB pathway

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