872 The comparison of intraperitoneal and intratesticular ozone therapy for testicular ischemiareperfusion injury in rats

Mete, Fatih; Tarhan, Hüseyin; ÇELİK>, Ömer; Ekin, Rahmi Gökhan; Akarken, İlker; Aydemir, Işıl; Vural, Kamıl; İlbey, Yusuf Özlem

doi:10.1016/s1569-9056(15)60860-0

Cited by 4 publications

(6 citation statements)

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“…The tunica albuginea was used to individually weigh testes. As described in a previous study, the left testis samples were examined by biochemical analysis and the right testis samples were examined histopathologically (10). Briefly, the samples were fixed in Bouin's solution and dehydrated in alcohol for 24 hours.…”

Section: Histopathological Examinationmentioning

confidence: 99%

“…Oxidative damage occurs when there is a shift in the balance between production of ROS and the antioxidant defense mechanism in favor of ROS (8,9). Ozone is an inorganic molecular colorless gas composed of three O 2 atoms with a significant pungent odor at room temperature (9,10,11). After ozone therapy (OT) administration, it dissolves in biological water and instantly reacts with antioxidant mechanisms.…”

Section: Introductionmentioning

confidence: 99%

“…During these fast reactions, ozone is neutralized by activation of antioxidant mechanisms, which are superoxide dismutase (SOD), catalase, and glutathione (GSH) peroxidase (GPx) (11). The stimulation of endogenous antioxidants prepares the host defense against ROS (9,10,11). The other important effects of ozone are immune modulation, neoangiogenesis and increased tissue oxygenation (11).…”

Section: Introductionmentioning

confidence: 99%

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Does Ozone Administration Have a Protective Effect Against Cisplatininduced Histological Changes in Rat Testis?

Aydoğdu¹,

Ekin²,

Yıldız³

et al. 2019

jus

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Objective:We investigated the protective and therapeutic effects of ozone therapy (OT) on cisplatin (CP)-induced testicular damage. Materials and Methods: Thirty healthy adult male Wistar rats were divided into five groups consisting of 6 animals each: 1) control, 2) CP, 3) OT, 4) OT + CP and 5) CP + OT groups. Histopathological findings, Johnsen scores, thiobarbituric acid-reactive substances (TBARS), glutathione (GSH), superoxide dismutase (SOD), catalase, and GSH peroxidase (GPx) levels were evaluated. Results: CP caused a significant decrease in testicular weight and Johnsen score compared to the control group. In addition, TBARS level was significantly higher, whereas GSH, SOD, catalase and GPx levels were significantly lower in the CP group when compared to the control group. Pre-and post-CP OT significantly increased GSH, SOD, catalase and GPx levels and decreased TBARS level. Also, testicular weight and Johnsen score were increased with OT. Conclusion: The present study showed that OT is protective against CP-induced testicular damage. OT may be beneficial to patients who underwent CP chemotherapy. Abstract Öz What's known on the subject? and What does the study add?There is currently no study of the protective effect of ozone treatment against cisplatin-induced testicular damages.Amaç: Bu çalışmada, ozon tedavisinin (OT) cisplatin tedavisine (CT) bağlı gelişen testis hasarını önleyici ya da tedavi edici etkisini araştırdık. Gereç ve Yöntem: Wistar cinsi 30 sağlıklı yetişkin erkek rat, her biri 6 rattan oluşan 5 gruba ayrıldı: 1) Kontrol, 2) CT, 3) OT, 4) OT + CT ve 5) CT + OT grubu. Histopatolojik bulgular, Johnsen skorları, tiyobarbitürik asit reaktif maddeler (TBARM), glutatyon (G), süperoksit dismutaz (SOD), katalaz, G peroksidaz (GP) düzeyleri değerlendirildi. Bulgular: CP grubu, kontrol grubuna kıyasla testis ağırlığında ve Johnsen skorunda belirgin bir düşüşe neden oldu. Ayrıca, TBARM düzeyi anlamlı derecede yüksek iken; G, SOD, katalaz ve GP düzeyleri CP grubunda kontrol grubuna göre anlamlı derecede düşük bulundu. CP + OT öncesi ve sonrası GSH, SOD, katalaz ve GP düzeylerini önemli ölçüde artırdı ve TBARM seviyesini düşürdü. Testikül ağırlığı ve Johnsen skoru OT ile arttı.

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Section: Histopathological Examinationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Does Ozone Administration Have a Protective Effect Against Cisplatininduced Histological Changes in Rat Testis?

Aydoğdu¹,

Ekin²,

Yıldız³

et al. 2019

jus

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“…Another study (Mete et al, 2017), compared the efficacy of intra-testicular ozone with intraperitoneal ozone treatment in rats for the prevention of I/R injury. Three-month old male Wistar rats (n = 24) were divided into T/D group, T/D + intraperitoneal ozone group, or T/D + intra-testicular ozone group.…”

Section: Effect Of Ozone In Testicular Injury Caused By Testicular Tomentioning

confidence: 99%

Ozone Therapy: Overview of its Potential Utility in Male Reproduction

Merhi¹,

Bazzi²,

Moseley-LaRue³

et al. 2018

American Journal of Immunology

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Ozone (O3), a highly water-soluble inorganic molecule, is a gas made of three atoms of oxygen (O) with a cyclic structure. Ozone can be produced by medical generators from pure oxygen after passing through a high voltage gradient. Ozone therapy (OT) can be given in medical practice via several routes that include transdermal, intramuscular, rectal, nasal, oral, vaginal, intravenous, intra-arterial, intraperitoneal, intra-pleural, topical, dental, intra-discal and by autohemotherapy. Because ozone, a highly reactive molecule, is a potent oxidant and anti-inflammatory agent, it has strong bactericidal, antiviral, anti-fungal and anti-protozoal actions as well as therapeutic effects on the immune system. With its multifaceted route of administration, OThas been used to treat several pathologies that involve the immune system such as cancers, sepsis, abscesses and chronic wounds, skin problems (such as eczema and psoriasis), HIV infection, asthma, arthritis, urologic problems, osteomyelitis and many others. The purpose of this review article is to evaluate the role of OT in the male reproductive system. We performed a review of all available basic science, experimental animal studies and clinical peer-reviewed articles published in PubMed and Google Scholar until November 2018. The literature so far retrieved shows that most studies pertaining to the effect of OT on male reproduction were performed in animals. Results to date show that OT, via improving the immune system, significantly protects testicular function in the setting of testicular torsion/ischemia, protects against the effect of gonadotoxic agents and treats bacterial infections in the semen. This article calls for a need for at least pilot studies in humans using OT in its safest route of administration, which is probably the transdermal one. This would be significant especially considering that male factor infertility constitutes up to one third of couple infertility and it is very common that poor semen parameters are irreversible with medical or surgical treatment, such as varicocele repair or vasectomy reversal.

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“…However, reactive oxygen species (ROS) overproduction-related ischemia reperfusion (I/R) injury may occur during reperfusion, contributing to infertility. 4 I/R damage occurs due to the stimulation of an intracellular cascade involving the activation of neutrophils, infl ammatory cytokines, and free oxygen radicals. 5 Moreover, during testicular I/R injury, newly arrived oxygen becomes toxic to testicular tissue due to ROS accumulation, testicular oxidative damage, changes in seminiferous tubule structure and function, germ cell apoptosis, and spermatogenesis damage.…”

Section: Introductionmentioning

confidence: 99%

Mirisetinin Testiküler İskemi Reperfüzyon ile İndüklenen Testis ve Akciğer Hasarına Karşı Etkileri

Öztürk¹,

Tanyeli²,

Güzel³

et al. 2021

Sakarya Medical Journal

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Objective Testicular torsion may lead to testicular atrophy and male infertility if untreated within some hours. Since ischemia reperfusion (I/R) injury is a reason for the harmful effects during testicular torsion, antioxidant agents are among the targets of science to deal with reactive oxygen species (ROS). As a flavonoid member, myricetin (3, 3, 4, 5, 5, 7-hexahydroxyflavone, MYR) has antioxidant, anticancer, anti-inflammatory, antiviral, and antidiabetic activities. Materials and MethodsIn this experiment 32 Wistar albino male rats were randomly divided into 4 groups (n=8). Group I was defined as the sham group. In group II, I/R group, testicular torsion detorsion was performed. In group III (MYR 25) and group IV (MYR 50), MYR was administrated intraperitoneally at 25 and 50 mg/kg doses, 30 minutes before detorsion. The testicular and lung tissue samples were examined biochemically.Results In both testis and lung tissues, MYR decreased TOS, MDA, and MPO levels at both high and low doses compared to the I/R group. Besides, SOD values increased in MYR treatment groups compared to the I/R group.Conclusion MYR performed promising effects on testicular and lung tissues following testicular I/R injury according to biochemical parameters.

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872 The comparison of intraperitoneal and intratesticular ozone therapy for testicular ischemiareperfusion injury in rats

Cited by 4 publications

References 0 publications

Does Ozone Administration Have a Protective Effect Against Cisplatininduced Histological Changes in Rat Testis?

Does Ozone Administration Have a Protective Effect Against Cisplatininduced Histological Changes in Rat Testis?

Ozone Therapy: Overview of its Potential Utility in Male Reproduction

Mirisetinin Testiküler İskemi Reperfüzyon ile İndüklenen Testis ve Akciğer Hasarına Karşı Etkileri

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