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Лебедев, К. А.; Ponyakina, I. D.; Козаченко, Н. В.

doi:10.1023/a:1007127912441

Cited by 4 publications

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“…Aliquot inoculation was performed in the following selective nutrient media: (i) meat-peptone agar (MPA) for aerobic and facultatively anaerobic bacteria, (ii) BAIRD-PARKER-Agar (Merck, Germany) for Staphylococcus spp., (iii) KF-Streptococcus agar (Merck, Germany) for Streptococcus spp., (iv) MRSA (MRSA, HiMedia, India) for Lactobacillus spp., (v) Bifi dum agar (HiMedia, India) for Bifi dobacterium spp., (vi) Endo (HiMedia, India) for coliform bacteria, (vii) Sabouraud agar (HiMedia, India) for microscopic fungi, and (viii) Pseudomonas agar (HiMedia, India) for Pseudomonas spp. [22][23][24]. Aft er 24 h of incubation, we recorded preliminary data, and aft er 48 h, -the fi nal ones.…”

Section: Results Th E Formation Of Bacterial Vaginitis In the Balb/c ...mentioning

confidence: 99%

Antimicrobial and Immunomodulatory Action of Probiotic Composition of Bacilli on Bacterial Vaginitis in Mice

et al. 2023

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The purpose of this study was to investigate the antimicrobial and immunomodulatory action of a probiotic composition of Bacillus subtilis and B. megatherium strains (UnicaUro, Sirion (Ukraine)) for experimental bacterial vaginitis. Methods. Experimental studies were conducted on female BALB/c mice; we used Staphylococcus aureus strain B-918 (ATCC 6538) to induce bacterial vaginitis. The strain was vaginally introduced into mice before treatment with probiotic bacteria. In the vagina of mice, aerobic and optionally anaerobic bacteria, including representatives of the genera Staphylococcus, Streptococcus, Lactobacillus, Bifidobacterium, Pseudomonas, coliform bacteria, and microscopic fungi were identified in different periods of observation using generally accepted microbiological methods. Serum antibody titer to S. aureus was determined by the bacterial agglutination reaction. The phagocytic activity and oxygen-dependent bactericidal activity of peritoneal exudate macrophages (PEM) were evaluated using generally accepted immunological methods. Results. The formation of bacterial vaginitis in the BALB/c mice line infected with S. aureus B-918 (ATCC 6538) was evidenced by the appearance of external clinical manifestations of the infectious and inflammatory process against the background of the increased number of aerobic and optionally anaerobic microorganisms, including representatives of the genus Staphylococcus and Streptococcus, microscopic fungi, and decreased number of lactobacilli in different observation periods. The probiotic introduction to mice with bacterial vaginitis led to a dynamic change in the vaginal microbiota: the number of aerobic and optionally anaerobic microorganisms decreased, primarily due to the normalization of the number of representatives of Staphylococcus genus accompanied by a decrease in the antibody titer to staphylococcus in the blood serum. The effective therapeutic action of the probiotic was confirmed by the gradual disappearance of the external clinical signs of the infectious-inflammatory process in the vagina against the background of the functional activity of PEM. Conclusions. The probiotic composition of B. subtilis and B. megatherium (UnicaUro, Sirion, Ukraine) is a promising antimicrobial formulation that may be used in the treatment of bacterial vaginitis; however, further studies are required to confirm its therapeutic, antimicrobial, and immunomodulatory efficacy.

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Section: Results Th E Formation Of Bacterial Vaginitis In the Balb/c ...mentioning

confidence: 99%

Antimicrobial and Immunomodulatory Action of Probiotic Composition of Bacilli on Bacterial Vaginitis in Mice

et al. 2023

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“…We used the provocative MGT for studying the reaction to drugs and prosthetic materials in vivo [22,23]. This test was performed when a patient exhibited a low sensitivity to the given allergen in the neutrophil damage reaction in vitro (i.e., when the parameter measured in this test fell into the gray zone).…”

Section: Methodsmentioning

confidence: 99%

Increase in the Incidence of Allergy to Prosthetic Materials and Local Anesthetics: Immunophysiology and Laboratory Diagnostics of Intolerance

Ponyakina¹,

Sagan²,

Лебедев³

2005

Hum Physiol

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Combined analysis of drug sensitivity in vitro and in vivo is necessary for reliable diagnosis of the intolerance to prosthetic materials and local anesthetics as an intrinsic allergic reaction, especially in the cases of generally weak allergic reactions. Changes in peroxidase release from granulocytes in response to a corresponding substance is an effective in vitro test, and the provocative mucosal-gingival test (MGT) is used in vivo. During the past decade, a growth was observed in the population developing allergies to prosthetic materials and local anesthetics, which is expressed in an increased number of patients with intolerance to these materials against the background of increasing hyperresponsiveness to the drugs which have usually been tolerated well, as well as an increased proportion of patients with a polyvalent allergy to many prosthetic materials and anesthetics. At the same time, the amount of weak responses to drugs in the in vitro test has increased. Some of these patients exhibit a pathologic response to the given drug, which can be detected using the MGT. In most cases, polyvalent allergies to prosthetic materials or anesthetics and a pathological response accompanied by a weak in vitro reaction are found in patients with bronchial asthma and other chronic allergies, chronic candidosis of the oral cavity, and chronic Helicobacter and Giardia lamblia infections.

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“…3. Иммуноглобулины класса А были исследованы в двух формахсекреторной, которая является наиболее эффективным гуморальным фактором так называемого мукозального иммунитета, и мономерной, наличие которой все больше рассматривается как деградация димерной молекулы секреторного IgА (sIgA) [13]. Представленные в таблице данные отражают лишь суммарный уровень изменений защитных белков, обладающих различными свойствами при этих вариантах стимулирующего воздействия.…”

Section: характер изменений факторов местного иммунитета при проведении тестов на функциональное состояние миндалин у больных хтunclassified

Immunodiagnostics, immunotherapy and immunoprophylaxis in otorhinolaryngological practice. Report 3. Diagnostic approaches to the palatine tonsils functional state assessment for selecting a treatment strategy for patients with chronic tonsillitis (analytical summary)

Melnikov

Zabolotny

Bredun

et al. 2021

OTORHINOLARYNGOLOGY

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Experimental work carried out over the past 20 years to study the activity of various functional groups in tonsillar cells in patients with chronic tonsillitis (CT) under local exposure to various immune response modifiers showed that: - tonsillar cells from CT patients in 69.5% of cases are capable of increasing their immunofunctional activity under the influence of immune modifiers in vitro reactions; - the most active of the group of immunotropic immune response modifiers are T-activin and Thymogen; - it can be considered the optimal diagnostic technique to carry out a "stress" test on the tonsil tissue using nonspecific (alternating magnetic field of industrial (low) frequency and low-frequency ultrasound) and specific — a microbial vaccine when applied to the tonsil tissue; - a non-invasive method for recording changes in the tonsils after stimulation may be fluctuations in the level of secretory immunoglobulin A and α-interferon in saliva after the test; - an increase in the level of secretory immunoglobulin A and α-interferon by more than 1/3 of the initial level after the test is a clinically favorable sign and a recommendation for medical treatment to the managing of patients with CT.

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Cited by 4 publications

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Antimicrobial and Immunomodulatory Action of Probiotic Composition of Bacilli on Bacterial Vaginitis in Mice

Antimicrobial and Immunomodulatory Action of Probiotic Composition of Bacilli on Bacterial Vaginitis in Mice

Increase in the Incidence of Allergy to Prosthetic Materials and Local Anesthetics: Immunophysiology and Laboratory Diagnostics of Intolerance

Immunodiagnostics, immunotherapy and immunoprophylaxis in otorhinolaryngological practice. Report 3. Diagnostic approaches to the palatine tonsils functional state assessment for selecting a treatment strategy for patients with chronic tonsillitis (analytical summary)

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