8-Oxo-7,8-Dihydro-2′-Deoxyguanosine (8-oxodG) and 8-Hydroxy-2′-Deoxyguanosine (8-OHdG) as a Potential Biomarker for Gestational Diabetes Mellitus (GDM) Development

Urbaniak, Sandra K; Boguszewska, Karolina; Szewczuk, Michał; Kaźmierczak-Barańska, Julia; Karwowski, Bolesław T.

doi:10.3390/molecules25010202

Cited by 64 publications

(44 citation statements)

References 78 publications

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“…The last one results from the binding of the ● OH to the eighth carbon of the guanine [ 96 ]. 8-OHdG transforms into its oxidised form 8-oxo-7,8-dihydro-2′-deoxyguanosine (8-oxodG) through a keto-enol tautomerism reaction, which is considered the most abundant oxidative injury, with about 100,000 8-oxodG generated every day in a single cell [ 97 , 98 ].…”

Section: Reactive Oxygen Species and Antioxidantsmentioning

confidence: 99%

Telomere Length and Oxidative Stress and Its Relation with Metabolic Syndrome Components in the Aging

Gavia-García

Rosado-Pérez

Arista-Ugalde

et al. 2021

Biology

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A great amount of scientific evidence supports that Oxidative Stress (OxS) can contribute to telomeric attrition and also plays an important role in the development of certain age-related diseases, among them the metabolic syndrome (MetS), which is characterised by clinical and biochemical alterations such as obesity, dyslipidaemia, arterial hypertension, hyperglycaemia, and insulin resistance, all of which are considered as risk factors for type 2 diabetes mellitus (T2DM) and cardiovascular diseases, which are associated in turn with an increase of OxS. In this sense, we review scientific evidence that supports the association between OxS with telomere length (TL) dynamics and the relationship with MetS components in aging. It was analysed whether each MetS component affects the telomere length separately or if they all affect it together. Likewise, this review provides a summary of the structure and function of telomeres and telomerase, the mechanisms of telomeric DNA repair, how telomere length may influence the fate of cells or be linked to inflammation and the development of age-related diseases, and finally, how the lifestyles can affect telomere length.

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Section: Reactive Oxygen Species and Antioxidantsmentioning

confidence: 99%

Telomere Length and Oxidative Stress and Its Relation with Metabolic Syndrome Components in the Aging

Gavia-García

Rosado-Pérez

Arista-Ugalde

et al. 2021

Biology

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“…Promotion of oxidation of protein intensifies during GDM, in which the hyperglycemic state causes protein hydroperoxides, protein carbonyls, C-reactive protein and glycated hemoglobin (HbA1c). In addition to this, it is considered an important mediator of adipocyte disorders, intensifying the inflammatory response and contributing to the complications of diabetes 30 .…”

Section: Discussionmentioning

confidence: 99%

Spectrochemical differentiation in gestational diabetes mellitus based on attenuated total reflection Fourier-transform infrared (ATR-FTIR) spectroscopy and multivariate analysis

Bernardes-Oliveira

Freitas

Morais

et al. 2020

Sci Rep

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Gestational diabetes mellitus (GDM) is a hyperglycaemic imbalance first recognized during pregnancy, and affects up to 22% of pregnancies worldwide, bringing negative maternal–fetal consequences in the short- and long-term. In order to better characterize GDM in pregnant women, 100 blood plasma samples (50 GDM and 50 healthy pregnant control group) were submitted Attenuated Total Reflection Fourier-transform infrared (ATR-FTIR) spectroscopy, using chemometric approaches, including feature selection algorithms associated with discriminant analysis, such as Linear Discriminant Analysis (LDA), Quadratic Discriminant Analysis (QDA) and Support Vector Machines (SVM), analyzed in the biofingerprint region between 1800 and 900 cm−1 followed by Savitzky–Golay smoothing, baseline correction and normalization to Amide-I band (~ 1650 cm−1). An initial exploratory analysis of the data by Principal Component Analysis (PCA) showed a separation tendency between the two groups, which were then classified by supervised algorithms. Overall, the results obtained by Genetic Algorithm Linear Discriminant Analysis (GA-LDA) were the most satisfactory, with an accuracy, sensitivity and specificity of 100%. The spectral features responsible for group differentiation were attributed mainly to the lipid/protein regions (1462–1747 cm−1). These findings demonstrate, for the first time, the potential of ATR-FTIR spectroscopy combined with multivariate analysis as a screening tool for fast and low-cost GDM detection.

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“…Recently it has been speculated that 15-F 2t -IsoP excretion is associated with 7,8-dihydro-8-oxo-2′-deoxyguanosine (8-oxo-dGuo or 8-oxo-dG) [ 19 , 20 ], a guanine modification induced by free radicals and adopted as a non-invasive biomarker of OS [ 21 ]. The accumulation of such damages can lead to genetic information changes, and, consequently, to mutagenesis and cells apoptosis [ 22 ]. The urinary concentration of this biomarker reflects the equilibrium between its production and repair in both DNA and the nucleotide pool [ 23 ].…”

Section: Introductionmentioning

confidence: 99%

Oxidative stress induction in woodworkers occupationally exposed to wood dust and formaldehyde

Ghelli

Bellisario

Squillacioti

et al. 2021

J Occup Med Toxicol

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Background Many workers are exposed to wood dust (WD) and formaldehyde (FA), whose carcinogenic activity is supposed to be oxidative stress-mediated. This study aims to assess to what extent the occupational exposure to WD and FA, albeit within regulatory limits, could result in OS induction in a woodworkers’ population. Methods The sample population consisted of 127 woodworkers from 4 factories and 111 unexposed controls. Individual exposure was assessed by personal air-samplers. Each participant enrolled in the study filled out a questionnaire and provided a urinary sample to quantify OS biomarkers, namely 15-F2t-IsoProstane (15-F2t-IsoP) and 7,8-dihydro-8-oxo-2′-deoxyguanosine (8-oxo-dGuo). The main confounding factor for OS, i.e. tobacco smoking exposure, was assessed by measuring cotinine in urine samples. Results Woodworkers were exposed to significantly higher amounts of WD and FA as compared to controls (p < 0.001). Among OS biomarkers, 15-F2t-IsoP showed statistically significant higher values in woodworkers compared to controls (p = 0.004). A significant, positive correlation was observed between 15-F2t-IsoP and 8-oxo-dGuo (p = 0.005), cotinine (p = 0.05), FA (p < 0.001) and WD (p = 0.01); 8-oxo-dGuo was significantly correlated with cotinine (p = 0.001) and WD (p = 0.004). In addition, WD and FA were significantly correlated each other (p < 0.001). Conclusions The study confirms that WD and FA may induce OS in woodworkers, and highlights that even the compliance with occupational exposure limits can result in measurable biological outcomes.

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8-Oxo-7,8-Dihydro-2′-Deoxyguanosine (8-oxodG) and 8-Hydroxy-2′-Deoxyguanosine (8-OHdG) as a Potential Biomarker for Gestational Diabetes Mellitus (GDM) Development

Cited by 64 publications

References 78 publications

Telomere Length and Oxidative Stress and Its Relation with Metabolic Syndrome Components in the Aging

Telomere Length and Oxidative Stress and Its Relation with Metabolic Syndrome Components in the Aging

Spectrochemical differentiation in gestational diabetes mellitus based on attenuated total reflection Fourier-transform infrared (ATR-FTIR) spectroscopy and multivariate analysis

Oxidative stress induction in woodworkers occupationally exposed to wood dust and formaldehyde

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