[8] Membrane immunoassay: A spin membrane immunoassay for thyroxine

Tan, C. T.; Chan, S.W.; Hsia, J.C.

doi:10.1016/0076-6879(81)74010-2

Cited by 22 publications

(2 citation statements)

References 9 publications

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“…Because of their ability to carry different agents in the aqueous cavity, many substances such as fluorophores, oligonucleotides, enzymes or drugs have been encapsulated in the liposomes during their formation and utilized in diagnostics, gene delivery, drug delivery, adjuvant formulation, cosmetic skin condition [2][3][4][5][6][7]. In detection assay systems, liposomes have been widely used as carriers of markers to enhance detection single.…”

Section: Introductionmentioning

confidence: 99%

An electrochemical immunosensor based on enzyme-encapsulated liposomes and biocatalytic metal deposition

Guo

Chu

et al. 2010

Analytica Chimica Acta

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Section: Introductionmentioning

confidence: 99%

An electrochemical immunosensor based on enzyme-encapsulated liposomes and biocatalytic metal deposition

Guo

Chu

et al. 2010

Analytica Chimica Acta

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“…Since Haxby et al [1] initially reported that liposomes are capable of being used for trace biomolecule investigation, several liposome-based immunoassay designs have been reported [2][3][4][5][6]. Generally, the methods reported rely on the ability of liposomes to sequester signal-generating molecules and subsequently release them in proportion to the concentration of analyte being measured.…”

mentioning

confidence: 99%

Liposome-based flow injection enzyme immunoassay for theophylline

Durst

1990

Mikrochim Acta

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Preliminary results are presented on the development of a sensitive, quantitative immunoassay based on a regenerable, flow injection analysis system incorporating a double-amplification approach. The double amplification is achieved by means of liposome-encapsulated peroxidase enzyme molecules which are released subsequent to a competitive immunological reaction with analyte molecules for immobilized antibodies. The released peroxidase enzymatically cleaves, from an organofluorine substrate, fluoride ions which are then potentiometrically measured. The entire process is carried out in a flow injection analysis system. The competition between the analyte molecules (theophylline) and theophylline-derivatized liposomes for immobilized antibody sites in flowthrough immunoreactor column results in unbound liposomes being carried downstream where they are ruptured in the presence of hydrogen peroxide and p-fluorophenol. The peroxidase molecules released react enzymatically to produce fluoride ions which are measured with an ion-selective electrode. The immunoreactor column is then regenerated with a chaotropic agent and the next sample or calibration solution is injected. By means of column regeneration and calibration, accurate quantitation can be achieved; a feature missing from conventional batch-type immunoassays. By means of this liposome/enzyme doubleamplification approach, theophylline was determined over a range of concentrations from 0.2 to 4000 ng/ml. The detection limit of 200 pg/ml corresponds to about 100 femtomole of theophylline measured in the 100 #1 sample injected.A double-amplification immunoassay for theophylline, based on the use of peroxidase-loaded liposomes and a packed-bed antibody reactor column in a flow

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Automated nephelometric immunoassays with novel shell/core particles

Kapmeyer¹,

Pauly²,

Tuengler³

1988

Clinical Laboratory Analysis

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Nephelometry has become a very reliable and convenient method for immunoassays. Recent innovations in polymer chemistry have led to the design of highly sensitive and reproducible immunoassays for the Behring Nephelometer Analyzer. The principle of particle‐enhanced immunoassays has led to the development of new polymer particles. Key advances include the preparation and use of particles with polystyrene cores covered by thin shells with chemically reactive groups. The cores are prepared by emulsion polymerization, giving particles with a well‐defined size. The particle size has been optimized to fulfill the requirements for nephelometric analysis. The shells consist of a polystyrene/polymethacrylate/polymethacrylamidoacet‐aldehyde dipentylacetal copolymer, which allows a covalent binding of the immunochemicals of interest. Using these particles, a method for the determination of C‐reactive protein has been worked out.

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[8] Membrane immunoassay: A spin membrane immunoassay for thyroxine

Cited by 22 publications

References 9 publications

An electrochemical immunosensor based on enzyme-encapsulated liposomes and biocatalytic metal deposition

An electrochemical immunosensor based on enzyme-encapsulated liposomes and biocatalytic metal deposition

Liposome-based flow injection enzyme immunoassay for theophylline

Automated nephelometric immunoassays with novel shell/core particles

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