An electrochemical immunosensor based on enzyme-encapsulated liposomes and biocatalytic metal deposition

Ultrasensitive electrochemical immunosensor for PSA biomarker detection in prostate cancer cells using gold nanoparticles/PAMAM dendrimer loaded with enzyme linked aptamer as integrated triple signal amplification strategy

“…4A shows the DPV response of the immunosensor in the presence of 10 ml of T-PER containing extracted PSA from various numbers of cancer cells. As illustrated the peak current increased with the increasing numbers of cancer (Ning et al, 2010) DVP 0.2 0.5-40 GS e /Qntum dot S W V f [47] 0.003 0.005-10 GCE/ILs g /CNTs DPV h [30] 0.020 0.2-40 Microarray-based platform (Lee et al, 2015) Fluorescence -0.01-100 Graphene-modified GC (Xu et al, 2011) ECL i 0.008 0.010-8 AuNP j / FITC k (Choi et al, 2013) Fluorescence -0.01-100 GCE/GS/Th l Amperometry 0.001 0.002-10 GCE/Gr m /Gold Composite (Jang et al, 2015)[52] DVP 0.59 up to 10 Silicon nitride layer (Koukouvinos et al, 2011) R e flectance spectroscopy 0.15 0.5-100 GDE n /cysteamine (Qu et al, 2010) L (Fig. 4B).…”

Electrochemical Biosensing in Cancer Diagnostics and Follow‐up

“…Basically, the modified sensing surface is treated with glutaraldehyde (or other aldehydes) that is allowed to react with the antibodies’ functional groups. Like this, the binding between the surface and the biological compound is accomplished through the crosslinking agent , .…”

“…Qu et al. used cysteamine to produce a SAM on the sensor platform and covalently bound the capture antibody through glutaraldehyde. Emami et al.…”

“… for PSA detection was performed by Qu et al. . However, in the latter study, AP was encapsulated in liposomes.…”

Multiplexed electrochemical immunoassay using streptavidin/nanogold/carbon nanohorn as a signal tag to induce silver deposition

“…Various immunosensor arrays combining with electrochemical [3][4][5][6] and optical [7][8][9][10] readout have been designed for simultaneous detection of multiplex analytes. Owing to the unique advantages of easy preparation, low cost, high sensitivity and good portability [11][12][13], electrochemical sensor array, especially those fabricated on the disposable screen-printed electrode strips, have gained considerable attention for their potential application in point-of-care treatment [5].…”