8,8″-Biapigeninyl stimulates osteoblast functions and inhibits osteoclast and adipocyte functions: Osteoprotective action of 8,8″-biapigeninyl in ovariectomized mice

Biomedicinal and Chemical Profile of Cupressus sempervirens: A Mini Review

“…Chemical studies on CS revealed the presence of polyphenols (1-7), flavonoids (8) and flavone glycosides (9)(10)(11)(12). Bioflavonoid including amentoflavone, cupressuflavone, 4″′-mono-Omethyl amentoflavone, hinokiflavone, isocryptomerin and podocarpusflavone A has also been reported from this plant (13)(14)(15)(16)(17) [4]. As per reported in several studies, essential oils from cones and fruits mainly contained volatile monoterpenes (18-28) and sesquiterpenes (29-32) [5-8].…”

“…Moreover, Khan et al also investigated the antiosteoporotic activity of sugiol, trans-communic acid, 15-acetoxy imbricatolic acid and imbricatolic acid isolated from this plant. Among them, sugeol at a dose of 1.0 mg/kg body weight exhibited significant osteoprotective effects and did not show uterine estrogenicity at the same dose [17,18].…”

A naturally occurring rare analog of quercetin promotes peak bone mass achievement and exerts anabolic effect on osteoporotic bone

“…Mineralization of RCOs was performed following previously published protocol [2,40]. Briefly, RCO were seeded onto 12-well plates (25,000 cells/well) in osteoblast differentiation medium.…”

“…The fixed cells were stained with alizarin red-S (40 mM, pH 4.5) for 30 min followed by washing with H 2 O [41]. Stained cells were first photographed under light microscope, and alizarin stain was then extracted by 10% (v/v) acetic acid with shaking at room temperature for 30 min [40]. Cells were scrapped out of the culture plates, centrifuged (20,000×g for 15 min), and supernatant was collected.…”

“…To the supernatant, 10% (v/v) ammonium hydroxide was added to bring the pH of the solution to 4.5. Absorbance of the solution was read at 405 nm [2,40,42].…”