Abstract:The hydroxylation of dehydroepiandrosterone (DHEA) to 7α -hydroxy-5-androstene-17-one (7α-OH-DHEA) and 7β-hydroxy-5-androstene-17-one (7β-OH-DHEA) by Gibberella sp. CICC 2498 and Absidia coerulea CICC 41050 was investigated. The media ingredients were optimized. Single factors such as the DHEA concentration, culture time, medium volume, and inoculum rate were individually investigated to generate optimum biotransformation conditions. An orthogonal optimization process using a four-factor, three- level L9 (33) … Show more
Biocatalysis, which can be performed by whole cells and isolated enzymes, has become a topic of public interest for its potential use in the chemical industry in manufacturing, monitoring, and waste management [...]
Biocatalysis, which can be performed by whole cells and isolated enzymes, has become a topic of public interest for its potential use in the chemical industry in manufacturing, monitoring, and waste management [...]
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