Microbial Biocatalysis

Pan, Tao; Wang, Zhilong

doi:10.3390/catal13030629

Cited by 2 publications

(1 citation statement)

References 15 publications

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“…Whole-cell biocatalysts can be used for different types of processes, such as biotransformation, biodegradation, and fermentation. These processes can involve one or more steps to produce the desired chemicals [ 3 , 4 ].…”

Section: Introductionmentioning

confidence: 99%

Antimicrobial Activity and Molecular Docking Studies of the Biotransformation of Diterpene Acanthoic Acid Using the Fungus Xylaria sp.

Marinho,

de Oliveira,

Silva-Silva

et al. 2023

Antibiotics

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Biotransformations are reactions mediated by microorganisms, such as fungi. These bioreactions have high chemo- and stereoselectivity on organic substrates and can be applied in the search for new bioactive compounds. In this study, acanthoic acid (AA) was biotransformed using the fungus Xylaria sp., giving the novel compound 3β,7β-dihydroxyacanthoic acid (S1). Both the AA and the product S1 were tested against Gram-positive and Gram-negative bacteria. To identify and validate possible biological targets as enzymes or proteins involved in the activity observed in vitro, we used the molecular docking method. Hydroxylation at the C-3 and C-7 positions of the biotransformation product enhanced its activity against Escherichia coli as well as its binding affinity and interactions with superoxide dismutase 1 (SOD1; PDB ID 4A7G). Based on our results, the SOD1 enzyme was suggested to be a possible target for the antioxidant activity of product S1.

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Section: Introductionmentioning

confidence: 99%

Antimicrobial Activity and Molecular Docking Studies of the Biotransformation of Diterpene Acanthoic Acid Using the Fungus Xylaria sp.

Marinho,

de Oliveira,

Silva-Silva

et al. 2023

Antibiotics

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Biodiesel Production from Waste Cooking Oil Using Recombinant Escherichia coli Cells Immobilized into Fe3O4–Chitosan Magnetic Microspheres

Zhao,

Han,

Zhou

et al. 2024

Molecules

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Developing reusable and easy-to-operate biocatalysts is of significant interest in biodiesel production. Here, magnetic whole-cell catalysts constructed through immobilizing recombinant Escherichia coli cells (containing MAS1 lipase) into Fe3O4–chitosan magnetic microspheres (termed MWCC@MAS1) were used for fatty acid methyl ester (FAME) production from waste cooking oil (WCO). During the preparation process of immobilized cells, the effects of chitosan concentration and cell concentration on their activity and activity recovery were investigated. Optimal immobilization was achieved with 3% (w/v) chitosan solution and 10 mg wet cell/mL cell suspension. Magnetic immobilization endowed the whole-cell catalysts with superparamagnetism and improved their methanol tolerance, enhancing the recyclability of the biocatalysts. Additionally, we studied the effects of catalyst loading, water content, methanol content, and reaction temperature on FAME yield, optimizing these parameters using response surface methodology and Box–Behnken design. An experimental FAME yield of 89.19% was gained under the optimized conditions (3.9 wt% catalyst loading, 22.3% (v/w) water content, 23.0% (v/w) methanol content, and 32 °C) for 48 h. MWCC@MAS1 demonstrated superior recyclability compared to its whole-cell form, maintaining about 86% of its initial productivity after 10 cycles, whereas the whole-cell form lost nearly half after just five cycles. These results suggest that MWCC@MAS1 has great potential for the industrial production of biodiesel.

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Microbial Biocatalysis

Abstract: Biocatalysis, which can be performed by whole cells and isolated enzymes, has become a topic of public interest for its potential use in the chemical industry in manufacturing, monitoring, and waste management [...]

Cited by 2 publications

References 15 publications

Antimicrobial Activity and Molecular Docking Studies of the Biotransformation of Diterpene Acanthoic Acid Using the Fungus Xylaria sp.

Antimicrobial Activity and Molecular Docking Studies of the Biotransformation of Diterpene Acanthoic Acid Using the Fungus Xylaria sp.

Biodiesel Production from Waste Cooking Oil Using Recombinant Escherichia coli Cells Immobilized into Fe3O4–Chitosan Magnetic Microspheres

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