67‐kDa Laminin receptor contributes to hypoxia‐induced migration and invasion of trophoblast‐like cells by mediating matrix metalloproteinase‐9

Wang, Leilei; Yu, Yang; Guan, Hongyan; Liu, Tong; Qiao, Chong

doi:10.1111/1440-1681.12389

Cited by 13 publications

(11 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In addition, infection with human cytomegalovirus has been confirmed to inhibit extravillous cytotrophoblast migration and invasion through the dysregulation of CXCR4/CXCL12 signaling throughout early pregnancy ( 32 ). Consistent with several previous studies ( 6 , 33 , 34 ), the present study found that the migration and invasion of trophoblast cells were enhanced following exposure to 3% O 2 . Therefore, it was hypothesized that the expression of CXCR4 in trophoblasts depends on physiological hypoxia in early pregnancy.…”

Section: Discussionsupporting

confidence: 93%

Hypoxia‑induced expression of CXCR4 favors trophoblast cell migration and invasion via the activation of HIF‑1α

Zhang

Wang

et al. 2018

Int J Mol Med

View full text Add to dashboard Cite

The placenta initially develops in a low-oxygen environment up to week 8–10 of gestation, and a low oxygen level is a critical factor in the regulation of trophoblast migration and invasion. CXC chemokine receptor 4 (CXCR4) is transcriptionally activated by hypoxia in cancer cells. However, whether CXCR4 is involved in hypoxia-inducible factor (HIF)-1α-dependent trophoblastic migration and invasion in a physiologically hypoxic environment (3% O2) remains to be fully elucidated and requires further investigation. In the present study, the expression of CXCR4 in first-trimester villi was investigated, as was the response of the trophoblast to hypoxia, and the role of CXCR4 and HIF-1α in trophoblast migration and invasion. CXCR4 was significantly elevated in the first-trimester villi compared with normal full-term placentas. In vitro, the expression of CXCR4 at the mRNA and protein levels was increased in JEG3 cells exposed to 3% O2 in a time-dependent manner, and the migratory and invasive abilities of the JEG3 cells were upregulated. In addition, CXCR4 knockdown by transfection with CXCR4-specific small interfering (si)RNA decreased the migration and invasion of JEG3 cells exposed to 3% O2. Furthermore, synthetic siRNA specific for HIF-1α significantly suppressed the expression of CXCR4 in JEG3 cells exposed to 3% O2, whereas pcDNA-HIF-1α significantly increased the expression of CXCR4. These results indicated that the hypoxia-induced expression of CXCR4 promoted trophoblast cell migration and invasion via the activation of HIF-1α, which is crucial during placentation.

show abstract

Section: Discussionsupporting

confidence: 93%

Hypoxia‑induced expression of CXCR4 favors trophoblast cell migration and invasion via the activation of HIF‑1α

Zhang

Wang

et al. 2018

Int J Mol Med

View full text Add to dashboard Cite

show abstract

“…26 Published studies have established the significance of BM proteins in placentation and embryogenesis in murine BM-proteins ablated models, 23 human placental tissue, 25,48,49 and cytotrophoblast invasion in vitro. 25,50,51 However, none of the studies to date have directly determined the impact of The MMP2 expressions for HMW-pECM was lower than that of complete pECM with no significant differences. LMW-pECM did not have a significant impact on the MMP2 expression (n 5 3).…”

Section: Discussionmentioning

confidence: 97%

Placental basement membrane proteins are required for effective cytotrophoblast invasion in a three‐dimensional bioprinted placenta model

Kuo

Guo

Cabrera-Luque

et al. 2018

J Biomedical Materials Res

View full text Add to dashboard Cite

Fetal cytotrophoblast invasion of maternal decidual vasculature is necessary to normal pregnancy. In preeclampsia, there is shallow invasion and abnormal remodeling of the uterine vasculature that lead to significant maternal and perinatal morbidity and mortality. The placental basement membrane (BM) proteins (e.g., laminin and collagen) has been implicated in the development of placenta while the level of laminin is significantly lower in preeclampsia. However, there are very limited studies, if any, on the effect of extracellular matrix (ECM) microenvironment on the invasion of cytotrophoblast. In this study, we hypothesized that placental BM proteins are required for effective cytotrophoblast invasion. Using proteomics, we found that more than 80% of ECM proteins in placental basal plate (pECM) were BM proteins. In addition to upregulating expressions of MMP2 (1.5-fold) and MMP9 (6.3-fold), pECM significantly increased the motility rates of cytotrophoblasts by 13-fold (from 5.60 ± 0.95 to 75.5 ± 21.8 µm/day) to achieve an effective invasion rate that was comparable to in vivo results. Treatments with PI3K inhibitors completely removed the pECM-enhanced invasive phenotypes and genotypes of cytotrophoblasts, suggesting its dominant role in cytotrophoblast-ECM interactions. Our results described, for the first time, the substantial effects of the ECM microenvironment on regulating cytotrophoblast invasion, an area that is less investigated but appear to be critical in the pathogenesis of preeclampsia. Moreover, the approach presented in this work that fabricates organ models with organ-specific ECM can be an attractive option to screen and develop novel therapeutics and biomarkers not only in preeclampsia but also other diseases such as cancer metastasis. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 106A: 1476-1487, 2018.

show abstract

“…Remarkably, some important extracellular factors, such as TGF-β, FGF and Wnt, participate in the regulatory network by inducing EMT and/or the expression of MMPs [ 23 – 27 , 29 , 40 , 41 ]. The mechanisms of hypoxia in this regulatory network are similar to those of extracellular factors, as shown in Figure 1 [ 28 , 29 , 42 , 43 ].…”

Section: The Mechanisms Underlying Emt and Their Role In Metastasismentioning

confidence: 99%

Epithelial-to-mesenchymal transition, circulating tumor cells and cancer metastasis: Mechanisms and clinical applications

2017

View full text Add to dashboard Cite

show abstract

67‐kDa Laminin receptor contributes to hypoxia‐induced migration and invasion of trophoblast‐like cells by mediating matrix metalloproteinase‐9

Cited by 13 publications

References 36 publications

Hypoxia‑induced expression of CXCR4 favors trophoblast cell migration and invasion via the activation of HIF‑1α

Hypoxia‑induced expression of CXCR4 favors trophoblast cell migration and invasion via the activation of HIF‑1α

Placental basement membrane proteins are required for effective cytotrophoblast invasion in a three‐dimensional bioprinted placenta model

Epithelial-to-mesenchymal transition, circulating tumor cells and cancer metastasis: Mechanisms and clinical applications

Contact Info

Product

Resources

About