Power unevenness, mainly induced by stimulated Raman scattering, has been a major problem in multi-band transmission systems, especially in the upcoming field-deployed 400 Gbit/s widened C band plus L band system for backbone long-haul and ultra-long-haul scenarios. To reduce the impact of power unevenness, we propose an automatic power optimization (APO) algorithm to guarantee reliable transmission for all channels, especially the channels at short wavelengths. The simulation results show that the power unevenness of output power after 5-span transmission in the C band is up to 11 dB before APO, while after APO is applied, it is greatly improved to less than 1.6 dB. To further investigate the performance of the APO scheme, we conduct a real-time 44 Tbit/s C+L transmission system over 1900 km G.654.E fiber utilizing 400 Gbit/s transponders. The experimental results show that the power unevenness has been effectively compensated from 12 dB to 4 dB in the entire 11 THz range, making the received optical signal-to-noise ratio relatively flat (3.4 dB). Moreover, the capacity and distance product of this system is 83.6 Pbit/s·km (44 Tbit/s × 1900 km), and to the best of our knowledge, this is a record capacity and distance product in the real-time single-mode fiber transmission system.