6-Bromoindole Derivatives from the Icelandic Marine Sponge Geodia barretti: Isolation and Anti-Inflammatory Activity

Di, Xiaxia; Rouger, Caroline; Harðardóttir, Ingibjörg; Freysdóttir, Jona; Molinski, Tadeusz F.; Taşdemir, Deniz; Ómarsdóttir, Sesselja

doi:10.3390/md16110437

Cited by 32 publications

(29 citation statements)

References 56 publications

(77 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In this study, we selected three species of Geodia because there was little intraspecific variation when cultured in M1 medium. In addition, G. barretti is the source of compounds with anti-inflammatory activity 29 . Cell density of G. barretti , Geodia sp., and G. neptuni was measured with a finer time resolution to understand the dynamics of cell division for each species (Fig.…”

Section: Resultsmentioning

confidence: 99%

Breakthrough in Marine Invertebrate Cell Culture: Sponge Cells Divide Rapidly in Improved Nutrient Medium

Conkling

Hesp

Munroe

et al. 2019

Sci Rep

View full text Add to dashboard Cite

Sponges (phylum porifera) are among the oldest Metazoa and considered critical to understanding animal evolution and development. They are also the most prolific source of marine-derived chemicals with pharmaceutical relevance. Cell lines are important tools for research in many disciplines, and have been established for many organisms, including freshwater and terrestrial invertebrates. Despite many efforts over multiple decades, there are still no cell lines for marine invertebrates. In this study, we report a breakthrough: we demonstrate that an amino acid-optimized nutrient medium stimulates rapid cell division in 9 sponge species. The fastest dividing cells doubled in less than 1 hour. Cultures of 3 species were subcultured from 3 to 5 times, with an average of 5.99 population doublings after subculturing, and a lifespan from 21 to 35 days. Our results form the basis for developing marine invertebrate cell models to better understand early animal evolution, determine the role of secondary metabolites, and predict the impact of climate change to coral reef community ecology. Furthermore, sponge cell lines can be used to scale-up production of sponge-derived chemicals for clinical trials and develop new drugs to combat cancer and other diseases. Sponges (Phylum Porifera) are key components of many benthic marine ecosystems. There are more than 9,000 described species that occur worldwide, from the intertidal to the deep sea 1. Among the oldest metazoans, sponges have evolved a variety of strategies to adapt to different environments. Because they are sessile as adults, they have evolved sophisticated chemical systems for communication, defense from predators, antifoulants to prevent other organisms from growing over them, and to prevent infection from microbes filtered out of the water 2,3. These chemicals interact with molecules that have been conserved throughout evolutionary history and are involved in human disease processes, for example, cell cycling 4 , immune and inflammatory responses 5 , and calcium and sodium regulation 6,7. Vertebrate, insect, and plant cell lines are important tools for research in many disciplines, including human health, evolutionary and developmental biology, agriculture, and toxicology. Although cell lines have been established for freshwater and terrestrial invertebrates (e.g., Hydra, Caenorhabditis), and long-term (>1 month) primary cultures have been reported for cells derived from tissues of the cnidarian Anemonia viridis and the shrimp Penaeus 8,9 , attempts to establish cell lines from marine invertebrates have been unsuccessful 9-11. Marine sponges, including some of the species in this study, are the source of thousands of novel chemicals with pharmaceutically relevant properties 12-14. Supply of these chemicals is a bottleneck to development of sponge-derived drug leads: wild harvest is not ecologically sustainable, and chemical synthesis is challenging due to the complexity of many of the bioactive chemical compounds. In vitro production has been proposed as an option...

show abstract

Section: Resultsmentioning

confidence: 99%

Breakthrough in Marine Invertebrate Cell Culture: Sponge Cells Divide Rapidly in Improved Nutrient Medium

Conkling

Hesp

Munroe

et al. 2019

Sci Rep

View full text Add to dashboard Cite

show abstract

“…The activity of these alkaloids as P2X7 receptor antagonists may therefore have broad therapeutic possibilities for inflammatory and immune-mediated diseases. The 6-bromoindole derivatives geobarettin B (18) and C (19), the 6-bromoindole alkaloids 6-bromoconicamin (20), and barettin (13), obtained from the sponge G. barretti, showed an anti-inflammatory effect on LPS-, TNF-α-, and IL-1β-stimulated dendritic cells, reducing IL-12p40 secretion [82]. Geobarettin C was also able to increase the levels of IL-10 secreted by these cells.…”

Section: Sponge-derived Alkaloidsmentioning

confidence: 99%

Marine Alkaloids with Anti-Inflammatory Activity: Current Knowledge and Future Perspectives

2020

View full text Add to dashboard Cite

Alkaloids are nitrogenous compounds with various biological activities. Alkaloids with anti-inflammatory activity are commonly found in terrestrial plants, but there are few records of the identification and characterization of the activity of these compounds in marine organisms such as fungi, bacteria, sponges, ascidians, and cnidarians. Seaweed are a source of several already elucidated bioactive compounds, but few studies have described and characterized the activity of seaweed alkaloids with anti-inflammatory properties. In this review, we have gathered the current knowledge about marine alkaloids with anti-inflammatory activity and suggest future perspectives for the study and bioprospecting of these compounds.

show abstract

“…Natural compounds with antimicrobial, anti-inflammatory and anticancer activities have been isolated from marine invertebrates in the Arctic region [ 3 , 51 , 52 , 53 ] and in the marine Antarctic environment [ 54 , 55 , 56 , 57 ].…”

Section: Marine Compounds From Polar Regionsmentioning

confidence: 99%

Marine Terpenoids from Polar Latitudes and Their Potential Applications in Biotechnology

et al. 2020

View full text Add to dashboard Cite

Polar marine biota have adapted to thrive under one of the ocean’s most inhospitable scenarios, where extremes of temperature, light photoperiod and ice disturbance, along with ecological interactions, have selected species with a unique suite of secondary metabolites. Organisms of Arctic and Antarctic oceans are prolific sources of natural products, exhibiting wide structural diversity and remarkable bioactivities for human applications. Chemical skeletons belonging to terpene families are the most commonly found compounds, whereas cytotoxic antimicrobial properties, the capacity to prevent infections, are the most widely reported activities from these environments. This review firstly summarizes the regulations on access and benefit sharing requirements for research in polar environments. Then it provides an overview of the natural product arsenal from Antarctic and Arctic marine organisms that displays promising uses for fighting human disease. Microbes, such as bacteria and fungi, and macroorganisms, such as sponges, macroalgae, ascidians, corals, bryozoans, echinoderms and mollusks, are the main focus of this review. The biological origin, the structure of terpenes and terpenoids, derivatives and their biotechnological potential are described. This survey aims to highlight the chemical diversity of marine polar life and the versatility of this group of biomolecules, in an effort to encourage further research in drug discovery.

show abstract

6-Bromoindole Derivatives from the Icelandic Marine Sponge Geodia barretti: Isolation and Anti-Inflammatory Activity

Cited by 32 publications

References 56 publications

Breakthrough in Marine Invertebrate Cell Culture: Sponge Cells Divide Rapidly in Improved Nutrient Medium

Breakthrough in Marine Invertebrate Cell Culture: Sponge Cells Divide Rapidly in Improved Nutrient Medium

Marine Alkaloids with Anti-Inflammatory Activity: Current Knowledge and Future Perspectives

Marine Terpenoids from Polar Latitudes and Their Potential Applications in Biotechnology

Contact Info

Product

Resources

About