56. Evaluation of a new solution for hypothermic machine perfusion (HMP) of the liver. II – A study in the perfused rat liver in vitro

Carnevale, Matías Emanuel; Balaban, Cecilia Lucía; Guibert, Edgardo E.; Bottai, Hebe; Rodriguez, Joaquı́n V.

doi:10.1016/j.cryobiol.2012.07.057

Cited by 3 publications

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“…These solutions, designed to counteract the effects of hypothermia, are made up of different components, but all contain three essential constituents: adequate electrolytes (high or low Na + /K + ratio), an impermeant compound to prevent cell swelling, and a buffer to prevent acidosis (10). In our laboratory, we have developed an HMP solution based on N,N-bis-2-hydroxyethyl-2-aminoethanesulfonic acid (BES), gluconate, and polyethylene glycol (BGP-HMP); this solution was previously assayed in an in vitro model (11,12), and its efficiency was compared versus the classic solution developed by Bretschneider (13).…”

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Hypothermic Machine Perfusion Versus Cold Storage in the Rescuing of Livers From Non‐Heart‐Beating Donor Rats

et al. 2013

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The aim of this work was to compare the efficiency of cold storage (CS) and hypothermic machine perfusion (HMP) methods of preserving grafts excised from non-heart-beating donors that had suffered 45 minutes of warm ischemia. We developed a new solution for HMP to use in liver transplantation, based on BES, gluconate, and polyethylene glycol (BGP-HMP solution). After 24 h of HMP or CS, livers were reperfused at 37°C with Krebs-Henseleit solution with added dextran. For both procedures, portal pressure and flow were measured and the intrahepatic resistance (IR) was calculated. The pH oscillations and enzyme activities (LDH, AST, and ALT) were evaluated for the perfusion buffer during normothermic reperfusion. O2 consumption of the liver, glycogen production, and bile flow were also measured during the normothermic reperfusion period. Portal flow and IR showed statistical differences (P < 0.05) between the two groups (n = 5). HMP with BGP-HMP solution resulted in higher values of portal flow and lower IR than CS with HTK solution. Enzyme release after 90 min of reperfusion did not show statistical differences between groups. With regard to bile flow and O2 consumption, livers preserved by both processes were able to produce bile, but livers preserved with HMP were able to take up more O2 than livers preserved by CS.

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Hypothermic Machine Perfusion Versus Cold Storage in the Rescuing of Livers From Non‐Heart‐Beating Donor Rats

et al. 2013

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“…The BGP-HMP solution was developed for hypothermic perfusion [145] and also tested for static preservation [147], the oncotic agent used was PEG 35000 at a concentration of 1 g/L. Figure 4 shows the effect of temperature on BG-basic solution viscosity at different PEG concentrations.…”

Section: Experimental Observations About the Viscosity Of The Perfusimentioning

confidence: 99%

“…Their physicochemical properties have been studied and compared with other traditional OPS [79]. Experimental COP values obtained for the newly formulated gluconate-based BG-PEG and BG-HES preservation solutions [145] were determined at different concentrations of two different colloids: PEG 35000 and HES used at 1, 2, 5, 10, and 20 g/L at 20°C. The results are presented in Figure 2.…”

Section: Experimental Observations Of Temperature On Liver Preservatimentioning

confidence: 99%

“…Our group has been interested in developing a new group of OPS formulated on gluconate and the Good’s buffer BES (N,N-bis[2-hydroxyethyl]-2-aminoethanesulfonic acid) with different colloids (e.g., BES gluconate HES-BG-HES [145]). Their physicochemical properties have been studied and compared with other traditional OPS [79].…”

Section: Experimental Observations Of Temperature On Liver Preservatimentioning

confidence: 99%

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Organ Preservation into the 2020s: The Era of Dynamic Intervention

Petrenko

Carnevale

Somov

et al. 2019

Transfus Med Hemother

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Organ preservation has been of major importance ever since transplantation developed into a global clinical activity. The relatively simple procedures were developed on a basic comprehension of low-temperature biology as related to organs outside the body. In the past decade, there has been a significant increase in knowledge of the sequelae of effects in preserved organs, and how dynamic intervention by perfusion can be used to mitigate injury and improve the quality of the donated organs. The present review focuses on (1) new information about the cell and molecular events impacting on ischemia/reperfusion injury during organ preservation, (2) strategies which use varied compositions and additives in organ preservation solutions to deal with these, (3) clear definitions of the developing protocols for dynamic organ perfusion preservation, (4) information on how the choice of perfusion solutions can impact on desired attributes of dynamic organ perfusion, and (5) summary and future horizons.

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“…We developed an HMP solution based on N , N ‐bis‐2‐hydroxyethyl‐2‐aminoethanesulfonic acid–gluconate–polyethylene glycol (BGP). This solution was previously studied storing rats' livers by HMP, assayed in the in vitro model of isolated perfused rat livers (IPRLs), and its efficiency was compared with the HTK solution . IPRL offers different advantages in resembling in vivo conditions and standardized experimental setups, but nevertheless, it fails to fully translate all of the clinically relevant parameters.…”

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The Novel N,N‐bis‐2‐Hydroxyethyl‐2‐Aminoethanesulfonic Acid–Gluconate–Polyethylene Glycol–Hypothermic Machine Perfusion Solution Improves Static Cold Storage and Reduces Ischemia/Reperfusion Injury in Rat Liver Transplant

et al. 2019

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Organ transplantation is the treatment of choice against terminal and irreversible organ failure. Optimal preservation of the graft is crucial to counteract cold ischemia effects. As we developed an N,N‐bis‐2‐hydroxyethyl‐2‐aminoethanesulfonic acid–gluconate–polyethylene glycol (BGP)–based solution (hypothermic machine perfusion [HMP]), we aimed to analyze the use of this solution on static cold storage (SCS) of rat livers for transplantation as compared with the histidine tryptophan ketoglutarate (HTK) preservation solution. Livers procured from adult male Sprague Dawley rats were preserved with BGP‐HMP or HTK solutions. Liver total water content and metabolites were measured during the SCS at 0°C for 24 hours. The function and viability of the preserved rat livers were first assessed ex vivo after rewarming (90 minutes at 37°C) and in vivo using the experimental model of reduced‐size heterotopic liver transplantation. After SCS, the water and glycogen content in both groups remained unchanged as well as the tissue glutathione concentration. In the ex vivo studies, livers preserved with the BGP‐HMP solution were hemodynamically more efficient and the O2 consumption rate was higher than in livers from the HTK group. Bile production and glycogen content after 90 minutes of normothermic reperfusion was diminished in both groups compared with the control group. Cellular integrity of the BGP‐HMP group was better, and the histological damage was reversible. In the in vivo model, HTK‐preserved livers showed a greater degree of histological injury and higher apoptosis compared with the BGP‐HMP group. In conclusion, our results suggest a better role of the BGP‐HMP solution compared with HTK in preventing ischemia/reperfusion injury in the rat liver model.

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56. Evaluation of a new solution for hypothermic machine perfusion (HMP) of the liver. II – A study in the perfused rat liver in vitro

Cited by 3 publications

References 0 publications

Hypothermic Machine Perfusion Versus Cold Storage in the Rescuing of Livers From Non‐Heart‐Beating Donor Rats

Hypothermic Machine Perfusion Versus Cold Storage in the Rescuing of Livers From Non‐Heart‐Beating Donor Rats

Organ Preservation into the 2020s: The Era of Dynamic Intervention

The Novel N,N‐bis‐2‐Hydroxyethyl‐2‐Aminoethanesulfonic Acid–Gluconate–Polyethylene Glycol–Hypothermic Machine Perfusion Solution Improves Static Cold Storage and Reduces Ischemia/Reperfusion Injury in Rat Liver Transplant

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