55. Evaluation of a new solution for hypothermic machine perfusion (HMP) of the liver. I – Composition and physicochemical parameters

Pascucci, Franco Andrés; Carnevale, Matías Emanuel; Balaban, Cecilia Lucía; Mamprin, María Eugenia; Guibert, Edgardo E.; Rodriguez, Joaquı́n V.

doi:10.1016/j.cryobiol.2012.07.056

Cited by 4 publications

(7 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The composition of Bretschneider solution (Bretschneider's histidine-tryptophan-ketoglutarate [HTK], Franz Kohler Chemie GmbH, Bensheim, Germany) has already been described (13).The BGP-HMP solution (12) was prepared in our laboratory; it had the following composition: 100 mM sodium gluconate, 7 mM potassium gluconate, 20 mM sucrose, 30 mM BES, 2.5 mM KH2PO4, and 0.03 mM polyethylene glycol (35 kDa), 5 mM MgSO4, 3 mM glutathione, 5 mM adenosine, and 15 mM glycine, together with 0.25 mg/mL streptomycin and 10 U/mL penicillin G. Osmolarity was 297 ± 4 mOsm/kg H2O; pH was 7.40; Na + and K + concentrations were 120 ± 2 and 10 ± 1 mEq/L, respectively. Medicinal synthetic air was bubbled through the HTK solution at the selected temperatures at a gas flow of 600 mL/min for 20 min before use.…”

Section: Solutionsmentioning

confidence: 99%

“…These solutions, designed to counteract the effects of hypothermia, are made up of different components, but all contain three essential constituents: adequate electrolytes (high or low Na + /K + ratio), an impermeant compound to prevent cell swelling, and a buffer to prevent acidosis (10). In our laboratory, we have developed an HMP solution based on N,N-bis-2-hydroxyethyl-2-aminoethanesulfonic acid (BES), gluconate, and polyethylene glycol (BGP-HMP); this solution was previously assayed in an in vitro model (11,12), and its efficiency was compared versus the classic solution developed by Bretschneider (13).…”

mentioning

confidence: 99%

See 1 more Smart Citation

Hypothermic Machine Perfusion Versus Cold Storage in the Rescuing of Livers From Non‐Heart‐Beating Donor Rats

et al. 2013

Self Cite

View full text Add to dashboard Cite

The aim of this work was to compare the efficiency of cold storage (CS) and hypothermic machine perfusion (HMP) methods of preserving grafts excised from non-heart-beating donors that had suffered 45 minutes of warm ischemia. We developed a new solution for HMP to use in liver transplantation, based on BES, gluconate, and polyethylene glycol (BGP-HMP solution). After 24 h of HMP or CS, livers were reperfused at 37°C with Krebs-Henseleit solution with added dextran. For both procedures, portal pressure and flow were measured and the intrahepatic resistance (IR) was calculated. The pH oscillations and enzyme activities (LDH, AST, and ALT) were evaluated for the perfusion buffer during normothermic reperfusion. O2 consumption of the liver, glycogen production, and bile flow were also measured during the normothermic reperfusion period. Portal flow and IR showed statistical differences (P < 0.05) between the two groups (n = 5). HMP with BGP-HMP solution resulted in higher values of portal flow and lower IR than CS with HTK solution. Enzyme release after 90 min of reperfusion did not show statistical differences between groups. With regard to bile flow and O2 consumption, livers preserved by both processes were able to produce bile, but livers preserved with HMP were able to take up more O2 than livers preserved by CS.

show abstract

Section: Solutionsmentioning

confidence: 99%

mentioning

confidence: 99%

Hypothermic Machine Perfusion Versus Cold Storage in the Rescuing of Livers From Non‐Heart‐Beating Donor Rats

et al. 2013

Self Cite

View full text Add to dashboard Cite

show abstract

“…The O 2 consumption of isolated rat livers perfused at 5 and 10°C with BGP-HMP or HTK solutions saturated with air was also established [151]. It is necessary to provide appropriate oxygenation of the preservation solution during HMP perfusion protocols as a requirement for effective preservation and maintenance of organ viability and function.…”

Section: Experimental Observations Of Temperature On Liver Preservatimentioning

confidence: 99%

Organ Preservation into the 2020s: The Era of Dynamic Intervention

Petrenko

Carnevale

Somov

et al. 2019

Transfus Med Hemother

View full text Add to dashboard Cite

Organ preservation has been of major importance ever since transplantation developed into a global clinical activity. The relatively simple procedures were developed on a basic comprehension of low-temperature biology as related to organs outside the body. In the past decade, there has been a significant increase in knowledge of the sequelae of effects in preserved organs, and how dynamic intervention by perfusion can be used to mitigate injury and improve the quality of the donated organs. The present review focuses on (1) new information about the cell and molecular events impacting on ischemia/reperfusion injury during organ preservation, (2) strategies which use varied compositions and additives in organ preservation solutions to deal with these, (3) clear definitions of the developing protocols for dynamic organ perfusion preservation, (4) information on how the choice of perfusion solutions can impact on desired attributes of dynamic organ perfusion, and (5) summary and future horizons.

show abstract

“…Solutions designed to counteract the effects of hypothermia are made up of different components, but they all contain 4 essential constituents: Adequate extracellular or intracellular electrolyte concentration (high or low Na + /K + ratio). A buffer to prevent acidosis An impermeant compound to prevent cell swelling. A colloid to minimize the interstitial edema due to the diffusion of the preservation solution into the interstitium …”

mentioning

confidence: 99%

“…A colloid to minimize the interstitial edema due to the diffusion of the preservation solution into the interstitium. (10) The gold standard solution used worldwide was the University of Wisconsin (UW; Viaspan, USA) solution developed in the late 1980s. (5) Since then, other solutions have been introduced in the market, such as the histidine tryptophan ketoglutarate (HTK) solution, developed by Bretschneider, (11) and Celsior, which combines compounds from UW and HTK and was initially used mainly for intrathoracic organs.…”

mentioning

confidence: 99%

The Novel N,N‐bis‐2‐Hydroxyethyl‐2‐Aminoethanesulfonic Acid–Gluconate–Polyethylene Glycol–Hypothermic Machine Perfusion Solution Improves Static Cold Storage and Reduces Ischemia/Reperfusion Injury in Rat Liver Transplant

et al. 2019

Self Cite

View full text Add to dashboard Cite

Organ transplantation is the treatment of choice against terminal and irreversible organ failure. Optimal preservation of the graft is crucial to counteract cold ischemia effects. As we developed an N,N‐bis‐2‐hydroxyethyl‐2‐aminoethanesulfonic acid–gluconate–polyethylene glycol (BGP)–based solution (hypothermic machine perfusion [HMP]), we aimed to analyze the use of this solution on static cold storage (SCS) of rat livers for transplantation as compared with the histidine tryptophan ketoglutarate (HTK) preservation solution. Livers procured from adult male Sprague Dawley rats were preserved with BGP‐HMP or HTK solutions. Liver total water content and metabolites were measured during the SCS at 0°C for 24 hours. The function and viability of the preserved rat livers were first assessed ex vivo after rewarming (90 minutes at 37°C) and in vivo using the experimental model of reduced‐size heterotopic liver transplantation. After SCS, the water and glycogen content in both groups remained unchanged as well as the tissue glutathione concentration. In the ex vivo studies, livers preserved with the BGP‐HMP solution were hemodynamically more efficient and the O2 consumption rate was higher than in livers from the HTK group. Bile production and glycogen content after 90 minutes of normothermic reperfusion was diminished in both groups compared with the control group. Cellular integrity of the BGP‐HMP group was better, and the histological damage was reversible. In the in vivo model, HTK‐preserved livers showed a greater degree of histological injury and higher apoptosis compared with the BGP‐HMP group. In conclusion, our results suggest a better role of the BGP‐HMP solution compared with HTK in preventing ischemia/reperfusion injury in the rat liver model.

show abstract

55. Evaluation of a new solution for hypothermic machine perfusion (HMP) of the liver. I – Composition and physicochemical parameters

Cited by 4 publications

References 0 publications

Hypothermic Machine Perfusion Versus Cold Storage in the Rescuing of Livers From Non‐Heart‐Beating Donor Rats

Hypothermic Machine Perfusion Versus Cold Storage in the Rescuing of Livers From Non‐Heart‐Beating Donor Rats

Organ Preservation into the 2020s: The Era of Dynamic Intervention

The Novel N,N‐bis‐2‐Hydroxyethyl‐2‐Aminoethanesulfonic Acid–Gluconate–Polyethylene Glycol–Hypothermic Machine Perfusion Solution Improves Static Cold Storage and Reduces Ischemia/Reperfusion Injury in Rat Liver Transplant

Contact Info

Product

Resources

About