2000
DOI: 10.1023/a:1007850014021
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Cited by 105 publications
(17 citation statements)
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“…We conducted radioimmunoassays of total androgens (T and DHT) on the unoxidized samples and DHT only assays on the oxidized samples. The crossreactivity between the T-specific antibody and DHT was 46% (see also Nash et al 2000), so we estimated the T content of each sample as totalK0.46DHT). The intra-assay coefficient of variation was 4.3G0.3% (meanGs.e.)…”
Section: Methodsmentioning
confidence: 99%
“…We conducted radioimmunoassays of total androgens (T and DHT) on the unoxidized samples and DHT only assays on the oxidized samples. The crossreactivity between the T-specific antibody and DHT was 46% (see also Nash et al 2000), so we estimated the T content of each sample as totalK0.46DHT). The intra-assay coefficient of variation was 4.3G0.3% (meanGs.e.)…”
Section: Methodsmentioning
confidence: 99%
“…For the quantification of T, E 2 , 11-KT and 17,20β-P in the plasma, already established and well-described enzyme-linked immunoassays (ELISA) were used [74][75][76] with some modifications, using reagents from Cayman Chemical Company (Ann Arbor, MI, USA). For steroid extraction, 200 µL of plasma were extracted twice with 2 mL diethyl ether.…”
Section: Hormone Measurementsmentioning
confidence: 99%
“…Immunoassays (ELISAs) have been recently reported for the determination of estrogens [29][30][31][32][33][34]. Although this method can be sensitive and suitable for the screening of some steroid substances, it may give erroneous results due to poor specificity, accuracy, or reproducibility, as evidenced by the overestimation of trace amounts of estrogens [35][36][37].…”
Section: Introductionmentioning
confidence: 99%