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Lee, Joong B.; Baxi, Mohit K.; Idamakanti, Neeraja; Reddy, P. Seshidhar; Zakhartchouk, Alexandre N.; Pyne, Caron; Babiuk, Lorne A.; Tikoo, Suresh K.

doi:10.1023/a:1008017404513

Cited by 12 publications

(3 citation statements)

References 8 publications

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“…It lies near the right end of the genome (nt 30932c to 33950c) and is transcribed from the l strand of the genome. Nucleotide sequence analysis identified five ORFs, two of which (ORF3 and ORF5) show homologies to the 34K protein (ORF6) of HAVs (38). Transcriptional analysis of the E4 region identified the transcription start and termination sites and splice donor and acceptor sites (38) ( Table 2).…”

Section: Resultsmentioning

confidence: 99%

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Nucleotide Sequence, Genome Organization, and Transcription Map of Bovine Adenovirus Type 3

Reddy

Idamakanti

Zakhartchouk

et al. 1998

J Virol

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The complete DNA sequence of bovine adenovirus type 3 is reported here. The size of the genome is 34,446 bp in length with a G+C content of 54%. All the genes of the early and late regions are present in the expected locations of the genome. However, the late-region genes are organized into seven families, instead of five as they are in human adenovirus type 2. The deduced amino acid sequences of open reading frames (ORFs) in the late regions and early region 2 (E2) and for IVa2 show higher degrees of homology, whereas the predicted amino acid sequences of ORFs in the E1, E3, and E4 regions and the pIX, fiber, and 33,000-molecular-weight nonstructural proteins show little or no homology with the corresponding proteins of other adenoviruses. In addition, the penton base protein lacks the integrin binding motif, RGD, but has an LDV motif instead of an MDV motif. Interestingly, as in other animal adenoviruses, the virus-associated RNA genes appear to be absent from their usual location. Sequence analysis of cDNA clones representing the early- and late-region genes identified splice acceptor and splice donor sites, polyadenylation signals and polyadenylation sites, and tripartite leader sequences.

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Section: Resultsmentioning

confidence: 99%

“…a Detailed analyses of the transcription map of these areas have been described elsewhere(31,38,50). b c, complementary strand.…”

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confidence: 99%

Nucleotide Sequence, Genome Organization, and Transcription Map of Bovine Adenovirus Type 3

Reddy

Idamakanti

Zakhartchouk

et al. 1998

J Virol

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“…Like other adenoviruses, BAV-3 is a nonenveloped icosahedral particle of 75-80 nm in diameter and has a double-stranded linear genomic DNA [10]. The E1, E3, and E4 regions and its complete genome sequence of BAV-3 have been described [6,11,12]. Serologic surveys indicated widespread distribution of BAV throughout the world.…”

Section: Introductionmentioning

confidence: 99%

Isolation, identification, and complete genome sequence of a bovine adenovirus type 3 from cattle in China

Zhu

Zuo

Cai

et al. 2011

Virol J

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BackgroundBovine adenovirus type 3 (BAV-3) belongs to the Mastadenovirus genus of the family Adenoviridae and is involved in respiratory and enteric infections of calves. The isolation of BAV-3 has not been reported prior to this study in China. In 2009, there were many cases in cattle showing similar clinical signs to BAV-3 infection and a virus strain, showing cytopathic effect in Madin-Darby bovine kidney cells, was isolated from a bovine nasal swab collected from feedlot cattle in Heilongjiang Province, China. The isolate was confirmed as a bovine adenovirus type 3 by PCR and immunofluorescence assay, and named as HLJ0955. So far only the complete genome sequence of prototype of BAV-3 WBR-1 strain has been reported. In order to further characterize the Chinese isolate HLJ0955, the complete genome sequence of HLJ0955 was determined.ResultsThe size of the genome of the Chinese isolate HLJ0955 is 34,132 nucleotides in length with a G+C content of 53.6%. The coding sequences for gene regions of HLJ0955 isolate were similar to the prototype of BAV-3 WBR-1 strain, with 80.0-98.6% nucleotide and 87.5-98.8% amino acid identities. The genome of HLJ0955 strain contains 16 regions and four deletions in inverted terminal repeats, E1B region and E4 region, respectively. The complete genome and DNA binding protein gene based phylogenetic analysis with other adenoviruses were performed and the results showed that HLJ0955 isolate belonged to BAV-3 and clustered within the Mastadenovirus genus of the family Adenoviridae.ConclusionsThis is the first study to report the isolation and molecular characterization of BAV-3 from cattle in China. The phylogenetic analysis performed in this study supported the use of the DNA binding protein gene of adenovirus as an appropriate subgenomic target for the classification of different genuses of the family Adenoviridae on the molecular basis. Meanwhile, a large-scale pathogen and serological epidemiological investigations for BVA-3 infection might be carried out in cattle in China. This report will be a good beginning for further studies on BAV-3 in China.

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Construction and Characterization of E3-Deleted Bovine Adenovirus Type 3 Expressing Full-Length and Truncated Form of Bovine Herpesvirus Type 1 Glycoprotein gD

et al. 1998

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Using the homologous recombination machinery of E. coli, a 1.245-kb deletion was introduced in the E3 region of bovine adenovirus 3 (BAV3) genomic DNA cloned in a plasmid. Transfection of the restriction enzyme-excised, linear E3-deleted BAV3 genomic DNA into primary fetal bovine retina cells produced infectious virus (BAV3. E3d), suggesting that all the E3-specific open reading frames are nonessential for virus replication in vitro. Using a similar approach, we constructed replication-competent (BAV3.E3gD and BAV3. E3gDt) BAV3 recombinant expressing full-length (gD) or truncated (gDt) glycoprotein of bovine herpes virus 1. Recombinant gD and gDt proteins expressed by BAV3.E3gD and BAV3.E3gDt, respectively, were recognized by gD-specific monoclonal antibodies directed against conformational epitopes, suggesting that antigenicity of recombinant gD and gDt was similar to that of the native gD expressed in bovine herpes virus 1-infected cells. Intranasal immunization of cotton rats induced strong gD- and BAV3-specific IgA and IgG immune responses. These results suggest that replication-competent bovine adenovirus 3-based vectors have potential for the delivery of vaccine antigens to the mucosal surfaces of animals.

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Cited by 12 publications

References 8 publications

Nucleotide Sequence, Genome Organization, and Transcription Map of Bovine Adenovirus Type 3

Nucleotide Sequence, Genome Organization, and Transcription Map of Bovine Adenovirus Type 3

Isolation, identification, and complete genome sequence of a bovine adenovirus type 3 from cattle in China

Construction and Characterization of E3-Deleted Bovine Adenovirus Type 3 Expressing Full-Length and Truncated Form of Bovine Herpesvirus Type 1 Glycoprotein gD

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