5-Aminosalicylic Acid Loaded Chitosan-Carrageenan Hydrogel Beads with Potential Application for the Treatment of Inflammatory Bowel Disease

Abstract

The aim of our work is to prepare mucoadhesive particles with biopolymers and 5-Aminosalicylic acid (5ASA) using the ionotropic gelation technique to ensure a controlled drug release at the colon level with potential applications in the treatment of intestinal bowel disease (IBD). The preparation of particles through the crosslinking of Chitosan (CS) with sodium tripolyphosphate (TPP) using different mass ratios and the influence of the k-Carrageenan (kCG) layer were studied. UV–VIS spectrometry was employed to assess encapsulation efficiency and drug release profile of 5ASA. The particles were investigated using FT-IR spectrometry for chemical characterization and the DLS results highlighted a monodisperse particle size distribution. The morphology of the polymeric beads was investigated using micro-computer tomography (µCT) and Scanning Electron Microscopy (SEM). Particles based on Chitosan and k-Carrageenan were able to incorporate and preserve 5ASA in an acidic and alkaline medium. The 5ASA loaded polymeric particles obtained after immersion for 1 h in kCG solution exhibited the lowest release rate in pH = 1.2. Biocompatibility studies performed on all of the particles displayed a good viability for the CCD 841 CoN cells and low cytotoxicity. All of the results have shown that these new biomaterials could be a versatile platform of targeted carriers with potential applications in inflammatory bowel disease treatment.

Chitosan/Pluronic F127 Thermosensitive Hydrogel as an Injectable Dexamethasone Delivery Carrier

“…Some strong signals can be observed corresponding to the stretching vibrations of different bonds: 2880 cm −1 (C–H stretch), 1278 and 1240 cm −1 (C–O–C stretches), and 1097 cm −1 (C–O stretch) [ 46 , 47 , 48 ]. On the other hand, in the TPP spectrum ( Figure 2 II(c)), it is possible to observe absorption bands at 1208 cm −1 belonging to the stretching vibrations of P═O, at 1137 cm −1 symmetric and antisymmetric stretching vibrations of the PO 2 group, at 1094 cm −1 symmetric and antisymmetric stretching vibrations of the PO 3 group, and at 889 cm −1 antisymmetric stretching vibrations of the P–O–P bond [ 49 , 50 ]. When d and e spectra belonging to the hydrogels prepared are compared with the a–c spectra of the initial components, it is clearly noted that although the characteristic signals of both initial polymers are observed, their largest amount corresponds to PF, which has the highest proportion in the final material.…”

Natural Gums in Drug-Loaded Micro- and Nanogels

“…Stavarache et al [ 176 ] described mucoadhesive chitosan particles with κ-carrageenan coating attached by polyelectrolyte complexation as potential carrier for 5-aminosalicylic acid. The aim of the study was to deliver the active ingredient to colon without releasing it in upper parts of the gastrointestinal tract.…”

Nanoparticles for oral delivery: targeted therapy for inflammatory bowel disease

“…86 Chitosan NPs are widely applied as colon-targeted DDSs in IBD treatment because of their good biocompatibility, desirable biodegradability, and strong muco-adhesiveness. 69,87,88 Moreover, chitosan NPs presenting a positively charged amino group (–NH 3+ ) can specifically interact with the negatively charged mucosal surface of the inflamed colon contributing to prolonged drug release, and enhanced colonic drug absorption. 89,90 However, chitosan is known for its solubility in acidic media, which may exhibit a premature release and/or burst release of the agents before reaching the colon.…”