5-Aminolevulinic Acid Photodynamic Therapy combined with CO2 laser therapy in treatment of laryngeal papilloma: Case report

Zhang, Yunjie; Yang, Yuguang; Zou, Xianbiao; Huang, Zheng

doi:10.1016/j.pdpdt.2016.03.008

Cited by 15 publications

(14 citation statements)

References 5 publications

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“…Currently, PDT has achieved satisfactory efficacy in treating pediatric laryngeal papilloma, condyloma acuminatum, and acne. 9 - 12 On the basis of the principle of PDT, photosensitization can be selectively enriched in virally infected foci after being applied locally on skin lesions. Under 635nm laser irradiation, it generates singlet oxygen and other active oxides, which can damage cell membranes, mitochondria, and DNA, cause cell necrosis and apoptosis, and then kill diseased cells.…”

Section: Discussionmentioning

confidence: 99%

“…Under 635nm laser irradiation, it generates singlet oxygen and other active oxides, which can damage cell membranes, mitochondria, and DNA, cause cell necrosis and apoptosis, and then kill diseased cells. 12 , 13 In recent years, some researchers have found that PDT shows efficacy by activating local specific immunity. 14 , 15 Brotosik et al found that PDT resulted in apoptosis of epidermal keratinocytes and Langerhans cells.…”

Section: Discussionmentioning

confidence: 99%

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A case series pilot study on the combination of 5-aminolevulinic acid and photodynamic therapy (ALA-PDT) for treatment of vitiligo

Zhang

Lin

Guo

et al. 2018

An. Bras. Dermatol.

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BackgroundTo study the effective therapeutic concentration, drug application duration, irradiation duration and irradiation dosage of 5-aminolevulinic acid photodynamic therapy (ALA-PDT) for treating vitiligo and observe its clinical efficacy.ObjectiveTo assess the clinical efficacy of ALA-PDT for treating vitiligo.MethodsALA with different drug concentrations, drug application durations, irradiation durations and irradiation dosages were adopted to treat skin lesions caused by vitiligo to determine the effective drug concentration, drug application duration, irradiation duration and irradiation dosage for treating three vitiligo volunteers and observe the therapeutic results.ResultsThe clinical trial suggested that ALA at a drug concentration of 1.5%, drug application duration for 3 hours, irradiation dosage of 80mw/cm2 and irradiation duration for 20 min was effective in treating vitiligo. Under these parameters, ALA-PDT was effective to the three vitiligo volunteers, with mild pain and feeling of burning but no other adverse reaction during treatment.Study limitationsDue to the small sample size in this study for the effectiveness of PDT in treating vitiligo and the potential variations in the efficacy for treating the disease at different areas, further studies shall be conducted for confirmation.ConclusionsALA with a drug concentration at 1.5%, drug application duration for 3 hours, irradiation dosage of 80 mw/cm2 and irradiation duration for 20 min is effective in treating vitiligo. Therefore, ALA-PDT is safe and effective in treating the disease, with minor adverse events, providing a new method for treating vitiligo in the future.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

A case series pilot study on the combination of 5-aminolevulinic acid and photodynamic therapy (ALA-PDT) for treatment of vitiligo

Zhang

Lin

Guo

et al. 2018

An. Bras. Dermatol.

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“…However, PDL alone has therapeutic effects on keratosis; therefore, the ability of laryngeal spraying of 5‐ALA to induce sufficient therapeutic effect remains unclear. Zhang et al and Zhou et al reported using topical PDT in the treatment of laryngeal papillomatosis . They used cotton balls or gauze soaked in 5‐ALA solution and directly applied the solution to the surface of lesions for 2 to 3 hours.…”

Section: Introductionmentioning

confidence: 99%

“…Zhang et al and Zhou et al reported using topical PDT in the treatment of laryngeal papillomatosis. 13,17 They used cotton balls or gauze soaked in 5-ALA solution and directly applied the solution to the surface of lesions for 2 to 3 hours. Although a good clinical response was achieved, this procedure significantly extended the duration of surgery.…”

Section: Introductionmentioning

confidence: 99%

Topical Application of 5‐Aminolevulinic Acid Is Sufficient for Photodynamic Therapy on Vocal Folds

et al. 2018

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Objectives To evaluate the feasibility of topical photodynamic therapy (PDT) using 5‐aminolevulinic acid (5‐ALA) for vocal fold leukoplakia. Study Design Ex vivo and in vivo. Methods 5‐ALA was applied topically as a 20% solution to ex vivo canine vocal folds. The penetration depth and concentrations of 5‐ALA in tissue were quantified using frozen sectioning and fluorescamine derivatization after 5‐ALA contact incubation or topical spraying. Then, 5‐ALA solution was sprayed on leporine vocal folds once, twice, or given systemically in vivo. Protoporphyrin IX (PPIX) location was visualized using fluorescence microscopy, and PPIX concentrations were measured using a fluorescent quantitative method. Hematoxylin and eosin (H&E) staining was performed to visualize the histological changes of vocal folds after PDT for each group. Results Topical incubation of 15 minutes with 5‐ALA achieved a penetration depth of over 2 mm and similar concentrations within the superficial 500 μm of epithelium, compared with longer incubation times. Topical spraying of 5‐ALA produced sufficient concentrations in vocal folds, but the retention time is short. An in vivo leporine model showed that laryngeal spraying of 20% 5‐ALA induced similar penetration depth and concentrations of PPIX compared to systemic administration of 5‐ALA. Two sprays of 20% 5‐ALA solution with an interval of 30 minutes are needed to produce complete exfoliation of vocal fold epithelium. Conclusion Topical PDT with laryngeal spraying of 20% 5‐ALA solution achieves sufficient therapeutic effects and is potentially applicable for the treatment of vocal fold leukoplakia. Level of Evidence NA Laryngoscope, 129:E80–E86, 2019

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“…ALA-PDT has been used to cure squamous carcinoma for several decades, and widely applied for treatment of skin diseases, including flat wart [10], actinic keratosis [11] and acne vulgaris [12]. In addition, ALA-PDT can induce or promote various cancer cells apoptosis, including breast cancer [13], pituitary adenomas [14] and laryngeal papilloma [15]. However, there are few reports describing whether ALA-PDT affects scar formation.…”

Section: Introductionmentioning

confidence: 99%

5-Aminolaevulinic Acid-Based Photodynamic Therapy Restrains Pathological Hyperplasia of Fibroblasts

et al. 2017

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BackgroundThis study aimed to explore whether 5-aminolaevulinic acid-based photodynamic therapy (ALA-PDT) restrains pathological hyperplasia of fibroblasts from hyperplastic scar tissues, and to investigate the potential mechanism.Material/MethodsWe used MTT assay, flow cytometry, and terminal-deoxynucleotidyl transferase mediated nick-end labeling (TUNEL) to examine the effects of ALA-PDT on proliferation, cell cycle, and apoptosis of fibroblasts isolated from hyperplastic scar tissues. The growth-promoting effect of fibroblasts on vascular endothelial cells was measured by cell co-culture. Real-time PCR and Western blot analysis were performed to detect the expression levels of transforming growth factor-β1 (TGF-β1), α-smooth muscle actin (α-SMA), Collagen I, Collagen III, vascular endothelial growth factor-A (VEGFA), and basic fibroblast growth factor (bFGF).ResultsALA-PDT inhibited proliferation delayed cell cycle progress, promoted apoptosis of fibroblasts, and suppressed its growth-promoting effect on vascular endothelial cells, and decreased expression of TGF-β1, α-SMA, Collagen I, Collagen III, VEGFA, and bFGF.ConclusionsALA-PDT effectively restrained pathological hyperplasia of fibroblasts from hyperplastic scar tissues, which may provide a research basis for clinical therapy of hyperplastic scars.

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5-Aminolevulinic Acid Photodynamic Therapy combined with CO2 laser therapy in treatment of laryngeal papilloma: Case report

Cited by 15 publications

References 5 publications

A case series pilot study on the combination of 5-aminolevulinic acid and photodynamic therapy (ALA-PDT) for treatment of vitiligo

A case series pilot study on the combination of 5-aminolevulinic acid and photodynamic therapy (ALA-PDT) for treatment of vitiligo

Topical Application of 5‐Aminolevulinic Acid Is Sufficient for Photodynamic Therapy on Vocal Folds

5-Aminolaevulinic Acid-Based Photodynamic Therapy Restrains Pathological Hyperplasia of Fibroblasts

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