5-Amino-2-mercapto-1,3,4-thidiazole modified single-use sensors for electrochemical DNA analysis

Muti, Mihrican; Erdem, Arzum; Karagözler, A. Ersin; Soysal, Mert

doi:10.1016/j.colsurfb.2011.12.021

Cited by 9 publications

(7 citation statements)

References 22 publications

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“…The highest and more repeatable R ct values were obtained by using AuNP-AMT-PGE. It can be explained that amine groups of AMT strongly bind with the carboxyl groups of the activated graphite and the result is the densely populated AMT presence on the electrode [31]. Accordingly more gold nanoparticles were strongly bonded via its thiol groups to the surface of AMT-PGE due to the affinity of gold to thiol groups [32][33][34][35][36].…”

Section: Resultsmentioning

confidence: 99%

“…The electrodes were pretreated before AMT immobilization and 3000 µg/mL AMT was used for full coverage of the electrode surface according to our previous study [31]. AuNP dilution ratio was studied to investigate the convenient quantity of AuNP at the modified AMT-PGE surface.…”

Section: Resultsmentioning

confidence: 99%

“…PGEs were first electrochemically pretreated in ABS by applying +1.40 V for 30 s. Each pretreated pencil lead was then immersed into the vials containing 110 µL of 3000 µg/mL freshly prepared AMT solution for an hour to make an AMT layer on the electrode surface [31]. Each of this AMT modified PGEs were placed at upside down position in dark for 30 min to allowed to dry.…”

Section: Procedures Preparation Of Aunp-amt-pge and Thiol Linked Probementioning

confidence: 99%

“…In addition, thiol groups provide strong and dense attachment to the gold nanoparticles. These gold nanoparticles provide also more and strong immobilization of thiol labeled DNA onto the electrode surface as a result of the specific interaction [31].…”

Section: Procedures Preparation Of Aunp-amt-pge and Thiol Linked Probementioning

confidence: 99%

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Impedimetric Nanobiosensor for the Detection of Sequence-Selective DNA Hybridization

Muti¹,

Muti²,

Erdem³

2018

HJBC

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A ltın nanopartiküller (AuNPs) ile zenginleştirilmiş 5-amino-2-mercapto-1,3,4-thidiazole (AMT) modifiye impedimetrik sensörler, Hepatit B virüsüne (HBV) ilişkin dizi seçimli DNA hibridizasyonunun elektrokimyasal olarak izlenmesi için geliştirilmiştir. AMT-AuNP-PGE ler daha fazla miktarda DNA'nın AMT-AuNPs-PGE yüzeyine bağlanması için uygun bir yüzey sağlamış ve daha tekrarlanabilir impedimetrik sinyaller vermiştir. AMT-AuNP ile modifiye edilmiş DNA biyosensörlerin seçimliliği, hedef DNA, veya diğer DNA dizileri; rastgele dizi (NC), mutasyonlu dizi (MM) varlığında test edilmiştir. Tayin sınırı 86 µg/mL olarak hesaplanmıştır. Anahtar KelimelerAltın nanopartiküller, 5-amino-2-mercapto-1,3,4-thidiazole, DNA hibridizasyonu, impedimetrik sensor. A B S T R A C T5 -amino-2-mercapto-1,3,4-thidiazole (AMT) enriched gold nanoparticles (AuNPs) modified impedimetric sensors were developed for the electrochemical monitoring of sequence-selective DNA hybridization related to Hepatitis B virus (HBV). AMT-AuNP-PGEs presented more repeatable impedimetric responses and provided a suitable surface for more DNA binding onto AMT-AuNPs-PGE surface. The selectivity of DNA biosensor modified with AMT-AuNP was investigated in the presence of target DNA, or the other DNA sequences; e.g, noncomplementary (NC), or mismatch (MM) DNA sequences. The detection limit was calculated as 0.86 µg/mL.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Procedures Preparation Of Aunp-amt-pge and Thiol Linked Probementioning

confidence: 99%

Section: Procedures Preparation Of Aunp-amt-pge and Thiol Linked Probementioning

confidence: 99%

See 2 more Smart Citations

Impedimetric Nanobiosensor for the Detection of Sequence-Selective DNA Hybridization

Muti¹,

Muti²,

Erdem³

2018

HJBC

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“…The most important objective in DNA biosensors is to fabricate inexpensive, reproducible and rapid‐response devices for detection of DNA hybridization with high specificity and sensitivity. There are many studies based on spectrometry , surface plasmon resonance and electrochemical techniques which have been made to DNA determination. Electrochemical techniques bring some advantages such as simplicity, high speed, low cost, and high sensitivity.…”

Section: Introductionmentioning

confidence: 99%

Electrochemical Determination of Label Free BRCA Hybridization by Single Use Antioxidant Modified Electrode

Muti

2017

Electroanalysis

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A novel DNA probe based on caffeic acid modified disposable pencil graphite electrodes were developed for the first time for the electrochemical determination of breast cancer gene sequence (BRCA) hybridization. Amino‐linked BRCA probe highly immobilized onto the caffeic acid modified electrode by means of the interaction between the amino group of BRCA probe and the carboxyl group of caffeic acid compared to the bare electrode. 44 % signal enhancement in guanine oxidation signal was measured by caffeic acid modified electrode. Besides, these probes exhibited high selectivity towards its complementary DNA sequences (target). Hybridization between probe and target (BRCA1) was studied to evaluate the selectivity of the probes for complementary, non‐complementary and mismatch sequences. The selectivity was also tested in the presence of mixture containing the target and one base mismatch BRCA sequences in the same ratio (1 : 1). It can be said this probe can select its complementary from the mixture.

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A Novel DNA Probe Based on Molecularly Imprinted Polymer Modified Electrode for the Electrochemical Monitoring of DNA

et al. 2015

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A DNA probe that was based on methylene blue (MB) imprinted polyvinyl pyridine polymer (MIP) modified carbon paste electrodes were developed for the first time for electrochemical monitoring of DNA. Probes were built up by adsorbing MB onto modified electrodes prior to DNA immobilization. It was shown that DNA strongly immobilizes on MIP modified electrodes when MB was adsorbed in advance of DNA immobilization. The performance of the MB imprinted polymer modified carbon paste electrodes (MIP‐CPE) to rebind the template molecule (MB) were compared to those of control polymer modified (non‐imprinted polymer NIP‐CPE) and bare (CPE) electrodes. Electrochemical signal resulting from the oxidation of guanine moiety of the immobilized probe DNA was high enough on the constructed platform, implicating that probes of this kind could be favorably used for DNA analysis. These probes exhibited high selectivity for its complementary DNA sequences (target). HBV‐DNA hybridization was studied to evaluate the selectivity of the probes for complementary, non‐complementary and mismatch sequences. The detection limit of the probe for the target DNA was 8.72 µg/mL (1.38 µM), which was better than those attained by some earlier DNA sensor studies.

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5-Amino-2-mercapto-1,3,4-thidiazole modified single-use sensors for electrochemical DNA analysis

Cited by 9 publications

References 22 publications

Impedimetric Nanobiosensor for the Detection of Sequence-Selective DNA Hybridization

Impedimetric Nanobiosensor for the Detection of Sequence-Selective DNA Hybridization

Electrochemical Determination of Label Free BRCA Hybridization by Single Use Antioxidant Modified Electrode

A Novel DNA Probe Based on Molecularly Imprinted Polymer Modified Electrode for the Electrochemical Monitoring of DNA

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