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Lewenza, Shawn; Hancock, Robert E. W.

doi:10.1186/gb-2001-3-1-reports4002

Cited by 3 publications

(6 citation statements)

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“…Alginate levels, ␤-galactosidase, and alkaline phosphatase activity were measured by using the established methods as described in SI Text (34,35).…”

Section: Methodsmentioning

confidence: 99%

Regulated proteolysis controls mucoid conversion in Pseudomonas aeruginosa

Qiu¹,

Eisinger²,

Rowen

et al. 2007

Proc. Natl. Acad. Sci. U.S.A.

137

254

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Overproduction of the exopolysaccharide alginate causes mucoid conversion in Pseudomonas aeruginosa and is a poor prognosticator in cystic fibrosis. The ECF factor AlgU and its cognate antifactor MucA are two principal regulators of alginate production. Here, we report the identification of three positive regulators of alginate biosynthesis: PA4033 (designated mucE), PA3649 (designated mucP), and algW. MucE, a small protein (9.5 kDa), was identified as part of a global mariner transposon screen for new regulators of alginate production. A transposon located in its promoter caused the overexpression of MucE and mucoid conversion in P. aeruginosa strains PAO1 and PA14. Accumulation of MucE in the envelope resulted in increased AlgU activity and reduced MucA levels. Three critical amino acid residues at the C terminus of MucE (WVF) were required for mucoid conversion via two predicted proteases AlgW (DegS) and MucP (RseP/YaeL). Moreover, as in Escherichia coli, the PDZ domain of AlgW was required for signal transduction. These results suggest that AlgU is regulated similarly to E. coli E except that the amino acid triad signals from MucE and other envelope proteins that activate AlgW are slightly different from those activating DegS.alginate ͉ anti-factor ͉ PDZ domain ͉ protease cascade ͉ signal specificity

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“…Alginate levels, ␤-galactosidase, and alkaline phosphatase activity were measured by using the established methods as described in SI Text (34,35).…”

Section: Methodsmentioning

confidence: 99%

Regulated proteolysis controls mucoid conversion in Pseudomonas aeruginosa

Qiu¹,

Eisinger²,

Rowen

et al. 2007

Proc. Natl. Acad. Sci. U.S.A.

137

254

View full text Add to dashboard Cite

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“…The chromophore is then cyclized by the tyrosinase PvdP, which converts the non-fluorescent ferribactin, into the fluorescent PVDI [29]. The periplasmic enzymes PvdN and PvdO [21,30] are also necessary for PVDI production [28,[31][32][33]: mutation of PvdN and PvdO abolishes the secretion of fluorescent PVDI [28]. However, the exact functions of these enzymes are still unknown.…”

Section: The Biochemistry Of the Pvdi Biosynthesis Pathway In P Aerumentioning

confidence: 99%

Cellular organization of siderophore biosynthesis in Pseudomonas aeruginosa: Evidence for siderosomes

Gasser

Guillon

Cunrath

et al. 2015

Journal of Inorganic Biochemistry

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“…[42] The calculation of LLE AT with Equations (5)-(8) was based on the work of Mortenson and Murray used for the calcula-tion pIC 50 -derived LLE AT values by Equations (9)-(11), published by Thomann et al [35,43] Synthesis and analytical characterization.N MR spectra were recorded on aB ruker Avance AV300 or aBruker DRX500. The residual 1 Ho r 13 Cr esonances of the > 99 %d euterated solvents were used for internal reference of all spectra acquired ( [2,16,43] Analytical and experimental details of intermediates can be found in the Supporting Information.…”

Section: Methodsmentioning

confidence: 99%

“…[8] Thism atrix consists of various components such as extracellularD NA (eDNA), polysaccharides, and proteins which have been shown to be important concealing the pathogen from host defense mechanisms [9] and antibiotics. [10] In particular, eDNA has recently been discussed as one major factor that prevents the antibiotic activity of aminoglycosides [11] and fluoroquinolones, [12,13] which are the first choice to be combined with b-lactam antibiotics in the treatment of PA infections. Moreover,e DNA is heavily involved in resistance againsta ntimicrobial peptides of the host'si nnate immune system.…”

Section: Introductionmentioning

confidence: 99%

“…Moreover,e DNA is heavily involved in resistance againsta ntimicrobial peptides of the host'si nnate immune system. [13,14] Therefore, agents that decreaseb iofilm and eDNA hold great potential not only to restore antibiotic efficacy,b ut also to enable the human immune systemt oc lear the infection. Biofilm formation and eDNA release are both controlled by cell-density-dependent communication systemsi nP A.…”

Section: Introductionmentioning

confidence: 99%

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Structure–Activity Relationships of 2‐Sufonylpyrimidines as Quorum‐Sensing Inhibitors to Tackle Biofilm Formation and eDNA Release of Pseudomonas aeruginosa

Thomann

Brengel

Börger³

et al. 2016

ChemMedChem

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Drug-resistant Pseudomonas aeruginosa (PA) strains are on the rise, making treatment with current antibiotics ineffective. Hence, circumventing resistance or restoring the activity of antibiotics by novel approaches is of high demand. Targeting the Pseudomonas quinolone signal quorum sensing (PQS-QS) system is an intriguing strategy to abolish PA pathogenicity without affecting the viability of the pathogen. Herein we report the structure-activity relationships of 2-sulfonylpyrimidines, which were previously identified as dual-target inhibitors of the PQS receptor PqsR and the PQS synthase PqsD. The SAR elucidation was guided by a combined approach using ligand efficiency and ligand lipophilicity efficiency to select the most promising compounds. In addition, the most effective inhibitors were rationally modified by the guidance of QSAR using Hansch analyses. Finally, these inhibitors showed the capacity to decrease biofilm mass and extracellular DNA, which are important determinants for antibiotic resistance.

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Cited by 3 publications

References 0 publications

Regulated proteolysis controls mucoid conversion in Pseudomonas aeruginosa

Regulated proteolysis controls mucoid conversion in Pseudomonas aeruginosa

Cellular organization of siderophore biosynthesis in Pseudomonas aeruginosa: Evidence for siderosomes

Structure–Activity Relationships of 2‐Sufonylpyrimidines as Quorum‐Sensing Inhibitors to Tackle Biofilm Formation and eDNA Release of Pseudomonas aeruginosa

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