Cellular organization of siderophore biosynthesis in Pseudomonas aeruginosa: Evidence for siderosomes

Gasser, Véronique; Guillon, Laurent; Cunrath, Olivier; Schalk, Isabelle J.

doi:10.1016/j.jinorgbio.2015.01.017

Cited by 54 publications

(66 citation statements)

References 64 publications

(100 reference statements)

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“…PvdRT and OpmQ mediate the transport of the newly synthesized pyoverdine across the outer membrane and the recycling of pyoverdines. The FvpA receptor is a TonB-transporter family protein involved in pyoverdine-Fe sensing and internalization (Imperi et al, 2009; Cornelis, 2010; Schalk and Guillon, 2013; Gasser et al, 2015; Chen et al, 2016). fpvI that encodes for a RNA polymerase ECF-type sigma factor is also present in all the strains at the same locus.…”

Section: Resultsmentioning

confidence: 99%

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Iron Uptake Analysis in a Set of Clinical Isolates of Pseudomonas putida

et al. 2016

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Pseudomonas putida strains are frequent inhabitants of soil and aquatic niches and they are occasionally isolated from hospital environments. As the available iron sources in human tissues, edaphic, and aquatic niches are different, we have analyzed iron-uptake related genes in different P. putida strains that were isolated from all these environments. We found that these isolates can be grouped into different clades according to the genetics of siderophore biosynthesis and recycling. The pyoverdine locus of the six P. putida clinical isolates that have so far been completely sequenced, are not closely related; three strains (P. putida HB13667, HB3267, and NBRC14164T) are grouped in Clade I and the other three in Clade II, suggesting possible different origins and evolution. In one clinical strain, P. putida HB4184, the production of siderophores is induced under high osmolarity conditions. The pyoverdine locus in this strain is closely related to that of strain P. putida HB001 which was isolated from sandy shore soil of the Yellow Sea in Korean marine sand, suggesting their possible origin, and evolution. The acquisition of two unique TonB-dependent transporters for xenosiderophore acquisition, similar to those existing in the opportunistic pathogen P. aeruginosa PAO, is an interesting adaptation trait of the clinical strain P. putida H8234 that may confer adaptive advantages under low iron availability conditions.

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Section: Resultsmentioning

confidence: 99%

“…The main mechanism for capturing iron is through high affinity of siderophores (Payne, 1980; Schalk and Guillon, 2013; Gasser et al, 2015). P. putida produces a main siderophore that has been previously identified as pyoverdine (Matthijs et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

Iron Uptake Analysis in a Set of Clinical Isolates of Pseudomonas putida

et al. 2016

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“…Cellular localization of the later biosynthetic steps awaits elucidation, including that of NRPSs, SidD, and SidC, although identification of both NRPSs from the microsomal fraction during iron starvation suggests that these proteins may either be localized at membranes or enclosed in membrane-bound compartments (Moloney et al, 2016). Such localization has been reported in Pseudomonas aeruginosa where the formation of “siderosomes” at membranes is postulated to increase biosynthetic efficiency (Imperi and Visca, 2013; Gasser et al, 2015). Recent work has also revealed the localization of siderophore procurement and processing mechanisms.…”

Section: Discussionmentioning

confidence: 58%

Functional Investigation of Iron-Responsive Microsomal Proteins, including MirC, in Aspergillus fumigatus

et al. 2017

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The functionality of many microsome-associated proteins which exhibit altered abundance in response to iron limitation in Aspergillus fumigatus is unknown. Here, we generate and characterize eight gene deletion strains, and of most significance reveal that MirC (AFUA_2G05730) contributes to the maintenance of intracellular siderophore [ferricrocin (FC)] levels, augments conidiation, confers protection against oxidative stress, exhibits an intracellular localization and contributes to fungal virulence in the Galleria mellonella animal model system. FC levels were unaffected following deletion of all other genes encoding microsome-associated proteins. MirC does not appear to play a role in either siderophore export from, or uptake into, A. fumigatus. Label-free quantitative proteomic analysis unexpectedly revealed increased abundance of siderophore biosynthetic enzymes. In addition, increased expression of hapX (7.2 and 13.8-fold at 48 and 72 h, respectively; p < 0.001) was observed in ΔmirC compared to wild-type under iron-replete conditions by qRT-PCR. This was complemented by significantly elevated extracellular triacetylfusarinine C (TAFC; p < 0.01) and fusarinine C (FSC; p < 0.05) siderophore secretion. We conclude that MirC plays an important role in FC biosynthesis and contributes to the maintenance of iron homeostasis in A. fumigatus.

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“…To increase Fe assimilation, organisms developed several strategies, and the most common is the production of extracellular siderophores (Rajkumar et al 2010;Sahu and Sindhu 2011). Siderophores are low molecular mass compounds with high iron affinity and are typically produced in secondary metabolism by bacteria, fungi, and monocotyledonous plants in response to Fe stress (Gasser et al 2015 +3 ), so in biological media, the selectivity to Fe +3 will be more effective (Hider and Kong 2010). There are basically four chemical classes of bacterial siderophores: phenol-catecholates, hydroxamates, rhizobactins, and hydroxycarboxylates.…”

Section: Nutrient Competition and Siderophore Productionmentioning

confidence: 99%

Use of Plant Growth-Promoting Rhizobacteria as Biocontrol Agents: Induced Systemic Resistance Against Biotic Stress in Plants

Salomón

Pinter

Píccoli

2017

Microbial Applications Vol.2

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Plant growth-promoting rhizobacteria (PGPR) are free-living bacteria able to colonize roots and soil around them that have a positive effect on plant growth, development, and health. One of the mechanisms by which PGPR exert a beneficial effect involves the capacity to control growth of deleterious organisms diminishing or preventing their negative effects on plant health and growth. Pathogen biocontrol implicates diverse features of bacteria; one of them is the antagonism that excludes pathogen due to the ability of some bacteria to colonize faster and more effectively a niche, reducing nutrient availability for the deleterious organism. Also some bacteria produce antibiotics, organic compounds that are lethal in low concentration for growth and metabolic activities of other microorganisms. Finally, the ability of bacteria to elicit a defense response in plant, called induced systemic resistance (IRS), involves the induction of synthesis of defense metabolites, but without causing a disease itself, enhancing the plant's defensive capacity. This chapter analyzed and discussed PGPR as biocontrol agent and the possibility to use them as ecological alternative to the use of agrochemicals, since they have been proved in different plant species in order to diminish the damage of pathogen and to reduce losses in crops.

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Cellular organization of siderophore biosynthesis in Pseudomonas aeruginosa: Evidence for siderosomes

Cited by 54 publications

References 64 publications

Iron Uptake Analysis in a Set of Clinical Isolates of Pseudomonas putida

Iron Uptake Analysis in a Set of Clinical Isolates of Pseudomonas putida

Functional Investigation of Iron-Responsive Microsomal Proteins, including MirC, in Aspergillus fumigatus

Use of Plant Growth-Promoting Rhizobacteria as Biocontrol Agents: Induced Systemic Resistance Against Biotic Stress in Plants

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