4D hydrogel for dynamic cell culture with orthogonal, wavelength-dependent mechanical and biochemical cues

Zheng, Yijun; Han, Mitchell K. L.; Jiang, Qiyang; Li, Bin; Feng, Jun; Campo, Aránzazu del

doi:10.1039/c9mh00665f

Cited by 40 publications

(32 citation statements)

References 43 publications

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“…The capability of PEGDA‐DTT waveguides to guide light and remotely photoactivate biological processes was tested in vitro. A waveguide was used to deliver 405 or 450 nm light through >5 cm porcine tissue to test two different scenarios ( Scheme ): i) from a tissue regeneration perspective, the possibility to remotely trigger migration of cells from spheroids to colonize a surrounding photoactivatable hydrogel, [ 34 ] and ii) from a therapeutic perspective, the ability to remotely induce the secretion of a drug from optogenetically‐engineered bacteria encapsulated in a hydrogel. [ 35 ]…”

Section: Resultsmentioning

confidence: 99%

Printed Degradable Optical Waveguides for Guiding Light into Tissue

Feng

Zheng

Bhusari

et al. 2020

Adv Funct Materials

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Optogenetics and photonic technologies are changing the future of medicine. To implement light-based therapies in the clinic, patient-friendly devices that can deliver light inside the body while offering tunable properties and compatibility with soft tissues are needed. Here extrusion printing of degradable, hydrogel-based optical waveguides with optical losses as low as 0.1 dB cm −1 at visible wavelengths is described. Core-only and core-cladding fibers are printed at room temperature from polyethylene glycol (PEG)-based and PEG/Pluronic precursors, and cured by in situ photopolymerization. The obtained waveguides are flexible, with mechanical properties tunable within a tissue-compatible range. Degradation times are also tunable by adjusting the molar mass of the diacrylate gel precursors, which are synthesized by linking PEG diacrylate (PEGDA) with varying proportions of DL-dithiothreitol (DTT). The printed waveguides are used to activate photochemical and optogenetic processes in close-to-physiological environments. Light-triggered migration of cells in a photoresponsive 3D hydrogel and drug release from an optogenetically-engineered living material by delivering light across >5 cm of muscle tissue are demonstrated. These results quantify the in vitro performance, and reflect the potential of the printed degradable fibers for in vivo and clinical applications.

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Section: Resultsmentioning

confidence: 99%

Printed Degradable Optical Waveguides for Guiding Light into Tissue

Feng

Zheng

Bhusari

et al. 2020

Adv Funct Materials

Self Cite

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“…With its unique properties, light can serve as a gate to control chemical bond formation and cleavage, which in turn permits precise control over both chemical and physical properties of materials 8–10. In the field of hydrogels, a number of studies have successfully developed light‐based methods to tune the mechanical properties of gels for their subsequent utilization in biomaterial engineering 11–14…”

Section: Introductionmentioning

confidence: 99%

“…Such systems were readily expanded by the incorporation of additional photolabile bonds, which allowed for a further light‐triggered response of the gel. The resulting two wavelength responsive gels enabled, in addition to the visible light‐induced stiffening, a UV light‐initiated softening of the gel12 or change in adhesive properties 14. Other systems exploited spontaneous recognition events such as host–guest15 and protein–protein interactions16 to form hydrogels, which can be modulated by a light‐induced conformation change of the guest or binding protein.…”

Section: Introductionmentioning

confidence: 99%

Wavelength‐Dependent Stiffening of Hydrogel Matrices via Redshifted [2+2] Photocycloadditions

Kalayci

Frisch

Barner‐Kowollik

et al. 2020

Adv Funct Materials

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Hydrogels with on-demand tunable mechanical properties within sensitive biological environments are of critical importance for examining cellular responses to cell culture platforms. Herein, the first bio-orthogonal hydrogel that can be formed and subsequently tuned in its mechanical properties by simply switching different wavelengths of visible light (i.e., 455 and 420 nm) is reported. Specifically, both the initial hydrogelation and the tuning of the mechanical properties can be fully decoupled and selectively initiated by different colors of light. Sparing the need for any catalysts, the development of such a dual wavelength selective hydrogel for biological applications spans four levels: First, the development of the until today most redshifted photocycloaddition to allow for the selective initiation of only one photoreaction; second, the investigation of its wavelength-dependent ligation efficiency; third, translation of the ligation chemistry into a hydrogelator, and fourth, establishing a biocompatible hydrogel platform for applications in biomaterials engineering including detachment of fibroblasts from 2D culture areas or primary 3D culture of human mesenchymal stem cells. The introduced platform technology enables the fabrication of a hydrogel of predefined mechanical properties exclusively with visible light.

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“…[ 93 ] Wavelength‐dependent patterning has also been employed to trigger stiffening of dextran–MA hydrogels with visible light, while UV light was used to photocleave DMNPB groups to activate an adhesive peptide at irradiated volumes. [ 94 ]…”

Section: Biochemical Patterning Of Hydrogel Substratesmentioning

confidence: 99%

Surface Patterning of Hydrogel Biomaterials to Probe and Direct Cell–Matrix Interactions

Munoz‐Robles

Kopyeva

DeForest

2020

Adv Materials Inter

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Due to their mechanical and structural similarity to native tissues, hydrogel biomaterials have gained tremendous popularity for applications in 3D tissue culture, therapeutic screening, disease modeling, and regenerative medicine. Recent advances in pre‐ and post‐synthetic processing have afforded anisotropic manipulation of the biochemical, mechanical, and topographical properties of biocompatible gels, increasingly in a dynamic and heterogeneous fashion that mimics natural processes in vivo. Herein, the current state of hydrogel surface patterning to investigate cellular interactions with the surrounding matrix is reviewed, both in techniques utilized and biological findings explored, and the perspective on proposed future directions for the field is offered.

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4D hydrogel for dynamic cell culture with orthogonal, wavelength-dependent mechanical and biochemical cues

Abstract: A 4D hydrogel allows user-defined stiffening of the cellular environment and presentation of bioadhesive cues in an orthogonal manner using light of different wavelengths.

Cited by 40 publications

References 43 publications

Printed Degradable Optical Waveguides for Guiding Light into Tissue

Printed Degradable Optical Waveguides for Guiding Light into Tissue

Wavelength‐Dependent Stiffening of Hydrogel Matrices via Redshifted [2+2] Photocycloadditions

Surface Patterning of Hydrogel Biomaterials to Probe and Direct Cell–Matrix Interactions

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