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Barišić, Karmela; Petrik, József; Rumora, Lada; Čepelak, Ivana; Grubišić, Tihana Žanić

doi:10.1007/s00204-002-0335-9

Cited by 15 publications

(1 citation statement)

References 35 publications

(39 reference statements)

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“…[3][4][5][6] Apoptosis is a physiological cell suicide program that is critical for the maintenance of healthy tissues. 7 Inducing apoptosis in tumor cells by delivering suicide gene encoded plasmids has been proved to be efficient for cancer therapy. In our previous works, delivering apoptosis-inducing genes such as VSVMP and survivin-T34A has been evaluated on several cancer models and desired therapeutic effects were achieved.…”

Section: Introductionmentioning

confidence: 99%

Delivery of modified mRNA encoding vesicular stomatitis virus matrix protein for colon cancer gene therapy

Men

Zhang

et al. 2018

RSC Adv.

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Plasmid DNA based gene delivery has been widely utilized among both pre-clinical and clinical gene therapy studies. However, therapeutic efficiency is usually limited by the size and potential immunestimulation issue of plasmid backbone. As an alternative form of genetic material, chemically modified messenger RNA (mRNA) provides a promising alternative to plasmid DNA. In this work, an in vitro transcription mRNA encoding vesicular stomatitis virus matrix protein (VSVMP) was delivered by a cationic liposome-protamine complex, resulting in high mRNA transporting and expression efficiency.The liposome-protamine complex delivered VSVMP mRNA strongly inhibits the growth of C26 tumor cells through inducing apoptosis, while obvious tumor regressions were achieved on both abdominal cavity metastatic and subcutaneous xenograft models in vivo with high safety. Our results also demonstrated that the liposome-protamine-mRNA complex was as potent as its plasmid DNA counterpart, showing strong potential in further colon cancer therapy.

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Section: Introductionmentioning

confidence: 99%

Delivery of modified mRNA encoding vesicular stomatitis virus matrix protein for colon cancer gene therapy

Men

Zhang

et al. 2018

RSC Adv.

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Toxicities induced in cultured human hepatocarcinoma cells exposed to ochratoxin A: Oxidative stress and apoptosis status

Golli-Bennour

Rodriguez-Enfedaque

Bouaziz

et al. 2009

J Biochem & Molecular Tox

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Ochratoxin A (OTA) is a mycotoxin currently detected in stored animal and human food supplies as well as in human sera worldwide. OTA has diverse toxicological effects; however, the most prominent one is the nephrotoxicity. The present investigation was conducted to determine the molecular aspects of OTA toxicity in cultured human hepatocellular carcinoma cells. With this aim, we have monitored the effects of OTA on (i) cell viability, (ii) heat shock protein expressions as a parameter of protective and adaptive response, (iii) oxidative damage, and (iv) cell death signaling pathway. Our results clearly showed that OTA treatment inhibits cell proliferation, downregulates Hsp 70 and Hsp 27 protein and mRNA levels, and did not induce a significant reactive oxygen species generation. We have also demonstrated a decrease in mitochondrial membrane potential, a cytochrome c release, and an activation of caspase 9 and caspase 3 in response to OTA exposure. Moreover, OTA activates p53 expression, while some of its transcriptional target genes (Bax, Bak, PUMA, and p21) were found to downregulate. According to these data, we concluded that OTA may exert an inhibitory action on the transcriptional process. Besides, oxidative damage is not a major contributor to OTA toxicity. This mycotoxin induces a mitochondrial and caspase-dependent apoptotic cell death, which seems to be mediated by p53 transcriptional independent activities.

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High‐Throughput Tag‐Sequencing Analysis of Early Events Induced by Ochratoxin A in HepG‐2 Cells

Zheng

Luo

et al. 2015

J Biochem & Molecular Tox

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Ochratoxin A (OTA) is produced by fungi of the species Aspergillus and Penicillium. OTA has displayed hepatotoxicity in mammals. Although recent studies have indicated that OTA influences liver function, little is known regarding its impact on differential early liver toxicity. In this study, we report high-throughput tag-sequencing (Tag-seq) analysis of the transcriptome using Solexa Analyzer platform after 4 h of OTA treatment on HepG-2 cells. The analyses of differentially expressed genes revealed the substantial changes. A total of 21,449 genes were identified and quantified, with 2726 displaying significantly altered expression levels. Expression level data were then integrated with a network of gene-gene interactions, and biological pathways to obtain a systems-level view of changes in the transcriptome that occur with OTA resistance. Our data suggest that OTA exposure leads to an imbalance in zinc finger expression and shed light on splicing factor and mitochondrial-based mechanisms.

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Cited by 15 publications

References 35 publications

Delivery of modified mRNA encoding vesicular stomatitis virus matrix protein for colon cancer gene therapy

Delivery of modified mRNA encoding vesicular stomatitis virus matrix protein for colon cancer gene therapy

Toxicities induced in cultured human hepatocarcinoma cells exposed to ochratoxin A: Oxidative stress and apoptosis status

High‐Throughput Tag‐Sequencing Analysis of Early Events Induced by Ochratoxin A in HepG‐2 Cells

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