497. An improved medium for lactobacilli

Briggs, Mary

doi:10.1017/s0022029900006671

Cited by 70 publications

(29 citation statements)

References 20 publications

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“…The bacterial strains used in this study are listed in Table 2 (Briggs, 1953;Partanen et al, 2001;Williams et al, 1947). Sunsoft Q-17S, which is made from coleseed in Japan and used as an emulsifier for food processing, was added to FG medium instead of Tween 80, since oleic acid and linoleic acid are the major components of this emulsifier.…”

Section: Methodsmentioning

confidence: 99%

Development of food grade media for the preparation of Lactobacillus plantarum starter culture

Sawatari

Hirano

Yokota

2006

J. Gen. Appl. Microbiol.

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Based on MRS medium, two types of food grade (FG) culture media (FG medium I and FG medium II) for the preparation of a concentrated starter culture of Lactobacillus plantarum NRIC 0380 to manufacture a new type of instant Chinese noodle, the fermented instant Chinese noodle, were developed using FG materials. FG medium I, which is for normal static culture, contains table sugar (sucrose), Yeast peptone standard type F, Sunsoft Q-17S (emulsifier), sodium acetate, trisodium citrate and MnSO 4 · 4-5H 2 O. FG medium II was designed to be used for the pHcontrolled jar fermentor culture conditions. Therefore, sodium acetate and trisodium citrate as a buffer to prevent acidification of medium were omitted from FG medium I. When L. plantarum NRIC 0380 was cultured under the pH-controlled jar fermentor culture conditions, the kinetics of growth, sugar consumption and lactic acid production in FG medium II were quite similar to those observed in the Difco Lactobacilli MRS Broth. Furthermore, growths of many lactobacilli strains isolated from various fermented foods in FG medium I were also quite similar to those observed in MRS medium. Therefore, simple and practical FG media for the culture of lactobacilli were successfully established.

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Section: Methodsmentioning

confidence: 99%

Development of food grade media for the preparation of Lactobacillus plantarum starter culture

Sawatari

Hirano

Yokota

2006

J. Gen. Appl. Microbiol.

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“…This was a medium with low pH and containing vancomycin to inhibit the growth of other gram-positive bacteria such as enterococci. In initial studies, three other selective media were also tested: de Man, Rogosa, and Sharpe agar (MRS) (12); Rogosa agar (38); and tomato juice agar (10). For growth of gram-negative anaerobes, Wilkins-Chalgren anaerobe agar (44) was used with a gram-negative anaerobe-selective supplement containing vancomycin and nalidixic acid (final concentrations, 10 and 2.5 g/ml of medium, respectively; Oxoid Ltd.).…”

Section: Methodsmentioning

confidence: 99%

Identification of Equine Cecal Bacteria Producing Amines in an In Vitro Model of Carbohydrate Overload

Bailey¹,

Baillon²,

Rycroft

et al. 2003

Appl Environ Microbiol

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Acute laminitis has been associated with the overgrowth of gram-positive bacteria within the equine hindgut, causing the release of factor(s) leading to ischemia-reperfusion of the digits. The products of fermentation which trigger acute laminitis are, as yet, unknown; however, vasoactive amines are possible candidates. The objectives of this study were to use an in vitro model of carbohydrate overload to study the change in populations of cecal streptococci and lactobacilli and to establish whether certain species of these bacteria were capable of producing vasoactive amines from amino acids. Cecal contents from 10 horses were divided into aliquots and incubated anaerobically with either corn starch or inulin (fructan; both at 1 g/100 ml). Samples were taken at 6-h intervals over a 24-h period for enumeration of streptococci, lactobacilli, and gram-negative anaerobes by a dilution method onto standard selective growth media. The effects of the antibiotic virginiamycin (1 mg/100 ml) and calcium hydrogen phosphate (CaHPO 4 ; 0.3 g/100 ml) were also examined. Fermentation of excess carbohydrate was associated with increases in numbers of streptococci and lactobacilli (2-to 3.5-log unit increases; inhibited by virginiamycin) but numbers of gram-negative anaerobes were not significantly affected. A screening agar technique followed by 16S rRNA gene sequence analysis enabled the identification of 26 different bacterial strains capable of producing one or more vasoactive amines. These included members of the species Streptococcus bovis and five different Lactobacillus spp. These data suggest that certain bacteria, whose overgrowth is associated with carbohydrate fermentation, are capable of producing vasoactive amines which may play a role in the pathogenesis of acute laminitis.Fermentation of carbohydrate in the hindgut has been recognized as one of the primary events associated with acute laminitis in the horse (14). The ability of the equine small intestine to digest starch may be overwhelmed if starch is present in excess (Ͼ0.4% body weight [35]), leading to its metabolism by cecal and colonic bacteria and the production of lactic acid. Fructans, the oligosaccharides present in grass which act as the storage form of carbohydrate, do not appear to be digested by the small intestine (21) and so are also substrates for fermentative bacteria. The variation of fructan levels in grasses under different climatic conditions has been suggested to account for the seasonal incidence of acute laminitis (A. C. Longland, A. J. Cairns, and M. O. Humphreys, Proc. 16th Equine Nutr. Physiol. Soc., p. 258-259, 1999).Gram-positive bacteria have been implicated in the pathogenesis of laminitis in the carbohydrate overload model since they have been shown to overgrow in the cecum of horses given corn starch by stomach tube (16). This overgrowth, particularly of streptococci and lactobacilli, was associated with lactic acid accumulation and the death of gram-negative bacteria leading to endotoxin release, both of which were postulated t...

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“…Preparation of inocula. Pure cultures of lactobacilli in Briggs broth [1] were incubated at 37 C for 24 hr. Cultures of the other bacteria were grown at 37 C for 24 hr in the EGL medium in which the agar content was reduced to 0.1% and horse blood omitted.…”

Section: Methodsmentioning

confidence: 99%

Specific Establishment of Lactobacilli in the Digestive Tract of Germ‐Free Chickens

Morishita

Mitsuoka

Kaneuchi

et al. 1971

Japanese Journal of Microbiology

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Establishment of lactobacilli in the digestive tract of germ-free chicks was studied using the organisms isolated from chickens, human infants and sour milk and those received from the American Type Culture Collection. In the case of monocontamination, intestinal lactobacilli such as Lactobacillus acidophilus, L. salivarius and L. It was shown that lactobacilli were predominant in the digestive tract of chickens [11,15], and the lactobacillus flora was composed mainly of Lactobacillus acidophilus, L. salivarius and L. fermenti but rarely included other species [7]. Furthermore, the biotype of L. acidophilus from the chicken and the human showed a considerable difference [7].In the present studies, monocontamination and dicontamination of germ-free chicks with bacteria were attempted to shed light on the problems of why Lactobacillus species such as L. helveticus, L. jugurti, L. brevis, L. plantarum and L. casei are absent from the gut of chickens [7] and why the biotype I of L. acidophilus is rarely found in the gut of chickens [7]. It was expected that the monocontamination would determine whether or not chickens reject the colonization of the above species and L. acidophilus biotype I, and the dicontamination would determine whether or not an antagonistic activity of the indigenous microflora in the gut of chickens result in 1 Present address:

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497. An improved medium for lactobacilli

Cited by 70 publications

References 20 publications

Development of food grade media for the preparation of Lactobacillus plantarum starter culture

Development of food grade media for the preparation of Lactobacillus plantarum starter culture

Identification of Equine Cecal Bacteria Producing Amines in an In Vitro Model of Carbohydrate Overload

Specific Establishment of Lactobacilli in the Digestive Tract of Germ‐Free Chickens

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