[49] Rapid DNA isolations for enzymatic and hybridization analysis

Davis, Ronald W.; Thomas, Marjorie; Cameron, John; John, Thomas P. St.; Scherer, Stewart; Padgett, Richard A.

doi:10.1016/s0076-6879(80)65051-4

Cited by 560 publications

(201 citation statements)

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“…Yeasts total DNA was prepared by the rapid procedure of Davis et al 12 ) Plasmid DNA of E. coli was extracted according to Maniatis et al 13 ) Direct selection for G418 resistant transformants of s. cerevisiae…”

Section: Resultsmentioning

confidence: 99%

Transformation of the Yeast,Saccharomyces carlsbergensis, Using an Antibiotic Resistance Marker

Sakai

Yamamoto

1986

Agricultural and Biological Chemistry

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Section: Resultsmentioning

confidence: 99%

Transformation of the Yeast,Saccharomyces carlsbergensis, Using an Antibiotic Resistance Marker

Sakai

Yamamoto

1986

Agricultural and Biological Chemistry

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“…[17][18][19] DNA extraction Genomic DNA from whole blood containing EDTA was extracted by standard techniques. 20 Generic HLA-DR typing Generic DR typing was performed by PCR-SSO reverse dot blot using the Amplicor Kit (Hoffmann La Roche, Basel, Switzerland).…”

Section: Samplementioning

confidence: 99%

Lack of association between the polymorphism at the heat-shock protein (HSP70-2) gene and systemic lupus erythematosus (SLE) in the Mexican Mestizo population

et al. 2000

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Major histocompatibility complex (MHC) alleles have been recognized as genetic factors for developing systemic lupus erythematosus (SLE). In the present study we analyzed whether a heat-shock protein gene (HSP70-2) is involved in determining susceptibility to develop SLE in a Mexican Mestizo population. A HSP70-2 Pst I polymorphism was detected by a restriction fragment length polymorphism analysis of polymerase chain reaction (PCR-RFLP) in 107 SLE patients and 158 healthy controls. No statistically significant differences were observed in the HSP70-2 allele distribution between patients and healthy controls. HLA-DR analysis showed an increased frequency of HLA-DR3 allele in the patients group (P Ͻ 0.05, OR = 2.26, EF = 6.0%). On the other hand, when we analyzed HSP70-2 polymorphism in relation to HLA-DR3 allele, we could only detect an increased frequency of AB genotype in the DR3 negative patients (pC Ͻ 0.05, RR = 2.6, EF = 11.3%). Linkage disequilibrium was observed for three haplotypes: HLA-DR3- HSP70

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“…DNA from YAC clones representing the region between CBR and ERG (21,22) was made as described (23). Sizes of the YACs were determined by hybridization of Southern blots of pulsed field gels to human Cot\ DNA.…”

Section: Yac Clonesmentioning

confidence: 99%