[48] High-pressure liquid chromatography analysis of ozone-induced depletion of hydrophilic and lipophilic antioxidants in murine skin

Weber, Stefan; Jothi, Sumana; Thiele, Jens J.

doi:10.1016/s0076-6879(00)19050-0

Cited by 8 publications

(7 citation statements)

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“…High-performance liquid chromatography (HPLC) was performed using a Gynkotek HPLC system (Dionex-Softron GmbH, Germering, Germany) as described [24,65]. The HPLC system included an auto sampler (Gina 50), pump (M480G), degasser, and an electrochemical detector (ANTEC, Leiden, The Netherlands).…”

Section: Vitamin E Analysismentioning

confidence: 99%

Low serum α‐tocopherol and selenium are associated with accelerated apoptosis in severe sepsis

et al. 2008

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During sepsis, a severe systemic disorder, micronutrients often are decreased. Apoptosis is regarded as an important mechanism in the development of often significant immunosuppression in the course of the disease. This study aimed to investigate alpha-tocopherol and selenium in reference to apoptosis in patients with sepsis. 16 patients were enrolled as soon as they fulfilled the criteria of severe sepsis. 10 intensive care patients without sepsis and 11 healthy volunteers served as controls. alpha-Tocopherol, selenium and nucleosomes were measured in serum. Phosphatidylserine externalization and Bcl-2 expression were analyzed in T-cells by flow cytometry. Serum alpha-tocopherol and selenium were decreased in severe sepsis but not in non-septic critically ill patients (p < 0.05). Conversely, markers of apoptosis were increased in sepsis but not in critically ill control patients: Nucleosomes were found to be elevated 3 fold in serum (p < 0.05) and phosphatidylserine was externalized on an expanded subpopulation of T-cells (p < 0.05) while Bcl-2 was expressed at lower levels (p < 0.05). The decrease of micronutrients correlated with markers of accelerated apoptosis. Accelerated apoptosis in sepsis is associated with low alpha-tocopherol and selenium. The results support the investigation of micronutrient supplementation strategies in severe sepsis.

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Section: Vitamin E Analysismentioning

confidence: 99%

Low serum α‐tocopherol and selenium are associated with accelerated apoptosis in severe sepsis

et al. 2008

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“…Cys, ␥-EC, GSH, and PC levels in the seedlings obtained from experiment B were analyzed by HPLC according to the method of Weber et al (1999). NPTs were extracted from 100 mg fresh weight of both shoot and root samples using 200 L of 10% sulfosalicylic acid containing 12.6 mm diethylenetriaminepentaacetic acid.…”

Section: Glutathione and Npt Analysismentioning

confidence: 99%

Cadmium Tolerance and Accumulation in Indian Mustard Is Enhanced by Overexpressing γ-Glutamylcysteine Synthetase

et al. 1999

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To investigate rate-limiting factors for glutathione and phytochelatin (PC) production and the importance of these compounds for heavy metal tolerance, Indian mustard (Brassica juncea) was genetically engineered to overexpress the Escherichia coli gshI gene encoding ␥-glutamylcysteine synthetase (␥-ECS), targeted to the plastids. The ␥-ECS transgenic seedlings showed increased tolerance to Cd and had higher concentrations of PCs, ␥-GluCys, glutathione, and total non-protein thiols compared with wild-type (WT) seedlings. When tested in a hydroponic system, ␥-ECS mature plants accumulated more Cd than WT plants: shoot Cd concentrations were 40% to 90% higher. In spite of their higher tissue Cd concentration, the ␥-ECS plants grew better in the presence of Cd than WT. We conclude that overexpression of ␥-ECS increases biosynthesis of glutathione and PCs, which in turn enhances Cd tolerance and accumulation. Thus, overexpression of ␥-ECS appears to be a promising strategy for the production of plants with superior heavy metal phytoremediation capacity.Heavy metals and metalloids such as Cd, Pb, Hg, As, and Se are an increasing environmental problem worldwide. Plants can be used to remove heavy metals by accumulating, stabilizing, or biochemically transforming them. This cost-effective and environment-friendly technology has been called "phytoremediation" (Salt et al., 1995). Hyperaccumulators-plant species that accumulate extremely high concentrations of heavy metals in shoots-offer one option for the phytoremediation of metal-contaminated sites. However, hyperaccumulators tend to grow slower and produce little biomass (Brooks, 1994). An alternative approach is to genetically engineer fast-growing species to improve their metal tolerance and metal-accumulating capacity. A suitable target species for this strategy is Indian mustard (Brassica juncea), which has a large biomass production, a relatively high trace element accumulation capacity (Dushenkov et al., 1995), and can be genetically engineered .Non-protein thiols (NPTs), which contain a high percentage of Cys sulfhydryl residues in plants, play a pivotal role in heavy metal detoxification. The reduced form of glutathione (␥-Glu-Cys-Gly, GSH) is one of the most important components of NPT metabolism. GSH may play several roles in heavy metal tolerance and sequestration. It protects cells from oxidative stress damage, such as that caused by heavy metals in plants (Gallego et al

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“…They also demonstrated that the decrease of endogenous α-tocopherol was found only in the upper epidermis, whereas the lower layers remained unaltered 32 . Similar studies on hairless mice showed that other endogenous skin antioxidants such as glutathione, uric acid and vitamin C are also sensitive to depletion when exposed to high and acute O3 concentrations (1 and 10 ppm for 2h) 44,45 . In addition, the O3 oxidative power within SC on other targets (such as proteins and lipids) was also confirmed 32 .…”

Section: In Vivo Murine Studiesmentioning

confidence: 64%

“…• 2 ppm twice per day for 7 days 97 • 1 ppm for 2h 45 • 8 ppm for 2h 46 • 0.8 ppm for 6h 50 • 0.5 ppm for 6h/day for 9 days 51* • 0.25 ppm for 6h/day for 4 days 52…”

Section: Conflict Of Interestmentioning

confidence: 99%

Bench approaches to study the detrimental cutaneous impact of tropospheric ozone

Petracca

Rothen‐Rutishauser

Valacchi

et al. 2020

J Expo Sci Environ Epidemiol

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Being exposed to ground-level ozone (O3), as it is often the case in polluted cities, is known to have a detrimental impact on skin. O3 induces antioxidant depletion and lipid peroxidation in the upper skin layers and this effect has repercussions on deeper cellular layers, triggering a cascade of cellular stress and inflammatory responses. Repetitive exposure to high levels of O3 may lead to chronic damages of the cutaneous tissue, cause premature skin aging and aggravate skin diseases such as contact dermatitis and urticaria. This review paper debates about the most relevant experimental approaches that must be considered to gather deeper insights about the complex biological processes that are activated when the skin is exposed to O3. Having a better understanding of O3 effects on skin barrier properties and stress responses could help the whole dermato-cosmetic industry to design innovative protective solutions and develop specific cosmetic regime to protect the skin of every citizen, especially those living in areas where exposure to high levels of O3 is of concern to human health.

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[48] High-pressure liquid chromatography analysis of ozone-induced depletion of hydrophilic and lipophilic antioxidants in murine skin

Cited by 8 publications

References 8 publications

Low serum α‐tocopherol and selenium are associated with accelerated apoptosis in severe sepsis

Low serum α‐tocopherol and selenium are associated with accelerated apoptosis in severe sepsis

Cadmium Tolerance and Accumulation in Indian Mustard Is Enhanced by Overexpressing γ-Glutamylcysteine Synthetase

Bench approaches to study the detrimental cutaneous impact of tropospheric ozone

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