47—the Microbial Degumming of Raw Ramie Fibre

Paul, Nilendu; Bhattacharyya, S. K.

doi:10.1080/00405007908658895

Cited by 22 publications

(7 citation statements)

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“…Suitable dilutions of the supernatant (1 ml) were added to 5 ml of PGA (polygalacturonic acid, sodium salt) solution (0.75%, w/v). The assay volumes were made up to 10.0 ml with Tris-HCl buVer (25 mM, pH 8.5) containing 1 mM CaCl 2 and incubated at 75°C for 2 h. About 0.6 ml zinc sulphate (9.0%, w/v) and 0.6 ml sodium hydroxide (0.5 M) were then added. The samples were centrifuged (3,000£g, 10 min) and 5.0 ml of the clear supernatant was added to a mixture of thiobarbituric acid (3.0 ml, 0.04 M) and HCl (1.5 ml, 0.1 M).…”

Section: Culture Conditions For Degumming On Small Scalementioning

confidence: 99%

“…The gummy material could also be removed by polysaccharidedegrading microorganisms or their enzymes in a biochemical degumming process. A combination of microbial and chemical processes has been proposed to reduce the consumption of chemicals and energy [10,11]. In this work, we report the degumming of the ramie bast Wbre by using a newly isolated pectinolytic Bacillus pumilus DKS1 strain and the characterisation of the degummed ramie Wbre.…”

Section: Introductionmentioning

confidence: 99%

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Large-scale degumming of ramie fibre using a newly isolated Bacillus pumilus DKS1 with high pectate lyase activity

Basu¹,

Saha²,

Chattopadhyay³

et al. 2008

J Ind Microbiol Biotechnol

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A combined (enzymatic and chemical) process using a Bacillus pumilus strain (DKS1), isolated from the soil, was used to degum ramie bast fibres. After 24 h of incubation with the isolated pectinolytic strain using a low-cost medium, the weight loss of the ramie fibre was found to be 25% under small scale. High activity of pectate lyase was detected in the culture supernatants; 400 kg of ramie fibres was degummed with 24% weight loss in large-scale degumming under field conditions. No cellulase activity was found. Microbial intervention followed by mild (0.1%) alkali treatment showed high percentage of weight loss from the ramie fibre. Bacterial degumming followed by chemical treatment resulted in an increase of single fibre tenacity (cN/tex) by more than 20.81% as compared to non-degummed (decorticated) fibre samples. Scanning electron micrographs (SEM) and fluorescence microscope showed that after Bacillus pumilus DKS1 treatment the surface of the decorticated ramie fibre becomes very smooth. These results indicate the process provides an economical and eco-friendly method for the small scale as well as large-scale degumming of decorticated ramie fibre. This study has great relevance to the textile as well as paper industry.

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Section: Culture Conditions For Degumming On Small Scalementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Large-scale degumming of ramie fibre using a newly isolated Bacillus pumilus DKS1 with high pectate lyase activity

Basu¹,

Saha²,

Chattopadhyay³

et al. 2008

J Ind Microbiol Biotechnol

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“…The incrusting material could also be removed by polysaccharide-degrading microorganisms or their enzymes in a biotechnical degumming process. Combined t Present address: Department of Biotechnology, University of Aalborg, DK-9000 Aalborg, Denmark. microbial and chemical processes have been proposed to reduce the consumption of chemicals and energy (10,20).…”

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confidence: 99%

Pectinolytic Enzymes from Actinomycetes for the Degumming of Ramie Bast Fibers

Brühlmann

Kim

Zimmerman

et al. 1994

Appl Environ Microbiol

113

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Actinomycetes isolated from 10 different soil and compost samples were screened for production of pectinolytic enzyme activities when grown on pectin-containing solid and liquid media. Pectinolytic enzymes, detected by using plate diffusion tests with a medium containing ramie (Boehmeria nivea) plant material as the sole carbon source, were mainly pectate lyases, but low activities of pectinesterases were also observed. Polygalacturonases and polymethylgalacturonases were not produced. Multiple forms of pectate lyases were detected in the culture supernatants of some of the strains by using the zymogram technique of isoelectric focusing gels. Xylanolytic and cellulolytic activities were always found to be associated with pectinolytic activities. None of the pectinolytic enzymes were produced in a medium with glucose as the sole carbon source. Treatment of ramie bast fibers with crude enzyme preparations from a selection of strains showed a good correlation between the pectate lyase activity applied and the degumming effect, resulting in good separation of the bast fibers.

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“…Traditional degumming methods are water retting and chemical boiling, which seriously pollute the environment and severely damage the fiber (Bhattacharyya and Paul, 1976;Gupta et al, 1976). Since 1954, scientists have done researches in biological ramie-degumming and enzymic degumming method (Paul and Bhattacharya, 1979). For bio-procession can override the defects caused by traditional procession, bio-degumming is the general trend of the ramie process Zhou, 1978).…”

Section: Introductionmentioning

confidence: 99%

Diversity and characterization of ramie-degumming strains

Duan

Liu

Feng

et al. 2012

Sci. agric. (Piracicaba, Braz.)

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Ramie (Boehmeria nivea and Boehmeria tenacissima) is a widely used fiber crop.Traditional water retting or chemical boiling method performed in order to extract ramie fiber seriously pollute the environment and severely damage the fiber, so biological method is the general trend of the fiber-extracting industry. Some strains (687), involving 26 genera and 43 species, were collected from the three samples, which produce hydrolyzed circles in the selective culture medium in order to detect the degumming effect and to compare the enzyme activity.Among these strains, 13 of them did not produce cellulase and had a ramie decreasing weight rate above 25 %, which were regarded as efficient ramie-degumming strains named from R1 Rhizobium sp. and Pseudomonas sp. were firstly reported to be used in ramie-degumming. At the same time, the pectinase was the key enzyme in the ramie-degumming process.

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47—the Microbial Degumming of Raw Ramie Fibre

Cited by 22 publications

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Large-scale degumming of ramie fibre using a newly isolated Bacillus pumilus DKS1 with high pectate lyase activity

Large-scale degumming of ramie fibre using a newly isolated Bacillus pumilus DKS1 with high pectate lyase activity

Pectinolytic Enzymes from Actinomycetes for the Degumming of Ramie Bast Fibers

Diversity and characterization of ramie-degumming strains

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