454 Pyrosequencing of Olive (Olea europaea L.) Transcriptome in Response to Salinity

Abstract

Olive (Olea europaea L.) is one of the most important crops in the Mediterranean region. The expansion of cultivation in areas irrigated with low quality and saline water has negative effects on growth and productivity however the investigation of the molecular basis of salt tolerance in olive trees has been only recently initiated. To this end, we investigated the molecular response of cultivar Kalamon to salinity stress using next-generation sequencing technology to explore the transcriptome profile of olive leaves and roots and identify differentially expressed genes that are related to salt tolerance response. Out of 291,958 obtained trimmed reads, 28,270 unique transcripts were identified of which 35% are annotated, a percentage that is comparable to similar reports on non-model plants. Among the 1,624 clusters in roots that comprise more than one read, 24 were differentially expressed comprising 9 down- and 15 up-regulated genes. Respectively, inleaves, among the 2,642 clusters, 70 were identified as differentially expressed, with 14 down- and 56 up-regulated genes. Using next-generation sequencing technology we were able to identify salt-response-related transcripts. Furthermore we provide an annotated transcriptome of olive as well as expression data, which are both significant tools for further molecular studies in olive.

Gaining Insight into Exclusive and Common Transcriptomic Features Linked to Drought and Salinity Responses across Fruit Tree Crops

“…Five genes, involved in hormone signaling, were up-regulated: ATB11 and ATP-dependent permease (PDR12) (ABA), germination insensitive to ABA mutant 2 (GIM2) (gibberellins), acetyl serotonin O -methyltransferase (ASMT) (salicylic acid), and SPCL51 (brassinosteroids). There is previous evidence that these genes are involved in drought or salinity tolerance/resistance [ 43 , 44 , 45 ]. PDR12 is a PDR-type ABC transporter that mediates cellular uptake of abscisic acid, and mutant experiments demonstrated that this gene facilitates stomata closure and enhances drought tolerance [ 44 ].…”

Recent agriculture adaption regarding climate change

“…Kalamon led to the identification of 387 unique ion transporter transcripts including 9 Na + transporters, 30 K + transporters and several other transcripts related to proton transport mechanism. 75…”

Growth of Different Olive Varieties Influences with Foliar Application of GA3 under Saline Conditions

“…Salinity is an important factor affecting plant productivity and constitutes a problem concerning a significant portion of the planet, especially in regions with hot, dry climates [22,23]. The availability of fresh water is one of the major limitations for crop production.…”