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Yan, Tsong‐Rong; Liau, Jian-Ching

doi:10.1023/a:1005362305545

Cited by 12 publications

References 9 publications

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Direct conversion of cellulose into ethanol and ethyl‐β‐d‐glucoside via engineered Saccharomyces cerevisiae

Jayakody

Liu

Yun

et al. 2018

Biotech & Bioengineering

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Simultaneous saccharification and fermentation (SSF) of cellulose via engineered Saccharomyces cerevisiae is a sustainable solution to valorize cellulose into fuels and chemicals. In this study, we demonstrate the feasibility of direct conversion of cellulose into ethanol and a biodegradable surfactant, ethyl-β-d-glucoside, via an engineered yeast strain (i.e., strain EJ2) expressing heterologous cellodextrin transporter (CDT-1) and intracellular β-glucosidase (GH1-1) originating from Neurospora crassa. We identified the formation of ethyl-β-d-glucoside in SSF of cellulose by the EJ2 strain owing to transglycosylation activity of GH1-1. The EJ2 strain coproduced 0.34 ± 0.03 g ethanol/g cellulose and 0.06 ± 0.00 g ethyl-β-d-glucoside/g cellulose at a rate of 0.30 ± 0.02 g·L ·h and 0.09 ± 01 g·L ·h , respectively, during the SSF of Avicel PH-101 cellulose, supplemented only with Celluclast 1.5 L. Herein, we report a possible coproduction of a value-added chemical (alkyl-glucosides) during SSF of cellulose exploiting the transglycosylation activity of GH1-1 in engineered S. cerevisiae. This coproduction could have a substantial effect on the overall technoeconomic feasibility of theSSF of cellulose.

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Direct conversion of cellulose into ethanol and ethyl‐β‐d‐glucoside via engineered Saccharomyces cerevisiae

Jayakody

Liu

Yun

et al. 2018

Biotech & Bioengineering

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Literaturverzeichnis

Lang¹,

Trowitzsch‐Kienast²

2002

Biotenside

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Enzymatic synthesis of alkyl glucosides using Leuconostoc mesenteroides dextransucrase

Kim

Ahn

et al. 2009

Biotechnol Lett

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Alkyl glucosides were synthesized by the reaction of Leuconostoc mesenteroides dextransucrase with sucrose and various alcohols. Alkyl alpha-D-glucosides were obtained with a yield of 30% (mol/mol) with primary alcohols, but secondary alcohols or tertiary alcohols gave yields below 5%. The optimal yield was 50% using 1-butyl alpha-D-glucoside with 0.9 M 1-butanol. The acceptor products of methanol or ethanol were confirmed as methyl alpha-D-glucopyranoside and ethyl alpha-D: -glucopyranoside via MALDI-TOF MS and NMR analysis. Thus, methyl or ethyl alpha-D-glucoside constituted half the emulsification activities of Triton X-100 as commercially available surfactants.

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Cited by 12 publications

References 9 publications

Direct conversion of cellulose into ethanol and ethyl‐β‐d‐glucoside via engineered Saccharomyces cerevisiae

Direct conversion of cellulose into ethanol and ethyl‐β‐d‐glucoside via engineered Saccharomyces cerevisiae

Literaturverzeichnis

Enzymatic synthesis of alkyl glucosides using Leuconostoc mesenteroides dextransucrase

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