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Abstract

These results are interpreted as indicating the formation of an amphotericin B-phospholipid complex, resulting in phase separation within the monolayer. The extent and nature of this phase separation was dependent on both the concentration of drug in the system, and the saturation state of the phospholipid component. The relevance of these observations to the stability of amphotericin B drug emulsions stabilised by saturated and unsaturated phospholipid emulsifiers is discussed. These observations may also be relevant to the toxicity of these, and other novel amphotericin B formulations.

Polyene antibiotic amphotericin B in monomolecular layers: spectrophotometric and scanning force microscopic analysis

“…1 presents isotherms of compression of the single monomolecular layers formed at the argon–water interface with pure AmB and a two‐component system composed of 90 mol% AmB and 10 mol% DPPC. The shape of the isotherm of compression of AmB is very close to the shape of isotherms previously reported [15,19–22]. The compression of AmB is a two‐phase process represented by two distinct linear portions of the isotherm of compression pointing to the specific molecular areas of 136 Å 2 and 36 Å 2 .…”

Surface Activities of a Lipid Analogue Room-Temperature Ionic Liquid and Its Effects on Phospholipid Membrane

“…The results suggest that as the thickness of the molecular layer increases, the effective volume of a molecule increases and hence the overall electron density of the layer decreases. All of these results of the LA molecule are similar to an unsaturated lipid molecule such as DOPC. ,− However, the collapse behavior is not similar to that of DOPC. ,,, In the collapsed region, even though the area/molecule is low, there is no indication of formation of multilayer as it is clear from this reflectivity study. In this region, the molecule probably dissolves into the water subphase as a function of increased pressure.…”

Interactions of bioactive molecules & nanomaterials with Langmuir monolayers as cell membrane models

“…DPPC was usually the phospholipid of choice in such investigations, since the saturation degree was found not to be a significant factor [134] and only slightly stronger interactions were found with saturated (DPPC) compared to unsaturated (DOPC, POPC) phospholipids [135]. AmB interacted with DPPC in mixed monolayers [136], indicating that not only sterols, but also phospholipids have to be taken into consideration in AmB's membrane activity. Due to the experimental procedure applied (methods of spreading, type of spreading solvent and presence of ions), the strongest deviations A C C E P T E D M A N U S C R I P T ACCEPTED MANUSCRIPT from ideality were observed at different proportions of AmB-to-DPPC, e.g.…”

“…addition to mixtures with PCs (DPPC, DOPC, POPC) [134][135][136][137] and with other phospholipids, e.g. DPPG [146] or dipalmitoyl phosphatidyl serine (DPPS) [147].…”