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Vd, Kallinikova; Pv, Matekin; Ta, Ogloblina; Mi, Leĭkina; Af, Kononenko; Nm, Sokolova; Ls, Pogodina

doi:10.1023/a:1016636419597

Cited by 21 publications

(4 citation statements)

References 34 publications

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“…Since 1975, when it was reported that infection by T. spiralis could inhibit sarcoma growth (Lubiniecki and Cypess, 1975), researchers have been studying its antineoplastic effects. Parasite crude proteins and their ESPs inhibit the proliferation of tumour cells in vitro and in vivo and may induce tumour cell apoptosis (Kallinikova et al 2001;Kim et al 2007;Darani et al 2009). Prior research has shown that T. spiralis or its active proteins may inhibit sarcoma, melanoma, colon cancer, lung cancer, stomach cancer, leukaemia, myeloma, breast cancer and other cancers (Deng et al 2013;Kang et al 2013;Vasilev et al 2015), and induce apoptosis in certain cancer cells (Wang et al 2009(Wang et al , 2013Vasilev et al 2015).…”

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confidence: 99%

Study on the mitochondrial apoptosis pathways of small cell lung cancer H446 cells induced by Trichinella spiralis muscle larvae ESPs

et al. 2017

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Trichinella spiralis (T.spiralis) muscle-larva (ML) excretory-secretory proteins (ESPs) contain antitumour-active substances. ESPs have been shown to inhibit tumour growth. To explore the effects of these proteins on small cell lung cancer cells and the possible mechanisms of their antineoplastic action, H446 SCLC cells were co-cultured with different concentrations of T. spiralis ML ESPs for 12, 24 and 48 h. Our results showed that T. spiralis ML ESPs significantly inhibited H446 cell proliferation, which was dose-and time-dependent. The results of flow cytometry testing indicate a clear apoptosis trend in H446 cells co-cultured with ESPs for 24 h. Reverse transcription polymerase chain reaction and Western blotting results showed increased expression of pro-apoptosis genes Bax, Cyt-C, Apaf-1, caspase-9 and caspase-3, compared with the negative control group, and decreased the expression of anti-apoptosis genes Bcl-2 and Livin. Our results suggest that T. spiralis ML ESPs can induce apoptosis in H446 cells through a mitochondrial pathway, which may be a mechanism of antineoplastic action in T. spiralis ML ESPs.

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confidence: 99%

Study on the mitochondrial apoptosis pathways of small cell lung cancer H446 cells induced by Trichinella spiralis muscle larvae ESPs

et al. 2017

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“…Parasites like Plasmodium or antigens from T. gondi and T. canis had also been tried to provoke immune response against tumor model of mice with success [6,30,31]. But reports on direct killing of cancer cells by protozoa are limited [5,8,26], particularly concerning those taking place following the path of apoptosis [26]. We have now succeeded in demonstrating that attenuated L. donovani causes apoptosis by p53 mediated pathway, in accordance with the hygiene theory [32,33].…”

Section: Discussionmentioning

confidence: 57%

Heat Killed Attenuated Leishmania Induces Apoptosis of HepG2 Cells Through ROS Mediated p53 Dependent Mitochondrial Pathway

Bose

Banerjee

Das

et al. 2016

Cell Physiol Biochem

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Background/Aims: Cytotoxic effect of attenuated Leishmania on liver cancer cells by inducing ROS generation. Methods: Spectrophotometric study to analyze cell death and levels of different active caspases. Flow cytometric study was done to analyze apoptosis induction and ROS generation and levels of different protein. Western blot analysis was performed to study the levels of protein. Confocal microscopy was done to ascertain the expression of different apoptotic markers. Results: We have now observed that attenuated Leishmania donovani UR6 also has potentiality towards growth inhibition of HepG2 cells and investigated the mechanism of action. The effect is associated with increased DNA fragmentation, rise in number of annexinV positive cells, and cell cycle arrest at G1 phase. The detection of unregulated levels of active PARP, cleaved caspases 3 and 9, cytosolic cytochrome C, Bax, and Bad, along with the observed downregulation of Bcl-2 and loss of mitochondrial membrane potential suggested the involvement of mitochondrial pathway. Enhanced ROS and p53 levels regulate the apoptosis of HepG2 cells. NAC was found to inhibit p53 production but PFT-α has no effect on ROS generation. In conclusion, Leishmania donovani UR6 efficiently induces apoptosis in HepG2 cells through ROS mediated p53 dependent mitochondrial pathway. Conclusion: It has been reported earlier that some parasites show prominent cytotoxic effect and prevent tumor growth. From our study we found that Leishmania donovani UR6 efficiently induced apoptosis in HepG2 cells through ROS mediated p53 dependent mitochondrial pathway. This study has rejuvenated the age old idea of bio-therapy.

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“…There are scientific evidences indicating that some parasitic and microbial infections interfere with tumor growth and have anticancer activities [1][2][3][4][5][6][7][8] , and it has been shown that some extracts of Trypanosoma cruzi have toxic effects on cancer cells in cell cultures [1,2]. Kim et al (2007) showed that Toxoplasma gondii inhibited Lewis lung carcinoma growth through induction of Th1 immune responses and inhibition of angiogenesis [3].…”

Section: Discussionmentioning

confidence: 99%

“…It has been shown that some parasitic infections induce antitumor activity against certain types of cancers [1][2][3][4][5][6]. We previously showed that mice immunized with Toxoplasma gondii tachyzoiter or Toxocara canis egg antigens and challenged with fibrosarcoma cells showed less solid tumor growth in comparison with mice that were not immunized but challenged with the same cells [7].…”

Section: Introductionmentioning

confidence: 99%

Toxoplasma gondii But Not Leishmania major or Trichomonas vaginalis Decreases Cell Proliferation and Increases Cell Death on Fibrosarcoma Cancer Cells in Culture Medium

Shirzad¹,

Khorami²,

Soozangar³

et al. 2012

WJV

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It has been shown that some parasites interfere with malignant tumor cells growth in vivo or in vitro. In this work anticancer activity of three live protozoan parasites; Toxoplasma gondii, Trichomonas vaginalis and Leishmania major on fibrosarcoma cells growth has been investigated in cell culture medium. In this experimental study, WEHI-164 fibrosarcoma cells treated with alive Toxoplasma gondii tachyzoite, Trichomonas vaginalis trophozoite or Leishmania major promastigote as case groups or left intact as control groups. Following 24 hours incubation the number of cells, lactate dehydrogenase (LDH) and apoptosis were determined in case and control groups. Toxoplasma gondii tachyzoite decreased cell proliferation and increased cell lyses' but it did not induce apoptosis. Trichomonas vaginalis or Leishmania major didn't show any effects on cell proliferation, cell lyses or apoptosis. Therefore Toxoplasma gondii may have anticancer activity and further works is recommended to understand the mechanisms of action.

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Cited by 21 publications

References 34 publications

Study on the mitochondrial apoptosis pathways of small cell lung cancer H446 cells induced by Trichinella spiralis muscle larvae ESPs

Study on the mitochondrial apoptosis pathways of small cell lung cancer H446 cells induced by Trichinella spiralis muscle larvae ESPs

Heat Killed Attenuated Leishmania Induces Apoptosis of HepG2 Cells Through ROS Mediated p53 Dependent Mitochondrial Pathway

<i>Toxoplasma gondii</i> But Not <i>Leishmania major</i> or <i>Trichomonas vaginalis</i> Decreases Cell Proliferation and Increases Cell Death on Fibrosarcoma Cancer Cells in Culture Medium

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Cited by 21 publications

References 34 publications

Study on the mitochondrial apoptosis pathways of small cell lung cancer H446 cells induced by Trichinella spiralis muscle larvae ESPs

Study on the mitochondrial apoptosis pathways of small cell lung cancer H446 cells induced by Trichinella spiralis muscle larvae ESPs

Heat Killed Attenuated Leishmania Induces Apoptosis of HepG2 Cells Through ROS Mediated p53 Dependent Mitochondrial Pathway

&lt;i&gt;Toxoplasma gondii&lt;/i&gt; But Not &lt;i&gt;Leishmania major&lt;/i&gt; or &lt;i&gt;Trichomonas vaginalis&lt;/i&gt; Decreases Cell Proliferation and Increases Cell Death on Fibrosarcoma Cancer Cells in Culture Medium

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<i>Toxoplasma gondii</i> But Not <i>Leishmania major</i> or <i>Trichomonas vaginalis</i> Decreases Cell Proliferation and Increases Cell Death on Fibrosarcoma Cancer Cells in Culture Medium