[42] Two simple programs for the analysis of data from enzyme-linked immunosorbent assays (ELISA) on a programmable desk-top calculator

Ritchie, David G.; Nickerson, John M.; Fuller, Gerald M.

doi:10.1016/0076-6879(83)92045-1

Cited by 19 publications

(1 citation statement)

References 5 publications

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“…The normalized values give a relative standard deviation in concentration units of 20% for the 0.5 gg/L standard and 15% for the 1.0 gg/L standard. Plotting percent inhibition versus concentration is similar to the logit plots commonly used in enzymology (20). "AOD, difference in optical density at 450 nm in absorbance units between sample and negative control determined with a differential spectrophotometer.…”

Section: Methodsmentioning

confidence: 99%

Enzyme-linked immunosorbent assay compared with gas chromatography/mass spectrometry for the determination of triazine herbicides in water

Thurman¹,

Meyer²,

Pomes³

et al. 1990

Anal. Chem.

307

147

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An enzyme-linked immunosorbent assay (ELISA) was compared to a gas chromatography/mass spectrometry (GC/MS) procedure for the analysis of triazine herbicides and their metabolites in surface water and groundwater. Apparent recoveries from natural water and spiked water by both methods were comparable at 0.2-2 micrograms/L. Solid-phase extraction (SPE) was examined also, and recoveries were determined for a suite of triazine herbicides. A significant correlation was obtained between the ELISA and GC/MS method for natural water samples that were extracted by SPE. Because ELISA was developed with an atrazine-like compound as the hapten with conjugation at the 2-position, it was selective for triazines that contained both ethyl and isopropyl side chains. Concentrations for 50% inhibition (IC50) were as follows: atrazine, 0.4 microgram/L; ametryne, 0.45 microgram/L; prometryn and propazine, 0.5 microgram/L; prometon, 0.7 microgram/L; simazine and terbutryn, 2.5 micrograms/L; hydroxyatrazine, 28 micrograms/L; deethylatrazine and deisopropylatrazine, 30 micrograms/L; cyanazine, 40 micrograms/L; didealkylatrazine had no response. The combination of screening analysis by ELISA, which requires no sample preparation and works on 160 microL of sample, and confirmation by GC/MS was designed for rapid, inexpensive analysis of triazine herbicides in water.

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Section: Methodsmentioning

confidence: 99%

Enzyme-linked immunosorbent assay compared with gas chromatography/mass spectrometry for the determination of triazine herbicides in water

Thurman¹,

Meyer²,

Pomes³

et al. 1990

Anal. Chem.

307

147

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Detection and determination of factor C ? a regulatory protein ?in Streptomyces strains by antiserum and monoclonal antibody

Szeszák¹,

Vitális²,

Tóth

et al. 1990

Arch. Microbiol.

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Rabbit antisera and monoclonal antibodies were raised against factor C, a regulatory protein of Streptomyces griseus. ELISA and immunoblotting techniques suitable to determine and characterize factor C antigen was detected in all the 23 Streptomyces strains and variants examined thus far and in one Bacillus subtilis too. Depending on the strain analysed it has a molecular mass of 34,000 or 70,000 in mycelial homogenates. Most of factor C was found excreted into the cultivation medium. The quantity of factor C antigen in different Streptomyces strains showed great variation. Amy+ strains were usually good producers of factor C while Amy- were not. This was consistent with our assumption that factor C was an inducer of reproductive phase in Streptomyces.

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Immunochemical studies onRhodotorula gracilis D-amino acid oxidase

Pollegioni

Simonetta

1991

Experientia

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Polyclonal antibodies were prepared from rabbit sera after immunization with holo- and apo-D-amino acid oxidase purified from R. gracilis. Both anti-holo- and anti-apoenzyme IgG fractions (as well as affinity-purified IgG) were highly specific: in blot-transfer analyses after SDS-PAGE only a 39 kDa band, corresponding to enzyme monomer, was recognized even in the partially purified yeast extract. No cross-reaction was detected with pig kidney D-amino acid oxidase. As a difference from the mammalian enzyme, yeast D-amino acid oxidase anti-holo- and anti-apoenzyme IgGs had different properties in inactivation and precipitation experiments, indicating the existence of different antigenicity sites related to the FAD-binding domain in the enzyme.

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[42] Two simple programs for the analysis of data from enzyme-linked immunosorbent assays (ELISA) on a programmable desk-top calculator

Cited by 19 publications

References 5 publications

Enzyme-linked immunosorbent assay compared with gas chromatography/mass spectrometry for the determination of triazine herbicides in water

Enzyme-linked immunosorbent assay compared with gas chromatography/mass spectrometry for the determination of triazine herbicides in water

Detection and determination of factor C ? a regulatory protein ?in Streptomyces strains by antiserum and monoclonal antibody

Immunochemical studies onRhodotorula gracilis D-amino acid oxidase

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