[42] Determination of aldehydic lipid peroxidation products: Malonaldehyde and 4-hydroxynonenal

Heparin protection against Fe2+‐and Cu2+‐mediated oxidation of liposomes

“…The samples were incubated at 40°C for 180 rain. In the case of Cu 2+-induced oxidation, where a lower concentration of liposomes was used, higher sensitivity was required and TBARS concentration was determined fluorimetrically at 553 nm with excitation at 515 nm [12]. As we recently reported elsewhere [13], a direct HPLC determination of TBA-MDA complex and other TBARS produced after liposome peroxidation showed that the standard TBA test (using trichloroacetic acid supernatant) measured exclusively MDA in peroxidized liposomes under the used experimental conditions.…”

“…The end products of lipid peroxidation were assayed using the thiobarbituric acid (TBA) method after trichloroacetic acid precipitation [12]. In the case of Fe2+-induced oxidation, the incubation mixtures contained 35 tll of liposome suspension (2.25 mM as organic phosphate), 115/al of PBS containing different HE amounts and either 150 /al of water or 150/al of Fenton reagent (2 mM H202, l mM FeSOz).…”

Influence of Microcystin-LR on the activity of membrane enzymes in rat intestinal mucosa

“…Measurement of lipid peroxidation levels.-Lipid peroxidation status was estimated by measuring the levels of malondialdehyde (MDA) in homogenized intestinal mucosa and serum samples, according to the procedure described for thiobarbituric acid reactive susbtances (TBARS) (7). Briefly, 0.5 mL of sample, previously treated with 25 µL of 1% (w/v) butylated hydroxytoluene in glacial acetic acid, were mixed with 0.2 mL of 8% (w/v) sodium lauryl sulphate, 1 mL of 20 % (v/v) acetic acid and 1 mL of 0.8% (w/v) 2-thiobarbituric acid.…”

Effect of methylprednisolone, tirilazad mesylate and vitamin E on lipid peroxidation after experimental spinal cord injury

“…Tissue malondialdehyde levels were directly determined by the ionpaired reversed phase HPLC method with UV detection as previously described by Esterbauer and Cheeseman. 32 Following the removal of proteins from tissue homogenates by the acetonitrile treatment and centrifugation, clear supernatant is injected into the HPLC system. Malondialdehydbis-(dimethylacetal) (Merck) was used as external standards and concentrations were expressed as nmol MDA/g wet weight of the tissues.…”