Effect of methylprednisolone, tirilazad mesylate and vitamin E on lipid peroxidation after experimental spinal cord injury

Abstract

E ect of methylprednisolone (MP), tirilazad mesylate (TM) and vitamin E on lipid peroxidation (LP) was evaluated in an experimental model of spinal cord compression injury in anesthetized rats. Forty rats, divided randomly into four groups, were injured by compressing on the spinal cord at Th 3 for 1 min. Bolus injections of saline solution, MP (30 mg/kg bolus and 5.4 mg/kg/h), TM (10 mg/kg four times per day), or vitamin E (30 mg/ kg four times per day) were begun 1 h after the spinal cord injury (SCI). Twenty-four hours after treatment, the rats were killed, and malondialdehyde (MDA), a LP product, was measured in the spinal cord tissues. Rats treated with MP, TM and vitamin E had signi®cantly decreased MDA levels (P50.01) than rats in the control group. The lowest MDA levels were found in the TM group. These results suggest that MP, TM and vitamin E may have a protective e ect against SCI in rats by its antioxidant e ect.

Comparison of the effects of octreotide and melatonin in preventing nerve injury in rats with experimental spinal cord injury

“…17 The spinal cord and brain are particularly vulnerable to free radical oxidation following hypoxic or traumatic insult because of their high lipid content and poor iron-binding capacity. Anti-inflammatory 10 and/or immunosuppressive drugs, 18 phospholipase inhibitors, cyclo-oxygenase/lipoxygenase/mixed lipoxygenase-cyclo-oxygenase inhibitors, thromboxane synthetase, thromboxane, and leucotriene receptor antagonists, 13,14 ROS scavengers/antioxidants, 17,19 biological enzymes, 14,17 vitamins, 20,21 selenium cations, 22 ubiquinole, 21 glucose depletion, 22 spinal cord blood flow restoration, 23 hyperbaric oxygen therapy, 24 hypothermia, 25 epidural cord cooling, 26 methylprednisolone, 19,20,27,28 dextromethorphan, 28 etomidate, 29 hyperatum perforatum extract, 30 resveratrol, 19 protein synthesis inhibitor, 31 magnesium, 32 lipoic acid, 33 adenosine, 35 opiate antagonists, 14 and calcium channel antagonists, 36 have been trialed as treatments for SCI, and found promising. Despite the high concentrations of monoamines on SCI, the use of a-and b-catecholamine antagonists has not been useful in clinical practice.…”

Antioxidative therapy in contusion spinal cord injury

“…These events are called secondary injury, and include spinal cord ischemia and free radical, among other events. 1,2 It can be inferred that there is an association between free radicals and spinal cord injury, from observations that these products present increased concentrations following spinal injury, that their production can be reduced using steroid antioxidants 3 and that there are lower concentrations of endogenous antioxidants following the injury. 4 Among the various types of free radicals, it seems that those of interest in relation to spinal cord injury look are oxygen derived (O 2 , H 2 O 2 or OH).…”

“…5,6 It has already been proven that vitamin E is important for the structural and functional maintenance of nerve tissue, to avoid free radical action on cell membranes. 1,2 However, it is also supposed that vitamin E administration to individuals with acute spinal cord injury would have no effect, as its absorption rate is low and chronic use before the injury would be required, for protective effects to be achieved. 6 Vitamin C returns vitamin E to its antioxidant status through reductive action, and may boost its effects.…”

Duration of lipid peroxidation after acute spinal cord injury in rats and the effect of methylprednisolone

“…Based on previously reported studies, 24,32,35,40,41 the animals treated with methylprednisolone received an initial 30-mg/kg bolus intraperitoneally, followed by an infusion of 5.4 mg/kg/hr. Control animals received equal volume injections of vehicle (normal saline).…”