4-(N-Methylhydrazino)-7-nitro-2,1,3-benzooxadiazole (MNBDH): A Novel Fluorogenic Peroxidase Substrate

Meyer, Jörg; Büldt, Andrea; Vogel, Martin; Kärst, Uwe

doi:10.1002/(sici)1521-3773(20000417)39:8<1453::aid-anie1453>3.0.co;2-7

Cited by 18 publications

(13 citation statements)

References 2 publications

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“…The optimization is therefore the first and necessary step in the development of sensors using new peroxidase substrates. MNBDH was first used as a substrate for horseradish peroxidase by Meyer et al [ 22 ]. This compound is nonfluorescent as a hydrazine derivative, but it can be oxidized by H 2 O 2 in the presence of HRP or DNAzyme to N -methyl-4-amino-7-nitrobezofurazan (MNBDA), as shown in Fig.…”

Section: Resultsmentioning

confidence: 99%

N-Methyl-4-hydrazino-7-nitrobenzofurazan: a fluorogenic substrate for peroxidase-like DNAzyme, and its potential application

Kosman

Głuszyńska

et al. 2014

Anal Bioanal Chem

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Characterization and optimization studies of N-methyl-4-hydrazino-7-nitrobenzofurazan (MNBDH) as a new fluorogenic substrate in the peroxidation reaction catalyzed by DNAzyme are reported. The effects of pH, H2O2 concentration, metal-cation type, and the concentration and type of surfactant on the fluorescence intensity were investigated. The optimized reaction was subsequently used for the development of an assay for DNA detection based on a molecular-beacon probe. The use of a fluorogenic substrate enabled the detection of a single-stranded DNA target with a 1 nmol L−1 detection limit.Graphical Abstractᅟ

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Section: Resultsmentioning

confidence: 99%

N-Methyl-4-hydrazino-7-nitrobenzofurazan: a fluorogenic substrate for peroxidase-like DNAzyme, and its potential application

Kosman

Głuszyńska

et al. 2014

Anal Bioanal Chem

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“…The reason is its slow conversion by horseradish peroxidase (POD), which allows the use of MNBDH as a substrate only in those cases when POD is present in excess concentrations. Therefore, the determination of hydrogen peroxide, glucose or glucose oxidase leads to favorable analytical figures of merit [27], while the attempt to detect POD itself does not. More recently, it was found that, actually, MNBDH may rapidly be converted by microperoxidases [24].…”

Section: Resultsmentioning

confidence: 99%

Analysis of microperoxidases using liquid chromatography, post-column substrate conversion and fluorescence detection

Haselberg¹,

Hempen²,

Leeuwen³

et al. 2006

Journal of Chromatography B

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“…The dimer is highly fluorescent and has already been used for the detection of several different hydroperoxides. [12][13][14] Especially for TATP, where the LOD of the pHPAA method is by one decade lower than that of the ABTS method, this variation is attractive, although a more complex and time-consuming experimental setup has to be used.…”

Section: Resultsmentioning

confidence: 99%

A field test for the detection of peroxide-based explosives

Schulte-Ladbeck

Kolla

Kärst

2002

Analyst

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A rapid and simple field test for the detection of triacetone-triperoxide (TATP) and hexamethylenetriperoxidediamine (HMTD), two explosives which find significant illegal use, has been developed. Unknown samples are first treated with a catalase solution to remove hydrogen peroxide traces, in order to provide selectivity towards peroxide-based bleaching agents which are contained in commercial laundry detergents. Subsequently, the peroxide-based explosives are decomposed via UV irradiation, thus yielding hydrogen peroxide, which is determined by the horseradish peroxidase (POD) catalysed formation of the green radical cation of 2,2'-azino-bis(3-ethylbenzothiazoline)-6-sulfonate (ABTS). The limits of detection for this method are 8 x 10(-6) mol dm(-3) for TATP and 8 x 10(-7) mol dm(-3) for HMTD, respectively. As an option, p-hydroxyphenylacetic acid (pHPAA) may be used as peroxidase substrate, resulting in lower limits of detection (8 x 10(-7) mol dm(-3) for TATP and HMTD). The complete method uses a mobile setup to be applied under field conditions.

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4-(N-Methylhydrazino)-7-nitro-2,1,3-benzooxadiazole (MNBDH): A Novel Fluorogenic Peroxidase Substrate

Cited by 18 publications

References 2 publications

N-Methyl-4-hydrazino-7-nitrobenzofurazan: a fluorogenic substrate for peroxidase-like DNAzyme, and its potential application

N-Methyl-4-hydrazino-7-nitrobenzofurazan: a fluorogenic substrate for peroxidase-like DNAzyme, and its potential application

Analysis of microperoxidases using liquid chromatography, post-column substrate conversion and fluorescence detection

A field test for the detection of peroxide-based explosives

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