4-methoxycinnamyl p-coumarate isolated from Etlingera pavieana rhizomes inhibits inflammatory response via suppression of NF-κB, Akt and AP-1 signaling in LPS-stimulated RAW 264.7 macrophages

Mankhong, Sakulrat; Iawsipo, Panata; Srisook, Ekaruth; Srisook, Klaokwan

doi:10.1016/j.phymed.2018.09.193

Cited by 28 publications

(25 citation statements)

References 26 publications

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“…Mounting evidence suggests that transcription factors such as NF-κB and AP-1 are tightly involved in regulating inflammation through the activation of diverse inflammatory genes, including iNOS, COX-2, TNF-α, IL-1β, and IL-6 [3]. NF-κB activation in inflammatory cells depends on the phosphorylation-induced protein degradation of the inhibitor of NF-κB protein (IκBs), which sequesters inactive NF-κB dimers in the cytosol of resting cells [5].…”

Section: Discussionmentioning

confidence: 99%

“…Various innate immune cells such as macrophages, fibroblasts, mast cells, and neutrophils are activated in response to infection. Among these responses, the activation of macrophages plays a pivotal role in the progression of multiple inflammatory diseases via the release of large amounts of nitric oxide (NO), prostaglandin (PG), and pro-inflammatory cytokines, such as tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), and -6 (IL-6), and reactive oxygen species (ROS) [2,3]. Therefore, inhibiting these pro-inflammatory mediators and cytokines in activated macrophages should facilitate the treatment of inflammatory diseases.…”

Section: Introductionmentioning

confidence: 99%

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Cerevisterol Alleviates Inflammation via Suppression of MAPK/NF-κB/AP-1 and Activation of the Nrf2/HO-1 Signaling Cascade

et al. 2020

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As part of our continuous effort to find potential anti-inflammatory agents from endophytic fungi, a Fusarium solani strain, isolated from the plant Aponogeton undulatus Roxb., was investigated. Cerevisterol (CRVS) was identified from endophytic fungi, a Fusarium solani strain, and moreover exhibited anti-inflammatory activity. However, the underlying mode of action remains poorly understood. The aim of this study is to reveal the potential mechanisms of CRVS against inflammation on a molecular level in LPS-activated RAW 264.7 peritoneal macrophage cells. CRVS was isolated from F. solani and characterized based on spectral data analysis. The MTT assay was performed to measure cell viability in CRVS-treated macrophages. Anti-inflammatory activity was assessed by measurement of nitric oxide (NO) and prostaglandin E2 (PGE2) levels, as well as the production of various cytokines, such as tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), and -6 (IL-6) in LPS-stimulated macrophages. RT-PCR and immunoblotting analyses were done to examine the expression of various inflammatory response genes. A reporter gene assay was conducted to measure the level of nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) and activator protein-1 (AP-1) transactivation. CRVS suppresses the LPS-induced production of NO and PGE2, which is a plausible mechanism for this effect is by reducing the expression of iNOS and COX-2. CRVS also decreases the expression of pro-inflammatory cytokines, such as TNF-α, IL-6, and IL-1β. CRVS halted the nuclear translocation of NF-κB by blocking the phosphorylation of inhibitory protein κBα (IκBα) and suppressing NF-κB transactivation. The mitogen-activated protein kinases (MAPK) signaling pathways are also suppressed. CRVS treatment also inhibited the transactivation of AP-1 and the phosphorylation of c-Fos. Furthermore, CRVS could induce the nuclear translocation of nuclear factor erythroid 2-related factor 2 (Nrf2) by down-regulating Kelch-like ECH-associated protein 1 (Keap-1) and up-regulating hemeoxygenases-1 (HO-1) expression. The results suggest that CRVS acts as a natural agent for treating inflammatory diseases by targeting an MAPK, NF-κB, AP-1, and Nrf2-mediated HO-1 signaling cascade.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Cerevisterol Alleviates Inflammation via Suppression of MAPK/NF-κB/AP-1 and Activation of the Nrf2/HO-1 Signaling Cascade

et al. 2020

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“…It consists of the members of Jun, Fos, or ATF families. The activation of AP-1 involved the heterogeneous dimerization of Jun and Fos, and the recognition of the transcription site [33]. Treatment with EP2 induced the expression of c-Jun and c-Fos after 1 h. The activation of transcription factors promotes the synthesis and release of inflammatory factors.…”

Section: Discussionmentioning

confidence: 99%

Immunomodulatory Effects of a Low-Molecular Weight Polysaccharide from Enteromorpha prolifera on RAW 264.7 Macrophages and Cyclophosphamide- Induced Immunosuppression Mouse Models

Liu

Jin

et al. 2020

Marine Drugs

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The water-soluble polysaccharide EP2, from Enteromorpha prolifera, belongs to the group of polysaccharides known as glucuronoxylorhamnan, which mainly contains glucuronic acid (GlcA), xylose (Xyl), and rhamnose (Rha). The aim of this study was to detect the immunomodulatory effects of EP2 on RAW 264.7 macrophages and cyclophosphamide (CYP)-induced immunosuppression mouse models. The cells were treated with EP2 for different time periods (0, 0.5, 1, 3, and 6 h). The results showed that EP2 promoted nitric oxide production and up-regulated the expression of pro-inflammatory cytokines, such as IL-1β, IL-6, and TNF-α, in a time-dependent manner. Furthermore, we found that EP2-activated iNOS, COX2, and NLRP3 inflammasomes, and the TLR4/MAPK/NF-κB signaling pathway played an important role. Moreover, EP2 significantly increased the body weight, spleen index, thymus index, inflammatory cell counts, and the levels of IL-1β, IL-6, and TNF-α in CYP-induced immunosuppression mouse models. These results indicate that EP2 might be a potential immunomodulatory drug and provide the scientific basis for the comprehensive utilization and evaluation of E. prolifera in future applications.

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“…It is reported that E. pavieana possess various pharmacological activities such as antioxidant [10,11]; antimicrobial [7,12]; anticancer against breast, cervix, liver, colon, and small cell lung cancer [7,13]; and anti-inflammatory effects [8,[14][15][16]. The detailed information is shown below.…”

Section: Pharmacological Activities Of E Pavieanamentioning

confidence: 99%

“…Mankhong et al reported that 4-methoxycinnamyl p-coumarate, as the most potent compound extracted from E. pavieana rhizomes, exhibited an inhibitory effect on the production of cyclooxygenase-2 (COX-2)-catalyzed PGE 2 , NO, IL-1β, and TNF-α on LPS-stimulated RAW 264.7 murine macrophages with IC 50 values of 14.1 ± 2.3, 32.7 ± 4.7, 3.0 ± 0.5, and 26.8 ± 5.7 μM, respectively. MCC compound inhibited gene expression of iNOS, COX-2, IL-1β, and TNF-α in a concentrationdependent manner through downregulating NF-κB, PI3K/Akt, and AP-1 signaling pathways [14,16]. Furthermore, MCC attenuated LPS-reduced COX-1 expression in LPS-stimulated RAW 264.7 macrophages.…”

Section: Anti-inflammatory Effectmentioning

confidence: 99%

Pharmacological Activities and Phytochemicals of Etlingera pavieana (Pierre ex Gagnep) R.M.Sm

Srisook¹,

Srisook²

2020

Medicinal Plants - Use in Prevention and Treatment of Diseases

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Etlingera pavieana (Pierre ex Gagnep) R.M.Sm. (Zingiberaceae family) is commonly found in Southeast Asia. The rhizome of the plant is used as a spice and folk medicine in southeastern Thailand and Cambodia. The extracts, essential oil, and compounds from E. pavieana were found to possess a variety of pharmacological activities like anti-inflammatory, antioxidant, antiatherogenic, and antimicrobial effects. Furthermore, phytochemical studies have reported the presence of various chemical constituents, the main being phenylpropanoids such as trans-4-methoxycinnamaldehyde (MCD) and 4-methoxycinnamyl p-coumarate (MCC). Therefore, E. pavieana seems to be a potential source of natural products for treatment of various diseases and promotion of good health.

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4-methoxycinnamyl p-coumarate isolated from Etlingera pavieana rhizomes inhibits inflammatory response via suppression of NF-κB, Akt and AP-1 signaling in LPS-stimulated RAW 264.7 macrophages

Cited by 28 publications

References 26 publications

Cerevisterol Alleviates Inflammation via Suppression of MAPK/NF-κB/AP-1 and Activation of the Nrf2/HO-1 Signaling Cascade

Cerevisterol Alleviates Inflammation via Suppression of MAPK/NF-κB/AP-1 and Activation of the Nrf2/HO-1 Signaling Cascade

Immunomodulatory Effects of a Low-Molecular Weight Polysaccharide from Enteromorpha prolifera on RAW 264.7 Macrophages and Cyclophosphamide- Induced Immunosuppression Mouse Models

Pharmacological Activities and Phytochemicals of Etlingera pavieana (Pierre ex Gagnep) R.M.Sm

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