4-chloro eugenol interacts synergistically with artesunate against drug resistant P. falciparum inducing oxidative stress

Mina, Pooja Rani; Kumar, Sunil; Agarwal, Krishna; Kumar, Ravi; Pal, Anirban; Tandon, Sudeep; Yadav, Sanjeev Kumar Singh; Yadav, Sanjay; Darokar, Mahendra P.

doi:10.1016/j.biopha.2021.111311

Cited by 10 publications

(5 citation statements)

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“…The hemolytic assay was performed according to ref. 33. Briefly, 1000 mL of 10% (v/v) red blood cells (RBC) suspension containing 50 mL of 18-b glycyrrhetinic acid (18-b GTA) and derivatives at 100 mM concentration, respectively, were incubated under agitation at room temperature.…”

Section: Hemolysis Assaymentioning

confidence: 99%

Design and synthesis of novel glycyrrhetinic acid-triazole derivatives that exert anti-plasmodial activity inducing mitochondrial-dependent apoptosis in Plasmodium falciparum

Kapkoti

Kumar

et al. 2023

New J. Chem.

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Section: Hemolysis Assaymentioning

confidence: 99%

Design and synthesis of novel glycyrrhetinic acid-triazole derivatives that exert anti-plasmodial activity inducing mitochondrial-dependent apoptosis in Plasmodium falciparum

Kapkoti

Kumar

et al. 2023

New J. Chem.

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“…Although malaria is life-threatening, early diagnosis and appropriate treatment lead to it having an excellent prognosis [ 25 ]. Global data show that around 229 million people are affected by malaria, while about 409,000 succumb to it annually; hence, efforts are needed to prevent and control it [ 26 ]. Children under the age of 5 years and pregnant women are at a higher risk of being attacked by malaria and have a greater likelihood of developing complications [ 27 ].…”

Section: Malaria and Its Parasite Life Cyclementioning

confidence: 99%

“…In vivo antiplasmodial activity using Swiss albino mice infected with P. yoelli nigeriensis showed that a combination of ART and 4CE had chemo-suppression of 91.4% compared with 55% and 47% for ART (9.5 mg/kg) and 4CE (88 mg/kg), respectively ( Table 1 ). The results depicted below showed dose-dependency ( Table 1 ) [ 26 ].…”

Section: Malaria and Its Parasite Life Cyclementioning

confidence: 99%

“…The combination (2×ED50 of ART + 4CE) reduced blood–brain integrity loss (4.73 µg/g of Evan blue) compared with the individual drugs which had 20.99 µg/g and 17.98 µg/g of Evan’s blue visible for 4CE (166 mg/kg) and ART (20 mg/kg), respectively. The results showed that 4CE can synergistically interact with ART to induce oxidative stress against drug-resistant P. falciparum [ 26 ].…”

Section: Malaria and Its Parasite Life Cyclementioning

confidence: 99%

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Recent Advances in the Therapeutic Efficacy of Artesunate

2022

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Artesunate, a semisynthetic artemisinin derivative, is well-known and used as the first-line drug for treating malaria. Apart from treating malaria, artesunate has also been found to have biological activity against a variety of cancers and viruses. It also exhibits antidiabetic, anti-inflammatory, anti-atherosclerosis, immunosuppressive activities, etc. During its administration, artesunate can be loaded in liposomes, alone or in combination with other therapeutic agents. Administration routes include intragastrical, intravenous, oral, and parenteral. The biological activity of artesunate is based on its ability to regulate some biological pathways. This manuscript reports a critical review of the recent advances in the therapeutic efficacy of artesunate.

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“…Plants have been reported in combination therapy to possess either anti-plasmodial activity or synergy with antimalarial drugs, leading to attenuation of cerebral malaria pathogenesis. Recently, previous studies have demonstrated that a combination of 4-chloro eugenol, a semisynthetic of phytomolecules eugenol, and artesunate showed higher parasitemia suppression and prolonged mean survival time in P. berghei infected mice [ 17 ]. Oleanane triterpenoids given as adjunctive therapy in combination with artesunate significantly improved survival in ECM through reactive oxygen species scavenging and blood–brain barrier integrity improvement over artesunate alone [ 18 ].…”

Section: Introductionmentioning

confidence: 99%

Efficacy of artesunate combined with Atractylodes lancea or Prabchompoothaweep remedy extracts as adjunctive therapy for the treatment of cerebral malaria

Plirat,

Chaniad,

Phuwajaroanpong

et al. 2023

BMC Complement Med Ther

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Background Cerebral malaria is one of the most serious complications of Plasmodium infection and causes behavioral changes. However, current antimalarial drugs have shown poor outcomes. Therefore, new antimalarials with neuroprotective effects are urgently needed. This study aimed to evaluate the effects of selected extracts as monotherapy or adjunctive therapy with artesunate on antimalarial, anti-inflammatory, antioxidant, and neuroprotective properties in experimental cerebral malaria (ECM). Methods ECM was induced in male C57BL/6 mice by infection with Plasmodium berghei ANKA (PbA). Ethanolic extracts of Atractylodes lancea (a dose of 400 mg/kg) and Prabchompoothaweep remedy (a dose of 600 mg/kg) were evaluated as monotherapy and adjunctive therapy combined with artesunate at the onset of signs of cerebral malaria and continued for 7 consecutive days. Parasitemia, clinical scores, and body weight were recorded throughout the study. At day 13 post-infection, mouse brains were dissected and processed for the study of the inflammatory response, oxidative stress, blood–brain barrier (BBB) integrity, histopathological changes, and neurocognitive impairments. Results Ethanolic extracts of A. lancea and Prabchompoothaweep remedy alone improved cerebral malaria outcome in ECM, whereas artesunate combined with extracts of A. lancea or Prabchompoothaweep remedy significantly improved the outcome of artesunate and crude extracts alone. Using real-time PCR, PbA-infected mice that had received the combination treatment showed significantly reduced gene expression of inflammatory cytokines (TNF-α, IL-1β, IL-6, and IL-10), chemokines (CXCL4 and CXCL10), and adhesion molecules (ICAM-1, VCAM1, and CD36). The PbA-infected mice that received the combination treatment showed a significantly decreased malondialdehyde level compared to the untreated group. Similarly, the Evans blue dye assay revealed significantly less dye extravasation in the brains of infected mice administered the combination treatment, indicating improved BBB integrity. Combination treatment improved survival and reduced pathology in the PbA-infected group. Additionally, combination treatment resulted in a significantly reduced level of cognitive impairment, which was analyzed using a novel object recognition test. Conclusions This study demonstrated that artesunate combined with A. lancea or Prabchompoothaweep remedy extracts as adjunctive therapy reduced mortality, neuroinflammation, oxidative stress, BBB integrity protection, and neurocognitive impairment in the ECM.

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4-chloro eugenol interacts synergistically with artesunate against drug resistant P. falciparum inducing oxidative stress

Cited by 10 publications

References 45 publications

Design and synthesis of novel glycyrrhetinic acid-triazole derivatives that exert anti-plasmodial activity inducing mitochondrial-dependent apoptosis in Plasmodium falciparum

Design and synthesis of novel glycyrrhetinic acid-triazole derivatives that exert anti-plasmodial activity inducing mitochondrial-dependent apoptosis in Plasmodium falciparum

Recent Advances in the Therapeutic Efficacy of Artesunate

Efficacy of artesunate combined with Atractylodes lancea or Prabchompoothaweep remedy extracts as adjunctive therapy for the treatment of cerebral malaria

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