In this paper, karyology of thirty one accessions of Safflower (Carthamus tinctorius L.) were investigated in terms of their chromosome numbers and karyomorphology. The chromosomal counts confirmed the results of previous reports, that the Carthamus has same basic chromosome numbers. According to our results discussed all accessions of chromosome numbers have been identified 2n = 24, x=12 and also all have the diploid number of chromosomes. We found predominance of chromosomes being metacentric and sub-metacentric in karyotypes. Five quantitative asymmetry indices were used to evaluate our karyological results in all species and elucidate the chromosomal alterations of Carthamus tinctorius accessions. All karyotyping analyses were described for the first time in this report via KAMERAM programme. We hope that these findings would be contributed for Carthamus genetic and breeding studies.