3D scaffold with effective multidrug sequential release against bacteria biofilm

García-Álvarez, Rafaela; Izquierdo‐Barba, Isabel; Vallet‐Regí, María

doi:10.1016/j.actbio.2016.11.028

Cited by 74 publications

(50 citation statements)

References 67 publications

(62 reference statements)

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“…However, our results suggest that in addition to the drug diffusion process throughout the mesoporous matrix, a new component is governing the drug release kinetics. Specifically, this new component refers to the MGHA matrix-Levo interactions, as it has been previously reported for other silica matrix [44,45]. Fig.…”

Section: In Vitro Levofloxacin Release Kineticsmentioning

confidence: 86%

Multifunctional pH sensitive 3D scaffolds for treatment and prevention of bone infection

et al. 2018

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Multifunctional 3D nanocomposite scaffolds with the ability for loading and sustained delivery of an antimicrobial agent, to eliminate and prevent bone infection and at the same time to contribute to bone regeneration process without cytotoxic effects on the surrounding tissue has been proposed. These 3D scaffolds exhibit a sustained levofloxacin delivery at physiological pH (pH 7.4), which increasing notably when pH decreases to characteristic values of bone infection process (pH 6.7 and pH 5.5). In vitro competitive assays between preosteoblastic and bacteria onto the 3D scaffold surface demonstrated an adequate osteoblast colonization in entire scaffold surface together with the ability to eliminate bacteria contamination.

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Section: In Vitro Levofloxacin Release Kineticsmentioning

confidence: 86%

Multifunctional pH sensitive 3D scaffolds for treatment and prevention of bone infection

et al. 2018

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“…In both cases, δ is minor than 1, showing that the behavior is different from a first order kinetic. The k values varies from 0.021 to 0.51 h −1 for MSN@LEVO and MSN-DAMO@LEVO, respectively, which could point to strong attractive interactions of the LEVO molecules with the Si-OH groups present in the matrix of pristine MSN, as it has been demonstrated elsewhere [34]. The effectiveness of the different released LEVO doses at several times from both MSNs against E. coli and S. aureus bacterial growth was performed.…”

Section: Levofloxacin Loading and Release Assaysmentioning

confidence: 99%

Amine-Functionalized Mesoporous Silica Nanoparticles: A New Nanoantibiotic for Bone Infection Treatment

Pedraza¹,

Díez²,

Izquierdo‐Barba³

et al. 2018

Biomedical Glasses

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This manuscript reports an effective new alternative for the management of bone infection by the development of an antibiotic nanocarrier able to penetrate bacterial biofilm, thus enhancing antimicrobial effectiveness. This nanosystem, also denoted as "nanoantibiotic", consists in mesoporous silica nanoparticles (MSNs) loaded with an antimicrobial agent (levofloxacin, LEVO) and externally functionalized with N-(2-aminoethyl)-3-aminopropyltrimethoxysilane (DAMO) as targeting agent. This amine functionalization provides MSNs of positive charges, which improves the affinity towards the negatively charged bacteria wall and biofilm. Physical and chemical properties of the nanoantibiotic were studied using different characterization techniques, including Xray diffraction (XRD), transmission electron microscopy (TEM), N 2 adsorption porosimetry, elemental chemical analysis, dynamic light scattering (DLS), zeta (ζ )-potential and solid-state nuclear magnetic resonance (NMR). "In vial" LEVO release profiles and the in vitro antimicrobial effectiveness of the different released doses were investigated. The efficacy of the nanoantibiotic against a S. aureus biofilm was also determined, showing the practically total destruction of the biofilm due to the high penetration ability of the developed nanosystem. These findings open up promising expectations in the field of bone infection treatment.

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“…However, advances toward creating TECs for in vivo applications have led to progress in designing biomimetic in vitro models for studying bone biology, disease progression, and drug screening in the bone microenvironment. 3D in vitro bone models have been proposed to aid in bridging the gap between 2D culture and animal models for diseases and medical conditions such as osteomyelitis [33], bone fracture healing [34, 35], and, as will be discussed in this review, tumor metastases. In designing such TECs, studies investigating properties of the constructs indicated that characteristics including rigidity [32, 36], pore size [37, 38], pore shape [39], and curvature [40] all affect cell behavior.…”

Section: D Bone Modelsmentioning

confidence: 99%

Engineering 3D Models of Tumors and Bone to Understand Tumor-Induced Bone Disease and Improve Treatments

Kwakwa

Vanderburgh

Guelcher

et al. 2017

Curr Osteoporos Rep

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Purpose of Review Bone is a structurally unique microenvironment that presents many challenges for the development of 3D models for studying bone physiology and diseases, including cancer. As researchers continue to investigate the interactions within the bone microenvironment, the development of 3D models of bone has become critical. Recent Findings 3D models have been developed that replicate some properties of bone, but have not fully reproduced the complex structural and cellular composition of the bone microenvironment. This review will discuss 3D models including polyurethane, silk, and collagen scaffolds that have been developed to study tumor-induced bone disease. In addition, we discuss 3D printing techniques used to better replicate the structure of bone. Summary 3D models that better replicate the bone microenvironment will help researchers better understand the dynamic interactions between tumors and the bone microenvironment, ultimately leading to better models for testing therapeutics and predicting patient outcomes.

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3D scaffold with effective multidrug sequential release against bacteria biofilm

Cited by 74 publications

References 67 publications

Multifunctional pH sensitive 3D scaffolds for treatment and prevention of bone infection

Multifunctional pH sensitive 3D scaffolds for treatment and prevention of bone infection

Amine-Functionalized Mesoporous Silica Nanoparticles: A New Nanoantibiotic for Bone Infection Treatment

Engineering 3D Models of Tumors and Bone to Understand Tumor-Induced Bone Disease and Improve Treatments

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