3D printing of layered brain-like structures using peptide modified gellan gum substrates

Lozano, Rodrigo; Stevens, Leo; Thompson, Brianna C.; Gilmore, Kerry J.; Gorkin, Robert; Stewart, Elise M.; Panhuis, Marc in het; Romero-Ortega, Mario I.; Wallace, Gordon G.

doi:10.1016/j.biomaterials.2015.07.022

Cited by 377 publications

(304 citation statements)

References 58 publications

(37 reference statements)

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“…These brain-like structures permit the generation of more accurate 3D in vitro microstructures with applications ranging from cell behavior studies to improving our understanding of brain injuries and neurodegenerative diseases. [120] Othon et al printed neuronal tissues using LAB with over 95% cell viability. Their results have been extended to primary cultured olfactory ensheathing cells (OECs), harvested from adult Sprague-Dawley rats.…”

Section: Bioprinted Neural Tissuementioning

confidence: 99%

“…[53] In these multicellular aggregates, the need for supporting gels or matrices is eliminated, the adverse effects 3D culture approach for generating a laminated cerebral cortex like structure from pluripotent stem cells. [57,58] Microfabrication Neuroprogenitor cells Microfluidic culture platform containing a relief pattern of soma and axonal compartments connected by microgrooves to direct, isolate, lesion, and biochemically analyze CNS axons [67,68] 3D bioprinting Primary human cortical neurons Discrete layers of primary neutrons in a RGD peptide-modified gellan gum [118][119][120] Intestine (Gut) Self-assembled Stem cells Identified intestinal stem cells and differentiated cells in vitro [59,60] Microfabrication Human epithelial cells Mimic contractility by using mechanochemical actuator [11,19,27,72] Liver Self-assembled Human stem cells 3D culture of self-renewing human liver tissue [61,62] Microfabrication Hepatocytes and fibroblasts Microengineered hepatic microtissues containing hepatocytes and fibroblasts [73][74][75][76][77] 3D bioprinting HepG2 and HUVEC Multilayered organ tissue model [96,[155][156][157] Vessel Microfabrication Rat brain endothelial cells 3D culture in microfluidic device [63][64][65][66] 3D bioprinting HUVECs and HUVSMCs Scaffold-less vessel formation using spheroid fusion [84][85][86][87][88][89][90][91]…”

Section: Engineering Technologiesmentioning

confidence: 99%

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3D Miniaturization of Human Organs for Drug Discovery

Park

Wetzel

Dréau

et al. 2017

Adv Healthcare Materials

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“Engineered human organs” hold promises for predicting the effectiveness and accuracy of drug responses while reducing cost, time, and failure rates in clinical trials. Multiorgan human models utilize many aspects of currently available technologies including self‐organized spherical 3D human organoids, microfabricated 3D human organ chips, and 3D bioprinted human organ constructs to mimic key structural and functional properties of human organs. They enable precise control of multicellular activities, extracellular matrix (ECM) compositions, spatial distributions of cells, architectural organizations of ECM, and environmental cues. Thus, engineered human organs can provide the microstructures and biological functions of target organs and advantageously substitute multiscaled drug‐testing platforms including the current in vitro molecular assays, cell platforms, and in vivo models. This review provides an overview of advanced innovative designs based on the three main technologies used for organ construction leading to single and multiorgan systems useable for drug development. Current technological challenges and future perspectives are also discussed.

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Section: Bioprinted Neural Tissuementioning

confidence: 99%

Section: Engineering Technologiesmentioning

confidence: 99%

3D Miniaturization of Human Organs for Drug Discovery

Park

Wetzel

Dréau

et al. 2017

Adv Healthcare Materials

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“…In most cases, GG products need to be sterilised prior to use in cell culture and TE. Various techniques are in use for this purpose including autoclaving [34][35][36] , ethylene oxide treatment 37 , microfiltration 38 , and antibiotic supplements 39 . Although no studies directly compare these methods with respect to their impacts on GG, equivalent investigations using other polysaccharides [40][41][42][43] and ECM materials 40,44,45 provide a good indication of the likely effects on GG.…”

Section: Purification and Sterilisationmentioning

confidence: 99%

Tissue engineering with gellan gum

et al. 2016

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Engineering complex tissues for research and clinical applications relies on high-performance biomaterials that are amenable to biofabrication, maintain mechanical integrity, support specific cell behaviours, and, ultimately, biodegrade. In most cases, complex tissues will need to be fabricated from not one, but many biomaterials, which collectively fulfill these demanding requirements. Gellan gum is an anionic polysaccharide with potential to fill several key roles in engineered tissues, particularly after modification and blending. This review focuses on the present state of research into gellan gum, from its origins, purification and modification, through processing and biofabrication options, to its performance as a cell scaffold for both soft tissue and load bearing applications. Overall, we find gellan gum to be a highly versatile backbone material for tissue engineering research, upon which a broad array of form and functionality can be built. Disciplines Engineering | Physical Sciences and Mathematics Publication DetailsStevens, L. R., Gilmore, K. J., Wallace, G. Engineering complex tissues for research and clinical applications relies on high-performance biomaterials that are amenable to biofabrication, maintain mechanical integrity, support specific cell behaviours, and, ultimately, biodegrade. In most cases, complex tissues will need to be fabricated from not one, but many biomaterials, which collectively fulfill these demanding requirements. Gellan gum is an anionic polysaccharide with potential to fill several key roles in engineered tissues, particularly after modification and blending. This review focuses on the present state of research into gellan gum, from its origins, purification and modification, through processing and biofabrication options, to its performance as a cell scaffold for both soft tissue and load bearing applications. Overall, we find gellan gum to be a highly versatile backbone material for tissue engineering research, upon which a broad array of form and functionality can be built.

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“…One example is presented by our group and involves the development of a novel bioprintingbased method to recapitulate the layered structure of the brain and thereby fabricate 3D brain-like structures. [72] This study was based on the previous work by Tang-Schomer et al, who developed compartmentalized 3D brain-like cortical tissue with silk fibroin-based biomaterials. [17] Our group developed an Arg-Gly-Asp (RGD) peptide-modified gellan gum-based bioink (RGD-GG), which was assessed in terms of neural cytocompatibility, network formation, and printability.…”

Section: Bioprinting Technologiesmentioning

confidence: 99%

“…However, it is important to realize that different applications may require a different approach, which could be [72] with permission from Elsevier.…”

Section: Bioprinting: a Complementary Approachmentioning

confidence: 99%

Three-dimensional neuronal cell culture: in pursuit of novel treatments for neurodegenerative disease

et al. 2017

Self Cite

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To gain a better understanding of the underlying mechanisms of neurological disease, relevant tissue models are imperative. Over the years, this realization has fuelled the development of novel tools and platforms, which aim at capturing in vivo complexity. One example is the field of biofabrication, which focuses on fabrication of three-dimensional (3D) biologically functional products in a controlled and automated manner. Herein, we provide a general overview of classical 3D cell culture platforms, particularly in the context of neurodegenerative disease. Subsequently, the focus is put on bioprinting-based biofabrication, its potential to advance 3D neuronal cell culture and, to conclude, the relevant translational bottlenecks, which will need to be considered as the field evolves.

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3D printing of layered brain-like structures using peptide modified gellan gum substrates

Cited by 377 publications

References 58 publications

3D Miniaturization of Human Organs for Drug Discovery

3D Miniaturization of Human Organs for Drug Discovery

Tissue engineering with gellan gum

Three-dimensional neuronal cell culture: in pursuit of novel treatments for neurodegenerative disease

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