3D Printing of Cytocompatible Graphene/Alginate Scaffolds for Mimetic Tissue Constructs

Li, Jianfeng; Liu, Xiao; Crook, Jeremy Micah; Wallace, Gordon G.

doi:10.3389/fbioe.2020.00824

Cited by 44 publications

(31 citation statements)

References 47 publications

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“…Kill bacteria [ 81 ] coated with graphene oxide (GO) Alg 3D Bio-plotting Provide electrical conductivity and cell affinity sites. [ 82 ] miRNA-148 b-transfected PCL/PLGA/HAp 3D Bio-plotting Improved bone regeneration considerably. [ 84 ] electrical stimuli gelatin-graphene conduits FDM Have a profound effect on the differentiation of MSCs to SC-like phenotypes and their paracrine activity.…”

Section: Scaffold Modification After Printingmentioning

confidence: 99%

Applications of 3D printed bone tissue engineering scaffolds in the stem cell field

Wang

Guo

2021

Regenerative Therapy

120

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Due to traffic accidents, injuries, burns, congenital malformations and other reasons, a large number of patients with tissue or organ defects need urgent treatment every year. The shortage of donors, graft rejection and other problems cause a deficient supply for organ and tissue replacement, repair and regeneration of patients, so regenerative medicine came into being. Stem cell therapy plays an important role in the field of regenerative medicine, but it is difficult to fill large tissue defects by injection alone. The scientists combine three-dimensional (3D) printed bone tissue engineering scaffolds with stem cells to achieve the desired effect. These scaffolds can mimic the extracellular matrix (ECM), bone and cartilage, and eventually form functional tissues or organs by providing structural support and promoting attachment, proliferation and differentiation. This paper mainly discussed the applications of 3D printed bone tissue engineering scaffolds in stem cell regenerative medicine. The application examples of different 3D printing technologies and different raw materials are introduced and compared. Then we discuss the superiority of 3D printing technology over traditional methods, put forward some problems and limitations, and look forward to the future.

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Section: Scaffold Modification After Printingmentioning

confidence: 99%

Applications of 3D printed bone tissue engineering scaffolds in the stem cell field

Wang

Guo

2021

Regenerative Therapy

120

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“…The GO was then reduced using ascorbic acid to obtain Alg/rGO ECHs with electrical resistance of 1.5 kΩ/sq and compressive modulus of 195 kPa. Moreover, the fabricated composite ECHs showed excellent human adipose stem cell viability and mineral deposition (as shown in Figure 6 e–g), which has potential in tissue engineering as support for osteogenic induction [ 52 ].…”

Section: State-of-the-art 3d Printed Echs: Fabrication Propertiesmentioning

confidence: 99%

3D Printable Electrically Conductive Hydrogel Scaffolds for Biomedical Applications: A Review

et al. 2021

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Electrically conductive hydrogels (ECHs), an emerging class of biomaterials, have garnered tremendous attention due to their potential for a wide variety of biomedical applications, from tissue-engineered scaffolds to smart bioelectronics. Along with the development of new hydrogel systems, 3D printing of such ECHs is one of the most advanced approaches towards rapid fabrication of future biomedical implants and devices with versatile designs and tuneable functionalities. In this review, an overview of the state-of-the-art 3D printed ECHs comprising conductive polymers (polythiophene, polyaniline and polypyrrole) and/or conductive fillers (graphene, MXenes and liquid metals) is provided, with an insight into mechanisms of electrical conductivity and design considerations for tuneable physiochemical properties and biocompatibility. Recent advances in the formulation of 3D printable bioinks and their practical applications are discussed; current challenges and limitations of 3D printing of ECHs are identified; new 3D printing-based hybrid methods for selective deposition and fabrication of controlled nanostructures are highlighted; and finally, future directions are proposed.

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“…Among 3D scaffolds, Li and co-workers [ 246 ] provided an interesting proof of concept of the usage of 3D-printed alginate hydrogels as scaffolds for bone engineering. They used 3D-bioprinting to obtain gelatin-alginate scaffolds with defined porosity, then coated them with RGO.…”

Section: Examples Of Tissue Regenerationmentioning

confidence: 99%

Graphene-Based Scaffolds for Regenerative Medicine

et al. 2021

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Leading-edge regenerative medicine can take advantage of improved knowledge of key roles played, both in stem cell fate determination and in cell growth/differentiation, by mechano-transduction and other physicochemical stimuli from the tissue environment. This prompted advanced nanomaterials research to provide tissue engineers with next-generation scaffolds consisting of smart nanocomposites and/or hydrogels with nanofillers, where balanced combinations of specific matrices and nanomaterials can mediate and finely tune such stimuli and cues. In this review, we focus on graphene-based nanomaterials as, in addition to modulating nanotopography, elastic modulus and viscoelastic features of the scaffold, they can also regulate its conductivity. This feature is crucial to the determination and differentiation of some cell lineages and is of special interest to neural regenerative medicine. Hereafter we depict relevant properties of such nanofillers, illustrate how problems related to their eventual cytotoxicity are solved via enhanced synthesis, purification and derivatization protocols, and finally provide examples of successful applications in regenerative medicine on a number of tissues.

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3D Printing of Cytocompatible Graphene/Alginate Scaffolds for Mimetic Tissue Constructs

Cited by 44 publications

References 47 publications

Applications of 3D printed bone tissue engineering scaffolds in the stem cell field

Applications of 3D printed bone tissue engineering scaffolds in the stem cell field

3D Printable Electrically Conductive Hydrogel Scaffolds for Biomedical Applications: A Review

Graphene-Based Scaffolds for Regenerative Medicine

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