3D Printing Artificial Blood Vessel Constructs Using PCL/Chitosan/Hydrogel Biocomposites

Ulag, Songul; Kalkandelen, Cevriye; Oktar, Faik N.; Uzun, Muhammet; Şahin, Yeşim Müge; Karademir, Betül; Arslan, Sema; Özbolat, İbrahim T.; Mahiroğulları, Mahir; Gündüz, Oğuzhan

doi:10.1002/slct.201803740

Cited by 26 publications

(12 citation statements)

References 12 publications

(15 reference statements)

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“…However, despite the tremendous clinical demand for engineered arterial replacements, the concomitant problems of thrombogenicity, infection resistance, and the ability to repair, remodel, and contract constructed arteries are extremely problematic to achieve [ 268 , 303 , 304 ]. Ulag and colleagues used 3D printing technology to create vessel-like constructions made of Poly (-caprolactone) (PCL), low molecular weight chitosan (CS), and alginate-hyaluronic acid-collagen type I hydrogels to overcome the issues associated with past use of traditional grafts [ 305 ]. This biocompatible, biodegradable, and nonimmunogenic hydrogel provides endothelial cells with a three-dimensional substrate in which to grow.…”

Section: Applications Of 4d Printed Hydrogelsmentioning

confidence: 99%

Smart and Biomimetic 3D and 4D Printed Composite Hydrogels: Opportunities for Different Biomedical Applications

et al. 2021

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In recent years, smart/stimuli-responsive hydrogels have drawn tremendous attention for their varied applications, mainly in the biomedical field. These hydrogels are derived from different natural and synthetic polymers but are also composite with various organic and nano-organic fillers. The basic functions of smart hydrogels rely on their ability to change behavior; functions include mechanical, swelling, shaping, hydrophilicity, and bioactivity in response to external stimuli such as temperature, pH, magnetic field, electromagnetic radiation, and biological molecules. Depending on the final applications, smart hydrogels can be processed in different geometries and modalities to meet the complicated situations in biological media, namely, injectable hydrogels (following the sol-gel transition), colloidal nano and microgels, and three dimensional (3D) printed gel constructs. In recent decades smart hydrogels have opened a new horizon for scientists to fabricate biomimetic customized biomaterials for tissue engineering, cancer therapy, wound dressing, soft robotic actuators, and controlled release of bioactive substances/drugs. Remarkably, 4D bioprinting, a newly emerged technology/concept, aims to rationally design 3D patterned biological matrices from synthesized hydrogel-based inks with the ability to change structure under stimuli. This technology has enlarged the applicability of engineered smart hydrogels and hydrogel composites in biomedical fields. This paper aims to review stimuli-responsive hydrogels according to the kinds of external changes and t recent applications in biomedical and 4D bioprinting.

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Section: Applications Of 4d Printed Hydrogelsmentioning

confidence: 99%

Smart and Biomimetic 3D and 4D Printed Composite Hydrogels: Opportunities for Different Biomedical Applications

et al. 2021

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“…Nevertheless, the usages of these are restricted because of their particular hazardous features. Hence, this procedure (artificial grafts) is a required alternate choice for conquering vascular problems [143]. One of the suggested cure strategies is tissue engineering.…”

Section: Vascular Regenerationmentioning

confidence: 99%

“…Commonly, by addition of hydrogel, the construct elastic modulusand cell attachment and survival will be enhanced. In this regard, the study by Ulag and co-workers [143] can be referred to. In their study, PCL and CS with low Mw and hydrogels (H) were incorporated for preparing the constructs for the enhancement of cells adhesion and spreading owing to the hydrophilic characteristic of hydrogel.…”

Section: Vascular Regenerationmentioning

confidence: 99%

“…Also, the best cell attachment and viability belonged to the PCL/7CS specimen in the presence of the highest hydrogel content (5 wt%). Hence, it seems that the PCL/7CS/5hydrogel is often implemented as a biomaterial for vascular TE [143].…”

Section: Vascular Regenerationmentioning

confidence: 99%

“…Due to the fact that clinical purposes of cell-based approaches are restricted as a result of the expense of keeping cellular constructs on the shelf, possible immune reaction to allogeneic cell lines, and scarce tissue, these acellular scaffolds which may stimulate endogenous influx and uniform distribution of native stem cells from bone marrow could be great candidate for cartilage reproduction [ 109 ]. In this connection, chitosan based constructs have been used for soft tissue regeneration frequently [ 110 , 111 , 112 , 113 , 114 , 115 , 116 , 117 , 118 , 119 , 120 , 121 , 122 , 123 , 124 , 125 , 126 , 127 , 128 , 129 , 130 , 131 , 132 , 133 , 134 , 135 , 136 , 137 , 138 , 139 , 140 , 141 , 142 , 143 , 144 , 145 , 146 , 147 , 148 , 149 , 150 , 151 , 152 , 153 , 154 , …”

Section: Chitosan-based 3d Printed Construct For Hard Tissues Applmentioning

confidence: 99%

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Three-Dimensional Printing Constructs Based on the Chitosan for Tissue Regeneration: State of the Art, Developing Directions and Prospect Trends

et al. 2020

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Chitosan (CS) has gained particular attention in biomedical applications due to its biocompatibility, antibacterial feature, and biodegradability. Hence, many studies have focused on the manufacturing of CS films, scaffolds, particulate, and inks via different production methods. Nowadays, with the possibility of the precise adjustment of porosity size and shape, fiber size, suitable interconnectivity of pores, and creation of patient-specific constructs, 3D printing has overcome the limitations of many traditional manufacturing methods. Therefore, the fabrication of 3D printed CS scaffolds can lead to promising advances in tissue engineering and regenerative medicine. A review of additive manufacturing types, CS-based printed constructs, their usages as biomaterials, advantages, and drawbacks can open doors to optimize CS-based constructions for biomedical applications. The latest technological issues and upcoming capabilities of 3D printing with CS-based biopolymers for different applications are also discussed. This review article will act as a roadmap aiming to investigate chitosan as a new feedstock concerning various 3D printing approaches which may be employed in biomedical fields. In fact, the combination of 3D printing and CS-based biopolymers is extremely appealing particularly with regard to certain clinical purposes. Complications of 3D printing coupled with the challenges associated with materials should be recognized to help make this method feasible for wider clinical requirements. This strategy is currently gaining substantial attention in terms of several industrial biomedical products. In this review, the key 3D printing approaches along with revealing historical background are initially presented, and ultimately, the applications of different 3D printing techniques for fabricating chitosan constructs will be discussed. The recognition of essential complications and technical problems related to numerous 3D printing techniques and CS-based biopolymer choices according to clinical requirements is crucial. A comprehensive investigation will be required to encounter those challenges and to completely understand the possibilities of 3D printing in the foreseeable future.

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Fabrication and In Vitro Characterization of Polycaprolactone/Graphene Oxide/Collagen Nanofibers for Myocardial Repair

Karapehlivan,

Danisik,

Akdag

et al. 2023

Macro Materials & Eng

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This study is focused on fabricating tissue‐engineered electrospun nanofibers that contain polycaprolactone (PCL), graphene oxide (GO), and collagen (COL) to get an alternative treatment for cardiac injuries. GO (1.5 wt%) is used to support the contraction‐elongation of cardiomyocytes by improving electrical stimulation. The COL (1, 3, and 5 wt%) is the main component of the myocardial extracellular matrix have led to their frequent use in cardiac tissue engineering (CTE). The scanning electron microscope (SEM) images show the homogeneous and bead‐free morphologies of the nanofibers. Adding a high amount (3% and 5%) of COL decreases the tensile strength value of 17% PCL/1.5% GO nanofiber. 3‐(4,5‐Dimethylthiazol‐2‐yl)‐2,5‐Diphenyltetrazolium Bromide (MTT) assay demonstrates that the COL addition increases cell viability compared to that in 17% PCL/1.5% GO nanofibers on the third day. The response of the nanofibers to alternating current (AC) signal is studied between the frequencies 40 and 105 Hz. The direct current (DC) conductivity values of the films are determined to be between 1.10−10 and 6.10−10 S m−1 at 25 °C. The AC conductivity values show frequency‐dependent behavior. Among the PCL/GO‐based electrospun nanofibers, 17% PCL/1.5% GO/5% COL nanofiber shows greater DC and AC conductivity than 17% PCL/1.5% GO nanofiber.

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3D Printing Artificial Blood Vessel Constructs Using PCL/Chitosan/Hydrogel Biocomposites

Cited by 26 publications

References 12 publications

Smart and Biomimetic 3D and 4D Printed Composite Hydrogels: Opportunities for Different Biomedical Applications

Smart and Biomimetic 3D and 4D Printed Composite Hydrogels: Opportunities for Different Biomedical Applications

Three-Dimensional Printing Constructs Based on the Chitosan for Tissue Regeneration: State of the Art, Developing Directions and Prospect Trends

Fabrication and In Vitro Characterization of Polycaprolactone/Graphene Oxide/Collagen Nanofibers for Myocardial Repair

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