3D printing and characterization of human nasoseptal chondrocytes laden dual crosslinked oxidized alginate-gelatin hydrogels for cartilage repair approaches

Bioprinting of human nasoseptal chondrocytes‐laden collagen hydrogel for cartilage tissue engineering

“…These limitations may become bottlenecks for further advancements in clinical application. More recently, three‐dimensional (3D) bioprinting approaches employing layer‐by‐layer additive technique allow the creation of custom anatomically shaped cell‐laden engineered cartilages, which may be particularly impactful for patients with large nasal cartilage defect or absent cartilage structures 13,14 . Using a computer‐aided system (CAD), the 3D bioprinting process enables the precise dispensation of the cell‐laden supporting biomaterial (also known as “bioink”) into printed biomimetic construct with a high spatial resolution and homogeneous cell distribution 15,16 …”

Mechanical properties of cell- and microgel bead-laden oxidized alginate-gelatin hydrogels

“…50 We have shown in previous studies by second harmonic generation (SHG) imaging that ADA-GEL does not possess highly ordered fibrillar structures, 84,88 hence can be assumed as a non-fibrillar hydrogel. Its non-fibrous crosslinking structure renders ADA-GEL as an excellent candidate to study the effect of cellular fillers on the mechanical properties of non-fibrous hydrogel matrices, especially as this hydrogel proved its potential for TE 75,76 and biofabrication 82,84,85,89,90 applications.…”

Recent progress in extrusion 3D bioprinting of hydrogel biomaterials for tissue regeneration: a comprehensive review with focus on advanced fabrication techniques

“…For the generation of cartilage constructs, two main strategies of in vitro and in situ bioprinting have been considered in recent years. Employing the in vitro fabrication approach, chondrocytes ,which can be harvested from various zones of the cartilage [197], have been deposited in hydrogels (e.g., gelatin and alginate, alginate, cartilage-dECM, and nanofibrillated cellulose) [198][199][200][201][202] with high cell viability and zone-specific patterns [203,204]. Printing of human chondrocytes in a shear-thinning nanofibrillated cellulose can also be joined with cross-linkable alginate to fabricate anatomically formed cartilage constructs, with high accuracy and permanence [187].…”