3D printed PLA-based scaffolds

Serra, Tiziano; Mateos‐Timoneda, Miguel A.; Planell, Josep A.; Navarro, Melba

doi:10.4161/org.26048

Cited by 162 publications

(55 citation statements)

References 29 publications

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“…This indicates the improvement in osseoconductive properties of nanocomposites. Rapid prototyping structures shows mechanical properties significantly higher than those structures fabricated by other well -known techniques such as solvent -casting and particle leaching, thermal-induced phase separation and gas foaming, among others [4][5][6][7][8]. In this work 3D-printing as an actively developing method for formation of polymer implants was used to produce highly-porous scaffold [1].…”

Section: Introductionmentioning

confidence: 99%

3D-printed scaffolds based on PLA/HA nanocomposites for trabecular bone reconstruction

Niaza

Сенатов

Kaloshkin

et al. 2016

J. Phys.: Conf. Ser.

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Section: Introductionmentioning

confidence: 99%

3D-printed scaffolds based on PLA/HA nanocomposites for trabecular bone reconstruction

Niaza

Сенатов

Kaloshkin

et al. 2016

J. Phys.: Conf. Ser.

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“…The extruded PLA is then printed layer-by-layer, gradually increasing in height over the printing period. We chose to fabricate devices from PLA due to its biocompatibility [24] and compatibility with the MakerGear M2 in contrast with other printer-compatible materials, such as acrylonitrile butadiene styrene (ABS), which is not biocompatible and thus not suitable for working with SC-β cells. A range of parameters including layer thickness, extrusion speeds, and temperatures were explored to produce consistent devices while minimizing the z-axis resolution (Table 1).…”

Section: Resultsmentioning

confidence: 99%

Economic 3D-printing approach for transplantation of human stem cell-derivedβ-like cells

Song

Millman

2016

Biofabrication

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Transplantation of human pluripotent stem cells (hPSC) differentiated into insulin-producing β cells is a regenerative medicine approach being investigated for diabetes cell replacement therapy. This report presents a multifaceted transplantation strategy that combines differentiation into stem cell-derived β (SC-β) cells with 3D printing. By modulating the parameters of a low-cost 3D printer, we created a macroporous device composed of polylactic acid (PLA) that houses SC-β cell clusters within a degradable fibrin gel. Using finite element modeling of cellular oxygen diffusion-consumption and an in vitro culture system that allows for culture of devices at physiological oxygen levels, we identified cluster sizes that avoid severe hypoxia within 3D-printed devices and developed a microwell-based technique for resizing clusters within this range. Upon transplantation into mice, SC-β cell-embedded 3D-printed devices function for 12 weeks, are retrievable, and maintain structural integrity. Here, we demonstrate a novel 3D-printing approach that advances the use of differentiated hPSC for regenerative medicine applications and serves as a platform for future transplantation strategies.

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“…62,63 With a melt temperature around 175 °C, PLA can be easily formed into filaments for use with melt based printing systems where it is generally extruded between 200 and 230 °C. A serious concern surrounding PLA is its long-term biocompatibility because of the release of acidic byproducts during degradation, which could lead to tissue inflammation and cell death.…”

Section: Inks: 3d Printable Biomaterialsmentioning

confidence: 99%

“…168 For instance, Serra et al combined PLA with PEG as a plasticizer and bioactive calcium phosphate glass in chloroform to make a printable polymer-based bioink for DIW bone scaffold construction. 62,66 Primary mesenchymal stem cells (MSC), when cultured on those printed scaffolds, showed increased adhesion to the surface over controls, suggesting the potential for a better migratory and healing response in vivo. The addition of bioactive glass changes the chemical and topographical nature of the scaffold surface in ways that seem to benefit cell progression.…”

Section: Inks: 3d Printable Biomaterialsmentioning

confidence: 99%

Designing Biomaterials for 3D Printing

Guvendiren

Molde

Soares

et al. 2016

ACS Biomater. Sci. Eng.

607

493

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Three-dimensional (3D) printing is becoming an increasingly common technique to fabricate scaffolds and devices for tissue engineering applications. This is due to the potential of 3D printing to provide patient-specific designs, high structural complexity, rapid on-demand fabrication at a low-cost. One of the major bottlenecks that limits the widespread acceptance of 3D printing in biomanufacturing is the lack of diversity in “biomaterial inks”. Printability of a biomaterial is determined by the printing technique. Although a wide range of biomaterial inks including polymers, ceramics, hydrogels and composites have been developed, the field is still struggling with processing of these materials into self-supporting devices with tunable mechanics, degradation, and bioactivity. This review aims to highlight the past and recent advances in biomaterial ink development and design considerations moving forward. A brief overview of 3D printing technologies focusing on ink design parameters is also included.

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3D printed PLA-based scaffolds

Cited by 162 publications

References 29 publications

3D-printed scaffolds based on PLA/HA nanocomposites for trabecular bone reconstruction

3D-printed scaffolds based on PLA/HA nanocomposites for trabecular bone reconstruction

Economic 3D-printing approach for transplantation of human stem cell-derivedβ-like cells

Designing Biomaterials for 3D Printing

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