3D Printed Bioresponsive Devices with Selective Permeability Inspired by Eggshell Membrane for Effective Biochemical Conversion

Abstract

Eggshell membrane has selective permeability that enables gas or liquid molecules to pass through while effectively preventing migration of microbial species. Herein, inspired by the architecture of the eggshell membrane, we employ three-dimensional (3D) printing techniques to realize bioresponsive devices with excellent selective permeability for effective biochemical conversion. The fabricated devices show 3D conductive carbon nanofiber membranes in which precultured microbial cells are controllably deployed. The resulting outcome provides excellent selective permeability between chemical and biological species, which enables acquisition of target responses generated by biological species confined within the device upon input signals. In addition, electrically conductive carbon nanofiber networks provide a platform for real-time monitoring of metabolism of microbial cells in the device. The suggested platform represents an effort to broaden microbial applications by constructing biologically programmed devices for desired responses enabled by designated deployment of engineered cells in a securely confined manner within enclosed membranes using 3D printing methods.

Research on High- and Low-Temperature Characteristics of Bitumen Blended with Waste Eggshell Powder

“…A novel composite nanomaterial, eggshell/Ag, can effectively promote the catalytic degradation of organics and has substantial application prospects in the resolution of problems encountered in microbial treatment of sewage [39]. A 3D-printed biological reactive device was developed based on the selective permeability of eggshell membranes [40]. Eggshells as additive or coating agents for electrode materials prevent electrodes from contacting the electrolyte directly [41].…”

The Next Frontier of 3D Bioprinting: Bioactive Materials Functionalized by Bacteria

“…B) Fabrication of eggshell membrane‐inspired bioresponsive devices using 3D printing Reproduced with permission. [ 69 ] Copyright 2020, American Chemical Society C) Digital design and fabrication of 3D multi‐material structures with programmable biohybrid surfaces. Reproduced with permission.…”

“…Therefore, transgenic microorganisms still have the ability to sense, record, and communicate after encapsulation. Similar to the above design principle, Jeon et al [69] used 3D printing technology to construct a conductive carbon nanofiber membrane similar to an eggshell membrane. In addition to serving as a microbial habitat to prevent microbial detachment and providing good selective permeability between chemistry and biology, the fiber membrane can also be used as a sensor to monitor the metabolism of internal microbial cells (Figure 7B).…”

Recent Progress in 3D Printing of Smart Structures: Classification, Challenges, and Trends

“…Reproduced with permission. [125] Copyright 2020, American Chemical Society; and D) FDM printing of a color-change smart structure that can respond to mechanical deformation and UV light. Reproduced with permission.…”

“…Inspired by the eggshell membrane, a conductive carbon nanofiber membrane with selective permeability fabricated by the DIW method was proposed (Figure 12C). [125] Gregory et al [126] used the DIW method to print poly(ϵ-caprolactone) polymer filaments containing spiropyran. A smart structure capable of mechanical and photochromic discoloration was then fabricated, and its potential application as a sensor capable of visually evaluating peak loads was demonstrated (Figure 12D).…”