3D printed artificial cornea for corneal stromal transplantation

Ulag, Songul; Ilhan, Elif; Şahin, Ali; Yılmaz, Baran; Kalaskar, Deepak M.; Ekren, Nazmi; Kılıç, Osman; Oktar, Faik N.; Gündüz, Oğuzhan

doi:10.1016/j.eurpolymj.2020.109744

Cited by 30 publications

(34 citation statements)

References 39 publications

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“…This can be explained due to the existence of propolis particles, which can prevent the precise orientation of polymer molecules and along with the heterogeneous structure cause a decrease in tensile strength values. If all patches are compared between each other, it can be said that 13% PVA, 13% PVA/0.5% GEL, and 13% PVA/0.5% GEL/3% Ps had acceptable strength values for cornea tissue regeneration (3–5 MPa) [ 47 ]. The Young modulus of the nanofiber patches was calculated using the linear region of the stress/strain curve ( Figure 5 B).…”

Section: Resultsmentioning

confidence: 99%

Propolis-Based Nanofiber Patches to Repair Corneal Microbial Keratitis

et al. 2021

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In this research, polyvinyl-alcohol (PVA)/gelatin (GEL)/propolis (Ps) biocompatible nanofiber patches were fabricated via electrospinning technique. The controlled release of Propolis, surface wettability behaviors, antimicrobial activities against the S. aureus and P. aeruginosa, and biocompatibility properties with the mesenchymal stem cells (MSCs) were investigated in detail. By adding 0.5, 1, and 3 wt.% GEL into the 13 wt.% PVA, the morphological and mechanical results suggested that 13 wt.% PVA/0.5 wt.% GEL patch can be an ideal matrix for 3 and 5 wt.% propolis addition. Morphological results revealed that the diameters of the electrospun nanofiber patches were increased with GEL (from 290 nm to 400 nm) and Ps addition and crosslinking process cause the formation of thicker nanofibers. The tensile strength and elongation at break enhancement were also determined for 13 wt.% PVA/0.5 wt.% GEL/3 wt.% Ps patch. Propolis was released quickly in the first hour and arrived at a plateau. Cell culture and contact angle results confirmed that the 3 wt.% addition of propolis reinforced mesenchymal stem cell proliferation and wettability properties of the patches. The antimicrobial activity demonstrated that propolis loaded patches had antibacterial activity against the S. aureus, but for P. aeruginosa, more studies should be performed.

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Section: Resultsmentioning

confidence: 99%

Propolis-Based Nanofiber Patches to Repair Corneal Microbial Keratitis

et al. 2021

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“…The homogeneous morphology demonstrated high homogeneity between the components. The mean thickness values of the corneal stroma were found 136 μm toward the periphery and 400 μm at the center 68 …”

Section: Characterization Of Bioprinted Hydrogels For Stroma Tissuementioning

confidence: 89%

“…The results indicated that constructs immersed PBS rapidly, and this continued up to a constant swelling rate. The swelling ratio of CS‐added composites was higher than 13%(wt)PVA matrix 68 …”

Section: Characterization Of Bioprinted Hydrogels For Stroma Tissuementioning

confidence: 90%

“…All constructs absorbed the PBS until 3 days, and after this time they started to lose their weights. With CS addition, the degradation rate increased, and 13%PVA/5%CS had higher degradation value 68 …”

Section: Characterization Of Bioprinted Hydrogels For Stroma Tissuementioning

confidence: 97%

“…Extra solution perme O 2 single cell with temperature (23°C) and relative room humidity (60%) system was used to observe the oxygen permeability of the cornea constructs in Ulag et al study 68 . Epoxy was used to glue to corneal constructs onto the metal surface with a hole.…”

Section: Characterization Of Bioprinted Hydrogels For Stroma Tissuementioning

confidence: 99%

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Recent developments and characterization techniques in 3D printing of corneal stroma tissue

Ulag

Uysal

Bedir

et al. 2021

Polymers for Advanced Techs

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Corneal stroma has a significant function in normal visual function. The corneal stroma is vulnerable because of being the thickest part of the cornea, as it can be affected easily by infections or injuries. Any problems on corneal stroma can result in blindness. Donor shortage for corneal transplantation is one of the main issues in corneal transplantation. To address this issue, the corneal tissue engineering focuses on replacing injured tissues and repairing normal functions. Currently, there are no available, engineered corneal tissues for widely accepted routine clinical treatment, but new emerging 3D printing applications are being recognized as a promising option. Recent in vitro researches revealed that the biocompatibility and regeneration possessions of 3D‐printed hydrogels outperformed conventional tissue engineering approaches. The goal of this review is to highlight the current developments in the characterization of 3D cell‐free and bioprinted hydrogels.

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Liquid‐Phase Integrated 3D Printed Biological Lenses for Lamellar Corneal Substitute

Xue

Wang

et al. 2023

Adv Healthcare Materials

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Compared to traditional biological lenses that are used to correct and repair optical systems, such as contact lenses, vision correction surgery, and corneal and lens replacement, three‐dimensional (3D) printed biological lenses can be customized according to user needs. However, the layer‐by‐layer principle of 3D printing leads to a staircase effect, which cannot meet the critical requirements of surface quality during the manufacturing process of biological lens, particularly with soft materials. Here, a liquid‐phase printing strategy and a surface tension‐dependent (STD) post‐processing method are proposed that use the surface tension of the liquid to reconstruct the air‐liquid interface. This eliminates the staircase effect caused by the stacking of units during 3D printing. The coordinates of integrated printing enable high‐accuracy shape control of soft materials. Based on this method, using a typical biological lenses as an example, the surface quality of printed lamellar corneal substitute (LCS) was improved from ±20.0 μm to ±0.2 μm, and the thickness feature size was reduced from ±500 μm to ±150 μm. It can be used to fit the curvature and thickness of the human cornea. The visible light transmittance reaches the level of the natural cornea (∼85%) and is biocompatible. Liquid‐phase 3D printed biological lenses performed better in vivo in rabbit eyes than the molded group. This method can potentially boost research on artificial biological lens printing technology and has good potential for use in future clinical applications.This article is protected by copyright. All rights reserved

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3D printed artificial cornea for corneal stromal transplantation

Cited by 30 publications

References 39 publications

Propolis-Based Nanofiber Patches to Repair Corneal Microbial Keratitis

Propolis-Based Nanofiber Patches to Repair Corneal Microbial Keratitis

Recent developments and characterization techniques in 3D printing of corneal stroma tissue

Liquid‐Phase Integrated 3D Printed Biological Lenses for Lamellar Corneal Substitute

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