3D-printed alginate-hydroxyapatite aerogel scaffolds for bone tissue engineering

Iglesias-Mejuto, Ana; García‐González, Carlos A.

doi:10.1016/j.msec.2021.112525

Cited by 79 publications

(62 citation statements)

References 52 publications

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“…The technological combination of 3D-printing and aerogel processing previously used for the preparation of non-aged alginate-HA aerogels [ 7 ] was herein coupled with extra crosslinking steps (ageing in a CaCl 2 aqueous solution and exposing to GA vapor) to manufacture structures with personalized and enhanced crosslinking degrees. The 3D-printing step allows the customization of the scaffolds while the sc-drying processing results in nanostructured aerogels, thus fulfilling two of the requirements for BTE applications.…”

Section: Resultsmentioning

confidence: 99%

“…However, alginate scaffolds present limitations for bone defect treatment due to their poor bioactivity [ 6 ]. Accordingly, hydroxyapatite (HA) is used as filler in BTE composite scaffolds because of its high biocompatibility, strong reabsorption ability, adequate cell adhesiveness and outstanding bioactivity [ 7 , 8 ].…”

Section: Introductionmentioning

confidence: 99%

“…Nevertheless, the production of aerogels with customized external and internal structures is still a remarkable challenge [ 24 , 25 ]. In this context, the technological combination of 3D-printing technology and supercritical drying results in alginate-HA aerogel-based scaffolds with a high fidelity with respect to the original CAD design and the complex nanostructure required for BTE applications [ 7 ]. Nevertheless, these 3D-printed aerogels should have enhanced properties regarding their degradation rate behavior in biological fluids, their mechanical integrity and their sterility conditions to be compliant with regulations for implantable medical devices.…”

Section: Introductionmentioning

confidence: 99%

“…The recently proposed combination of 3D-printing and supercritical CO 2 technology yielded BTE scaffolds with high biological and structural performance [ 7 ]. A dual crosslinking strategy (CaCl 2 ageing and GA vapor) is herein proposed with the aim of improving the aerogel’s integrity and customization while maintaining its superior performance and fidelity to the original CAD file.…”

Section: Introductionmentioning

confidence: 99%

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3D-Printed, Dual Crosslinked and Sterile Aerogel Scaffolds for Bone Tissue Engineering

Iglesias-Mejuto

García‐González

2022

Polymers

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The fabrication of bioactive three-dimensional (3D) hydrogel scaffolds from biocompatible materials with a complex inner structure (mesoporous and macroporous) and highly interconnected porosity is crucial for bone tissue engineering (BTE). 3D-printing technology combined with aerogel processing allows the fabrication of functional nanostructured scaffolds from polysaccharides for BTE with personalized geometry, porosity and composition. However, these aerogels are usually fragile, with fast biodegradation rates in biological aqueous fluids, and they lack the sterility required for clinical practice. In this work, reinforced alginate-hydroxyapatite (HA) aerogel scaffolds for BTE applications were obtained by a dual strategy that combines extrusion-based 3D-printing and supercritical CO2 gel drying with an extra crosslinking step. Gel ageing in CaCl2 solutions and glutaraldehyde (GA) chemical crosslinking of aerogels were performed as intermediate and post-processing reinforcement strategies to achieve highly crosslinked aerogel scaffolds. Nitrogen adsorption–desorption (BET) and SEM analyses were performed to assess the textural parameters of the resulting alginate-HA aerogel scaffolds. The biological evaluation of the aerogel scaffolds was performed regarding cell viability, hemolytic activity and bioactivity for BTE. The impact of scCO2-based post-sterilization treatment on scaffold properties was also assessed. The obtained aerogels were dual porous, bio- and hemocompatible, as well as endowed with high bioactivity that is dependent on the HA content. This work is a step forward towards the optimization of the physicochemical performance of advanced biomaterials and their sterilization.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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3D-Printed, Dual Crosslinked and Sterile Aerogel Scaffolds for Bone Tissue Engineering

Iglesias-Mejuto

García‐González

2022

Polymers

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“…Iglesias-Mejuto (2021) et al prepared 3D-printed alginate aerogel scaffolds containing HANPs through combination of 3D printing and supercritical CO 2 drying for bone regeneration [ 78 ]. Their results showed that HANPs and CaCl 2 (the major provider of Ca 2+ concentration) determined the scaffold texture.…”

Section: Recent Strategies For Compounding Natural and Synthetic Poly...mentioning

confidence: 99%

Advances of Hydroxyapatite Hybrid Organic Composite Used as Drug or Protein Carriers for Biomedical Applications: A Review

et al. 2022

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Hydroxyapatite (HA), especially in the form of HA nanoparticles (HANPs), has excellent bioactivity, biodegradability, and osteoconductivity and therefore has been widely used as a template or additives for drug delivery in clinical applications, such as dentistry and orthopedic repair. Due to the atomically anisotropic distribution on the preferred growth of HA crystals, especially the nanoscale rod-/whisker-like morphology, HA can generally be a good candidate for carrying a variety of substances. HA is biocompatible and suitable for medical applications, but most drugs carried by HANPs have an initial burst release. In the adsorption mechanism of HA as a carrier, specific surface area, pore size, and porosity are important factors that mainly affect the adsorption and release amounts. At present, many studies have developed HA as a drug carrier with targeted effect, porous structure, and high porosity. This review mainly discusses the influence of HA structures as a carrier on the adsorption and release of active molecules. It then focuses on the benefits and effects of different types of polymer-HA composites to re-examine the proteins/drugs carry and release behavior and related potential clinical applications. This literature survey can be divided into three main parts: 1. interaction and adsorption mechanism of HA and drugs; 2. advantages and application fields of HA/organic composites; 3. loading and drug release behavior of multifunctional HA composites in different environments. This work also presents the latest development and future prospects of HA as a drug carrier.

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Aerogel‐Based Biomaterials for Biomedical Applications: From Fabrication Methods to Disease‐Targeting Applications

et al. 2023

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Aerogel‐based biomaterials are increasingly being considered for biomedical applications due to their unique properties such as high porosity, hierarchical porous network, and large specific pore surface area. Depending on the pore size of the aerogel, biological effects such as cell adhesion, fluid absorption, oxygen permeability, and metabolite exchange can be altered. Based on the diverse potential of aerogels in biomedical applications, this paper provides a comprehensive review of fabrication processes including sol‐gel, aging, drying, and self‐assembly along with the materials that can be used to form aerogels. In addition to the technology utilizing aerogel itself, it also provides insight into the applicability of aerogel based on additive manufacturing technology. To this end, how microfluidic‐based technologies and 3D printing can be combined with aerogel‐based materials for biomedical applications is discussed. Furthermore, previously reported examples of aerogels for regenerative medicine and biomedical applications are thoroughly reviewed. A wide range of applications with aerogels including wound healing, drug delivery, tissue engineering, and diagnostics are demonstrated. Finally, the prospects for aerogel‐based biomedical applications are presented. The understanding of the fabrication, modification, and applicability of aerogels through this study is expected to shed light on the biomedical utilization of aerogels.

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3D-printed alginate-hydroxyapatite aerogel scaffolds for bone tissue engineering

Cited by 79 publications

References 52 publications

3D-Printed, Dual Crosslinked and Sterile Aerogel Scaffolds for Bone Tissue Engineering

3D-Printed, Dual Crosslinked and Sterile Aerogel Scaffolds for Bone Tissue Engineering

Advances of Hydroxyapatite Hybrid Organic Composite Used as Drug or Protein Carriers for Biomedical Applications: A Review

Aerogel‐Based Biomaterials for Biomedical Applications: From Fabrication Methods to Disease‐Targeting Applications

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